Parameters necessary to define an immobilized enzyme preparation
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors,...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versión aceptada para publicación |
| Data de publicação: | 2020 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositório: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/373083 |
| Acesso em linha: | http://hdl.handle.net/10261/373083 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85080029185&doi=10.1016%2fj.procbio.2019.11.026&partnerID=40&md5=eab718a17b25b9490052756f1dd4ca05 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Enzyme immobilization Enzyme inactivation Enzyme stabilization Facts and artifacts Yield determination Batch reactors Enzyme activity Biocatalytic process Diagnostic tests Immobilization method Immobilized enzyme Scientific literature |
| Resumo: | Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors, as well as potential recycle. Whilst much has been reported in the scientific literature about enzyme immobilization methods, in many cases the protocol leads to losses in enzyme activity. In this review we outline the reasons for loss of activity during immobilization and highlight suitable diagnostic tests to elucidate the precise cause and thereby methods to restore activity. The need for standardized reporting of immobilization methods is also emphasized as a means of benchmarking alternative approaches. © 2019 Elsevier Ltd |
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