The role of dextran production in the metabolic context of Leuconostoc and Weissella Tunisian strains

High molecular weight dextrans improve the rheological properties of fermented products and have immunomodulatory and antiviral activity. We report on 5.84 × 10–2.61 × 10 Da dextrans produced by Leuconostoc lactis AV1n, Weissella cibaria AV2ou and Weissella confusa V30 and FS54 strains. Dextransucra...

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Bibliographic Details
Authors: Besrour-Aouam, Norhane, Fhoula, Imene, Hernández-Alcántara, Annel M., Mohedano Bonillo, Mari Luz, Najjari, Afef, Prieto Orzanco, Alicia, Ruas-Madiedo, Patricia, López García, Paloma, Ouzari, Hadda-Imene
Format: article
Status:Versión aceptada para publicación
Publication Date:2021
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228054
Online Access:http://hdl.handle.net/10261/228054
Access Level:Open access
Keyword:Leuconostoc
Weissella
Dextran
Exopolysaccharides
ucrose metabolism
Bacterial adhesion
Description
Summary:High molecular weight dextrans improve the rheological properties of fermented products and have immunomodulatory and antiviral activity. We report on 5.84 × 10–2.61 × 10 Da dextrans produced by Leuconostoc lactis AV1n, Weissella cibaria AV2ou and Weissella confusa V30 and FS54 strains. Dextransucrases catalyze dextran synthesis by sucrose hydrolysis concomitant with fructose generation. The four bacteria have dextransucrases with molecular weight of about 160 kDa detected by zymograms. Each bacterium showed different interplay of dextran production and metabolic fluxes. All bacteria produced lactate, and AV2ou apart, synthesized mannitol from fructose. FS54 hydrolyzed dextran blue and the concentration of dextran produced by this bacterium decreased during the stationary phase. The AV1n binding to Caco-2 cells and polystyrene plates was higher under conditions for dextran synthesis. Thus, this is the first instance of a Weissella dextranase, associated with a dextransucrase ability, and of a positive influence of dextran on adhesion and aggregation properties of a bacterium.

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