MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE

A new MEKC-ESI-MS method for the analysis of amino acids (AAs) in human urine was developed employing ammonium perfluorooctanoate (APFO) as volatile surfactant. The influence of APFO on the MS signal of AAs was evaluated by infusion experiments, which showed that APFO hardly affects analyte response...

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Autores: Moreno-González, David, Sastre Toraño, Javier, Gámiz-Gracia, Laura, García-Campaña, Ana M., de Jong, Gerhardus J., Somsen, Govert W.
Tipo de recurso: artículo
Estado:Versión borrador
Fecha de publicación:2013
País:España
Institución:Universidad de Jaén
Repositorio:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/4249
Acceso en línea:https://hdl.handle.net/10953/4249
Access Level:acceso abierto
Palabra clave:Amino acids
micellar electrokinetic chromatography
mass spectrometry
urine analysis
ammonium perfluorooctanoate
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spelling MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINEMoreno-González, DavidSastre Toraño, JavierGámiz-Gracia, LauraGarcía-Campaña, Ana M.de Jong, Gerhardus J.Somsen, Govert W.Amino acidsmicellar electrokinetic chromatographymass spectrometryurine analysisammonium perfluorooctanoateA new MEKC-ESI-MS method for the analysis of amino acids (AAs) in human urine was developed employing ammonium perfluorooctanoate (APFO) as volatile surfactant. The influence of APFO on the MS signal of AAs was evaluated by infusion experiments, which showed that APFO hardly affects analyte responses and presents significantly less ion suppression than equal concentrations of ammonium acetate. In order to obtain efficient separation of AAs, MEKC parameters such as the pH and APFO concentration of the BGE, were optimized. Optimum AA resolution, including baseline separation of leucine and isoleucine, was obtained using 150 mM APFO (pH 9.0) as BGE, representing a considerable selectivity improvement over CE using 50 mM ammonium acetate (pH 9.0). Optimization of CE-MS parameters, such as sheath liquid composition and flow rate, and ESI and MS settings, led to LODs ranging from 9 to 26 ng/mL for the 20 tested AAs, which is highly favorable for an MEKC-MS method. Good linearity (r2 > 0.99) and repeatability were obtained for all AAs tested with RSD values of 3.0–6.7% for peak area and <1.5% for migration time. The applicability of the MEKC-MS method was demonstrated by the quantitative determination of AAs in urine employing only a 1:1 dilution with BGE as sample pretreatment. All AAs could selectively be detected and quantified obtaining relevant concentration values for normal human urine.Wiley202520252013info:eu-repo/semantics/articleinfo:eu-repo/semantics/draftapplication/mswordhttps://hdl.handle.net/10953/4249reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/42492026-06-24T12:41:07Z
dc.title.none.fl_str_mv MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
title MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
spellingShingle MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
Moreno-González, David
Amino acids
micellar electrokinetic chromatography
mass spectrometry
urine analysis
ammonium perfluorooctanoate
title_short MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
title_full MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
title_fullStr MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
title_full_unstemmed MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
title_sort MICELLAR ELECTROKINETIC CHROMATOGRAPHY–ELECTROSPRAY IONIZATION MASS SPECTROMETRY EMPLOYING A VOLATILE SURFACTANT FOR THE ANALYSIS OF AMINO ACIDS IN HUMAN URINE
dc.creator.none.fl_str_mv Moreno-González, David
Sastre Toraño, Javier
Gámiz-Gracia, Laura
García-Campaña, Ana M.
de Jong, Gerhardus J.
Somsen, Govert W.
author Moreno-González, David
author_facet Moreno-González, David
Sastre Toraño, Javier
Gámiz-Gracia, Laura
García-Campaña, Ana M.
de Jong, Gerhardus J.
Somsen, Govert W.
author_role author
author2 Sastre Toraño, Javier
Gámiz-Gracia, Laura
García-Campaña, Ana M.
de Jong, Gerhardus J.
Somsen, Govert W.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Amino acids
micellar electrokinetic chromatography
mass spectrometry
urine analysis
ammonium perfluorooctanoate
topic Amino acids
micellar electrokinetic chromatography
mass spectrometry
urine analysis
ammonium perfluorooctanoate
description A new MEKC-ESI-MS method for the analysis of amino acids (AAs) in human urine was developed employing ammonium perfluorooctanoate (APFO) as volatile surfactant. The influence of APFO on the MS signal of AAs was evaluated by infusion experiments, which showed that APFO hardly affects analyte responses and presents significantly less ion suppression than equal concentrations of ammonium acetate. In order to obtain efficient separation of AAs, MEKC parameters such as the pH and APFO concentration of the BGE, were optimized. Optimum AA resolution, including baseline separation of leucine and isoleucine, was obtained using 150 mM APFO (pH 9.0) as BGE, representing a considerable selectivity improvement over CE using 50 mM ammonium acetate (pH 9.0). Optimization of CE-MS parameters, such as sheath liquid composition and flow rate, and ESI and MS settings, led to LODs ranging from 9 to 26 ng/mL for the 20 tested AAs, which is highly favorable for an MEKC-MS method. Good linearity (r2 > 0.99) and repeatability were obtained for all AAs tested with RSD values of 3.0–6.7% for peak area and <1.5% for migration time. The applicability of the MEKC-MS method was demonstrated by the quantitative determination of AAs in urine employing only a 1:1 dilution with BGE as sample pretreatment. All AAs could selectively be detected and quantified obtaining relevant concentration values for normal human urine.
publishDate 2013
dc.date.none.fl_str_mv 2013
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/draft
format article
status_str draft
dc.identifier.none.fl_str_mv https://hdl.handle.net/10953/4249
url https://hdl.handle.net/10953/4249
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv CC0 1.0 Universal
http://creativecommons.org/publicdomain/zero/1.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC0 1.0 Universal
http://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/msword
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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