Using electrochemical methods to study the kinetics of laccase-catalyzed oxidation of phenols
In the present work, the laccase kinetics upon the oxidation process of phenolic compounds was studied using cyclic voltammetry (CV). Several hydroxybenzoic and cinnamic acid compounds were analyzed, and the oxidation kinetics were correlated to their chemical structures. Significant differences wer...
| Autores: | , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/115515 |
| Acesso em linha: | https://hdl.handle.net/2117/115515 https://dx.doi.org/10.1021/acs.iecr.7b04842 |
| Access Level: | acceso abierto |
| Palavra-chave: | Cellulose Cellulose--Oxidation Voltammetry Hydrophobic surfaces Laccase Electrochemical analysis Alkyl gallates Cyclic voltammetry Hydrophobicity Cel·lulosa Cel·lulosa -- Química Voltametria Anàlisi electroquímica Àrees temàtiques de la UPC::Enginyeria paperera |
| Resumo: | In the present work, the laccase kinetics upon the oxidation process of phenolic compounds was studied using cyclic voltammetry (CV). Several hydroxybenzoic and cinnamic acid compounds were analyzed, and the oxidation kinetics were correlated to their chemical structures. Significant differences were observed, and several structural parameters were identified as enhancers of the reaction rate. Folin–Ciocalteau, ultraviolet–visible light (UV-vis) spectrophotometry, and thin-layer chromatography (TLC) methods were also used to assess the laccase-oxidation process. The TLC results correlated well with those from the CV, while the UV-vis results do so with those from the Folin–Ciocalteau analysis. Results suggest that intermediate adducts, nondetectable by CV, Folin–Ciocalteau, UV-vis, and TLC analyses, may be formed during the oxidation process. A new approach for using bare glassy carbon electrodes to detect phenolic compounds and monitor their oxidation using laccase enzymes is presented |
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