Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study

Complex polysaccharides such as pectin are receiving increasing attention as emergent prebiotics which could be of interest for human health. Pectin comprises a wide array of highly complex and diverse polysaccharide structures, which are abundant in agricultural by-products. Pectin metabolization b...

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Authors: Calvete-Torre, Inés, Sabater, Carlos, Margolles Barros, Abelardo, Ruíz García, Lorena
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/341244
Online Access:http://hdl.handle.net/10261/341244
Access Level:Open access
Keyword:Prebiotics
Pectin
Microbiota
Apple pomace
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spelling Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic studyCalvete-Torre, InésSabater, CarlosMargolles Barros, AbelardoRuíz García, LorenaPrebioticsPectinMicrobiotaApple pomaceComplex polysaccharides such as pectin are receiving increasing attention as emergent prebiotics which could be of interest for human health. Pectin comprises a wide array of highly complex and diverse polysaccharide structures, which are abundant in agricultural by-products. Pectin metabolization by the gut microbiota requires cooperative metabolism and cross-feeding networks among microbial species. Herein we have deepened into the gut modulatory properties of apple pomaces and pectins derived from this by product, through a shotgun metagenomics approach coupled to advanced functional annotation and prediction of cooperating networks of metagenome-assembled genomes (MAGs). For this purpose, we have used samples from batch in vitro fecal fermentations performed with samples from healthy donors and Crohn's disease patients, in the presence of apple pomace and pectins exhibiting different structural properties. Our results confirm the capacity of apple pomace/pectins to promote key taxa generally underrepresented in Crohn's disease patients but that have been attributed anti-inflammatory properties, Faecalibacterium, Ruminococcaceae members and Akkermansia being very remarkable. Besides, some Ruminococcus and Akkermansia species have been identified as key symbionts, exhibiting complementary metabolic traits to fully metabolise pectins from apple pomace. These results will aid developing microbiome-targeted prebiotic and/or synbiotic strategies for specific population groups.The research performed was funded by the European Union's Horizon2020 Research and Innovation Programme under grant agreement No 818368 (MASTER), and the grants from the Spanish State Research Agency RTI2018-095021-J-I00. CS is a postdoctoral researcher supported by the Juan de la Cierva-Formación Postdoctoral Trainee Program from MICINN, the Spanish Ministry of Science and Innovation (FJC2019-042125-I). Research in our group is also supported by Grant AYUD-2021-50910 from the autonomic Government of Principado de Asturias (FICYT, supported by FEDER).ElsevierEuropean CommissionAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Principado de AsturiasFundación para el Fomento en Asturias de la Investigación Científica Aplicada y la TecnologíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/341244reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/818368info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095021-J-I00info:eu-repo/grantAgreement/AEI//FJC2019-042125-ICalvete-Torre, Inés; Sabater, Carlos ; Margolles Barros, Abelardo ; Ruíz García, Lorena; 2023; Supplementary data Fecal microbiota cooperative metabolism of pectins derived from apple pomace: a functional metagenomic study [Dataset]; Elsevier; https://doi.org/10.1016/j.lwt.2023.115362http://dx.doi.org/10.1016/j.lwt.2023.115362Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3412442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
title Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
spellingShingle Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
Calvete-Torre, Inés
Prebiotics
Pectin
Microbiota
Apple pomace
title_short Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
title_full Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
title_fullStr Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
title_full_unstemmed Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
title_sort Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study
dc.creator.none.fl_str_mv Calvete-Torre, Inés
Sabater, Carlos
Margolles Barros, Abelardo
Ruíz García, Lorena
author Calvete-Torre, Inés
author_facet Calvete-Torre, Inés
Sabater, Carlos
Margolles Barros, Abelardo
Ruíz García, Lorena
author_role author
author2 Sabater, Carlos
Margolles Barros, Abelardo
Ruíz García, Lorena
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Principado de Asturias
Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Prebiotics
Pectin
Microbiota
Apple pomace
topic Prebiotics
Pectin
Microbiota
Apple pomace
description Complex polysaccharides such as pectin are receiving increasing attention as emergent prebiotics which could be of interest for human health. Pectin comprises a wide array of highly complex and diverse polysaccharide structures, which are abundant in agricultural by-products. Pectin metabolization by the gut microbiota requires cooperative metabolism and cross-feeding networks among microbial species. Herein we have deepened into the gut modulatory properties of apple pomaces and pectins derived from this by product, through a shotgun metagenomics approach coupled to advanced functional annotation and prediction of cooperating networks of metagenome-assembled genomes (MAGs). For this purpose, we have used samples from batch in vitro fecal fermentations performed with samples from healthy donors and Crohn's disease patients, in the presence of apple pomace and pectins exhibiting different structural properties. Our results confirm the capacity of apple pomace/pectins to promote key taxa generally underrepresented in Crohn's disease patients but that have been attributed anti-inflammatory properties, Faecalibacterium, Ruminococcaceae members and Akkermansia being very remarkable. Besides, some Ruminococcus and Akkermansia species have been identified as key symbionts, exhibiting complementary metabolic traits to fully metabolise pectins from apple pomace. These results will aid developing microbiome-targeted prebiotic and/or synbiotic strategies for specific population groups.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/341244
url http://hdl.handle.net/10261/341244
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/818368
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095021-J-I00
info:eu-repo/grantAgreement/AEI//FJC2019-042125-I
Calvete-Torre, Inés; Sabater, Carlos ; Margolles Barros, Abelardo ; Ruíz García, Lorena; 2023; Supplementary data Fecal microbiota cooperative metabolism of pectins derived from apple pomace: a functional metagenomic study [Dataset]; Elsevier; https://doi.org/10.1016/j.lwt.2023.115362
http://dx.doi.org/10.1016/j.lwt.2023.115362
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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