Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli

Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously exp...

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Detalhes bibliográficos
Autores: Díaz Del Toro, Silvia, Aguilera, Ángeles, González de Figueras, Carolina, De Francisco Martínez, Patricia, Olsson, Sanna, Puente-Sánchez, Fernando, González-Pastor, José Eduardo
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103047
Acesso em linha:https://hdl.handle.net/20.500.14352/103047
Access Level:acceso abierto
Palavra-chave:579.25
Phytochelatins
Heavy metal resistance
Stress response
Oxidative stress
Bioaccumulation
Microbiología (Biología)
Genética
3108.01 Bacterias
2409 Genética
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oai_identifier_str oai:docta.ucm.es:20.500.14352/103047
network_acronym_str ES
network_name_str España
repository_id_str
spelling Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coliDíaz Del Toro, SilviaAguilera, ÁngelesGonzález de Figueras, CarolinaDe Francisco Martínez, PatriciaOlsson, SannaPuente-Sánchez, FernandoGonzález-Pastor, José Eduardo579.25PhytochelatinsHeavy metal resistanceStress responseOxidative stressBioaccumulationMicrobiología (Biología)Genética3108.01 Bacterias2409 GenéticaPhytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation.MDPIUniversidad Complutense de Madrid20222022-06-2320222022-06-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103047reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1030472026-06-02T12:44:21Z
dc.title.none.fl_str_mv Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
spellingShingle Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
Díaz Del Toro, Silvia
579.25
Phytochelatins
Heavy metal resistance
Stress response
Oxidative stress
Bioaccumulation
Microbiología (Biología)
Genética
3108.01 Bacterias
2409 Genética
title_short Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_full Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_fullStr Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_full_unstemmed Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_sort Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
dc.creator.none.fl_str_mv Díaz Del Toro, Silvia
Aguilera, Ángeles
González de Figueras, Carolina
De Francisco Martínez, Patricia
Olsson, Sanna
Puente-Sánchez, Fernando
González-Pastor, José Eduardo
author Díaz Del Toro, Silvia
author_facet Díaz Del Toro, Silvia
Aguilera, Ángeles
González de Figueras, Carolina
De Francisco Martínez, Patricia
Olsson, Sanna
Puente-Sánchez, Fernando
González-Pastor, José Eduardo
author_role author
author2 Aguilera, Ángeles
González de Figueras, Carolina
De Francisco Martínez, Patricia
Olsson, Sanna
Puente-Sánchez, Fernando
González-Pastor, José Eduardo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.25
Phytochelatins
Heavy metal resistance
Stress response
Oxidative stress
Bioaccumulation
Microbiología (Biología)
Genética
3108.01 Bacterias
2409 Genética
topic 579.25
Phytochelatins
Heavy metal resistance
Stress response
Oxidative stress
Bioaccumulation
Microbiología (Biología)
Genética
3108.01 Bacterias
2409 Genética
description Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-06-23
2022
2022-06-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103047
url https://hdl.handle.net/20.500.14352/103047
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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