Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels

The main aim of the present study was to determine whether differences in the amounts of free cysteine residues in sperm nucleoproteins, which are a direct marker of the integrity of the disulfide bonds between nucleoproteins, existed between good (GFE) and poor boar freezability ejaculates (PFE) du...

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Authors: Yeste Oliveras, Marc, Estrada Paqui, Efrén, Casas Roqueta, Isabel, Bonet, Sergi, Rodríguez-Gil, Joan E
Format: article
Status:Versión aceptada para publicación
Publication Date:2013
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/26283
Online Access:http://hdl.handle.net/10256/26283
Access Level:Open access
Keyword:Estrès oxidatiu
Oxidative stress
Semen -- Crioconservació
Semen -- Cryopreservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Porcs -- Espermatozoides -- Criopreservació
Swine -- Spermatozoa -- Cryopreservation
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oai_identifier_str oai:recercat.cat:10256/26283
network_acronym_str ES
network_name_str España
repository_id_str
spelling Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levelsYeste Oliveras, MarcEstrada Paqui, EfrénCasas Roqueta, IsabelBonet, SergiRodríguez-Gil, Joan EEstrès oxidatiuOxidative stressSemen -- CrioconservacióSemen -- CryopreservationPorcs -- Espermatozoides -- InvestigacióSwine -- Spermatozoa -- ResearchPorcs -- Espermatozoides -- CriopreservacióSwine -- Spermatozoa -- CryopreservationThe main aim of the present study was to determine whether differences in the amounts of free cysteine residues in sperm nucleoproteins, which are a direct marker of the integrity of the disulfide bonds between nucleoproteins, existed between good (GFE) and poor boar freezability ejaculates (PFE) during the different steps of the freeze-thawing process. The analyzed steps were: (1) immediately before starting cryopreservation (17 °C), (2) at the end of the cooling step (5 °C), and (3) 30, and (4) 240 minutes after thawing. In addition, the present study also sought to determine whether GFE and PFE differed in the amounts of peroxides and superoxides generated during freeze-thawing as an overall measure of the boar sperm reactive oxygen species (ROS) accumulation rate. According to our results, PFE present lower resistance than GFE to cryopreservation-induced alterations of disulfide bonds between nucleoproteins, because levels of cysteine free residues were higher in PFE than in GFE at 30 and 240 minutes after thawing. On the other hand, no significant differences were observed between GFE and PFE in ROS levels during freeze-thawing. In conclusion, PFE are less resistant than GFE to cryopreservation not only in terms of sperm motility and membrane integrity, but also in the integrity of nucleoprotein structure. However, this difference between PFE and GFE in the resistance of the nucleoprotein structure to freeze-thawing is not linked with concomitant changes in ROS levelsElsevier2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionpeer-reviewed11 p.application/pdfhttp://hdl.handle.net/10256/26283http://hdl.handle.net/10256/26283© Theriogenology, 2013, vol. 79, núm. 6, p. 929-939Articles publicats (D-B)Yeste Oliveras, Marc Estrada Paqui, Efrén Casas Roqueta, Isabel Bonet, Sergi Rodríguez-Gil, Joan E 2013 Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels Theriogenology 79 6 929 939reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2013.01.008info:eu-repo/semantics/altIdentifier/issn/0093-691Xinfo:eu-repo/semantics/altIdentifier/eissn/1879-3231Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/262832026-05-29T05:05:01Z
dc.title.none.fl_str_mv Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
title Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
spellingShingle Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
Yeste Oliveras, Marc
Estrès oxidatiu
Oxidative stress
Semen -- Crioconservació
Semen -- Cryopreservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Porcs -- Espermatozoides -- Criopreservació
Swine -- Spermatozoa -- Cryopreservation
title_short Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
title_full Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
title_fullStr Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
title_full_unstemmed Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
title_sort Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
dc.creator.none.fl_str_mv Yeste Oliveras, Marc
Estrada Paqui, Efrén
Casas Roqueta, Isabel
Bonet, Sergi
Rodríguez-Gil, Joan E
author Yeste Oliveras, Marc
author_facet Yeste Oliveras, Marc
Estrada Paqui, Efrén
Casas Roqueta, Isabel
Bonet, Sergi
Rodríguez-Gil, Joan E
author_role author
author2 Estrada Paqui, Efrén
Casas Roqueta, Isabel
Bonet, Sergi
Rodríguez-Gil, Joan E
author2_role author
author
author
author
dc.subject.none.fl_str_mv Estrès oxidatiu
Oxidative stress
Semen -- Crioconservació
Semen -- Cryopreservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Porcs -- Espermatozoides -- Criopreservació
Swine -- Spermatozoa -- Cryopreservation
topic Estrès oxidatiu
Oxidative stress
Semen -- Crioconservació
Semen -- Cryopreservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Porcs -- Espermatozoides -- Criopreservació
Swine -- Spermatozoa -- Cryopreservation
description The main aim of the present study was to determine whether differences in the amounts of free cysteine residues in sperm nucleoproteins, which are a direct marker of the integrity of the disulfide bonds between nucleoproteins, existed between good (GFE) and poor boar freezability ejaculates (PFE) during the different steps of the freeze-thawing process. The analyzed steps were: (1) immediately before starting cryopreservation (17 °C), (2) at the end of the cooling step (5 °C), and (3) 30, and (4) 240 minutes after thawing. In addition, the present study also sought to determine whether GFE and PFE differed in the amounts of peroxides and superoxides generated during freeze-thawing as an overall measure of the boar sperm reactive oxygen species (ROS) accumulation rate. According to our results, PFE present lower resistance than GFE to cryopreservation-induced alterations of disulfide bonds between nucleoproteins, because levels of cysteine free residues were higher in PFE than in GFE at 30 and 240 minutes after thawing. On the other hand, no significant differences were observed between GFE and PFE in ROS levels during freeze-thawing. In conclusion, PFE are less resistant than GFE to cryopreservation not only in terms of sperm motility and membrane integrity, but also in the integrity of nucleoprotein structure. However, this difference between PFE and GFE in the resistance of the nucleoprotein structure to freeze-thawing is not linked with concomitant changes in ROS levels
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer-reviewed
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/26283
http://hdl.handle.net/10256/26283
url http://hdl.handle.net/10256/26283
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2013.01.008
info:eu-repo/semantics/altIdentifier/issn/0093-691X
info:eu-repo/semantics/altIdentifier/eissn/1879-3231
dc.rights.none.fl_str_mv Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Theriogenology, 2013, vol. 79, núm. 6, p. 929-939
Articles publicats (D-B)
Yeste Oliveras, Marc Estrada Paqui, Efrén Casas Roqueta, Isabel Bonet, Sergi Rodríguez-Gil, Joan E 2013 Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels Theriogenology 79 6 929 939
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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