The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures

Producción Científica

Detalhes bibliográficos
Autores: Pérez Martínez, Victor, Lebrero Fernández, Raquel, Muñoz Torre, Raúl, Pérez Fernández, Rebeca
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/68075
Acesso em linha:https://doi.org/10.1016/j.chemosphere.2024.141832
https://uvadoc.uva.es/handle/10324/68075
Access Level:acceso abierto
Palavra-chave:Bioplastics
Greenhouse gas abatement
Methane
Methanotrophic bacteria
pH
PHA
3308 Ingeniería y Tecnología del Medio Ambiente
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spelling The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic culturesPérez Martínez, VictorLebrero Fernández, RaquelMuñoz Torre, RaúlPérez Fernández, RebecaBioplasticsGreenhouse gas abatementMethaneMethanotrophic bacteriapHPHA3308 Ingeniería y Tecnología del Medio AmbienteProducción CientíficaClimate change and plastic pollution are likely the most relevant challenges for the environment in the 21st century. Developing cost-effective technologies for the bioconversion of methane (CH4) into polyhydroxyalkanoates (PHAs) could simultaneously mitigate CH4 emissions and boost the commercialization of biodegradable polymers. Despite the fact that the role of temperature, nitrogen deprivation, CH4:O2 ratio or micronutrients availability on the PHA accumulation capacity of methanotrophs has been carefully explored, there is still a need for optimization of the CH4-to-PHA bioconversion process prior to becoming a feasible platform in future biorefineries. In this study, the influence of different cultivation broth pH values (5.5, 7, 8.5 and 10) on bacterial biomass growth, CH4 bioconversion rate, PHA accumulation capacity and bacterial community structure was investigated in a stirred tank bioreactor under nitrogen deprivation conditions. Higher CH4 elimination rates were obtained at increasing pH, with a maximum value of 50.4 ± 2.7 g CH4·m−3·h−1 observed at pH 8.5. This was likely mediated by an increased ionic strength in the mineral medium, which enhanced the gas-liquid mass transfer. Interestingly, higher PHB accumulations were observed at decreasing pH, with the highest PHB contents recorded at a pH 5.5 (43.7 ± 3.4 %w·w−1). The strong selective pressure of low pH towards the growth of Type II methanotrophic bacteria could explain this finding. The genus Methylocystis increased its abundance from 34 % up to 85 and 90 % at pH 5.5 and 7, respectively. On the contrary, Methylocystis was less abundant in the community enriched at pH 8.5 (14 %). The accumulation of intracellular PHB as energy and carbon storage material allowed the maintenance of high CH4 biodegradation rates during 48 h after complete nitrogen deprivation. The results here obtained demonstrated for the first time a crucial and multifactorial role of pH on the bioconversion performance of CH4 into PHA.Ministerio de Economía y Competitivad/FEDER/Comisión Europea (CTM2015-73228-JIN)Junta de Castilla y León/FEDER (CL-EI-2021-07, UIC315)Elsevier2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.chemosphere.2024.141832https://uvadoc.uva.es/handle/10324/68075reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0045653524007252info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/680752026-06-13T12:44:47Z
dc.title.none.fl_str_mv The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
title The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
spellingShingle The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
Pérez Martínez, Victor
Bioplastics
Greenhouse gas abatement
Methane
Methanotrophic bacteria
pH
PHA
3308 Ingeniería y Tecnología del Medio Ambiente
title_short The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
title_full The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
title_fullStr The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
title_full_unstemmed The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
title_sort The fundamental role of pH in CH4 bioconversion into polyhydroxybutyrate in mixed methanotrophic cultures
dc.creator.none.fl_str_mv Pérez Martínez, Victor
Lebrero Fernández, Raquel
Muñoz Torre, Raúl
Pérez Fernández, Rebeca
author Pérez Martínez, Victor
author_facet Pérez Martínez, Victor
Lebrero Fernández, Raquel
Muñoz Torre, Raúl
Pérez Fernández, Rebeca
author_role author
author2 Lebrero Fernández, Raquel
Muñoz Torre, Raúl
Pérez Fernández, Rebeca
author2_role author
author
author
dc.subject.none.fl_str_mv Bioplastics
Greenhouse gas abatement
Methane
Methanotrophic bacteria
pH
PHA
3308 Ingeniería y Tecnología del Medio Ambiente
topic Bioplastics
Greenhouse gas abatement
Methane
Methanotrophic bacteria
pH
PHA
3308 Ingeniería y Tecnología del Medio Ambiente
description Producción Científica
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.chemosphere.2024.141832
https://uvadoc.uva.es/handle/10324/68075
url https://doi.org/10.1016/j.chemosphere.2024.141832
https://uvadoc.uva.es/handle/10324/68075
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0045653524007252
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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