Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus

Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, st...

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Autores: Soder-Walz, Jesica M., Torrentó, Clara, Algora, Camelia, Wasmund, Kenneth, Cortés, Pilar, Soler i Gil, Albert, Vicent, Teresa, Rosell, Mònica, Marco-Urrea, E
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/182119
Acceso en línea:https://hdl.handle.net/2445/182119
Access Level:acceso abierto
Palabra clave:Geoquímica
Cloroform
Geochemistry
Chloroform
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spelling Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genusSoder-Walz, Jesica M.Torrentó, ClaraAlgora, CameliaWasmund, KennethCortés, PilarSoler i Gil, AlbertVicent, TeresaRosell, MònicaMarco-Urrea, EGeoquímicaCloroformGeochemistryChloroformTrichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (εC = −2.7 ± 0.1¿ for respiring cells, −3.1 ± 0.1¿ for cell suspensions, and −4.1 ± 0.5¿ for crude protein extracts) and chlorine (εCl = −0.9 ± 0.1¿, −1.1 ± 0.1¿, and −1.2 ± 0.2¿, respectively) isotope fractionation. A characteristic and consistent dual C-Cl isotope fractionation pattern was observed for the three systems (combined ΛC/Cl = 2.8 ± 0.3). This ΛC/Cl differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies.Elsevier B.V.2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion9 p.application/pdfhttps://hdl.handle.net/2445/182119Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.scitotenv.2021.152659Science of the Total Environment, 2021, vol. 813, num. 152659, p. 1-9https://doi.org/10.1016/j.scitotenv.2021.152659cc-by (c) The Authors., 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1821192026-05-29T05:05:01Z
dc.title.none.fl_str_mv Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
title Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
spellingShingle Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
Soder-Walz, Jesica M.
Geoquímica
Cloroform
Geochemistry
Chloroform
title_short Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
title_full Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
title_fullStr Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
title_full_unstemmed Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
title_sort Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
dc.creator.none.fl_str_mv Soder-Walz, Jesica M.
Torrentó, Clara
Algora, Camelia
Wasmund, Kenneth
Cortés, Pilar
Soler i Gil, Albert
Vicent, Teresa
Rosell, Mònica
Marco-Urrea, E
author Soder-Walz, Jesica M.
author_facet Soder-Walz, Jesica M.
Torrentó, Clara
Algora, Camelia
Wasmund, Kenneth
Cortés, Pilar
Soler i Gil, Albert
Vicent, Teresa
Rosell, Mònica
Marco-Urrea, E
author_role author
author2 Torrentó, Clara
Algora, Camelia
Wasmund, Kenneth
Cortés, Pilar
Soler i Gil, Albert
Vicent, Teresa
Rosell, Mònica
Marco-Urrea, E
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Geoquímica
Cloroform
Geochemistry
Chloroform
topic Geoquímica
Cloroform
Geochemistry
Chloroform
description Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (εC = −2.7 ± 0.1¿ for respiring cells, −3.1 ± 0.1¿ for cell suspensions, and −4.1 ± 0.5¿ for crude protein extracts) and chlorine (εCl = −0.9 ± 0.1¿, −1.1 ± 0.1¿, and −1.2 ± 0.2¿, respectively) isotope fractionation. A characteristic and consistent dual C-Cl isotope fractionation pattern was observed for the three systems (combined ΛC/Cl = 2.8 ± 0.3). This ΛC/Cl differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/182119
url https://hdl.handle.net/2445/182119
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.scitotenv.2021.152659
Science of the Total Environment, 2021, vol. 813, num. 152659, p. 1-9
https://doi.org/10.1016/j.scitotenv.2021.152659
dc.rights.none.fl_str_mv cc-by (c) The Authors., 2021
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) The Authors., 2021
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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