The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach

The influence of stocking density and food deprivation on energy metabolism, stress processes and the pituitary endocrine system of silver catfish (Rhamdia quelen) was investigated after a period of 14 days, in which plasmatic and hepatic parameters and the mRNA expression of prolactin (PRL), growth...

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Autores: Menezes, Charlene C., Ruiz-Jarabo, Ignacio, Martos-Sitcha, Juan Antonio, Toni, Cândida, Salbego, Joseânia, Becker, Alexssandro, Loro, Vania Lucía, Martínez-Rodríguez, Gonzalo, Mancera, Juan Miguel, Baldisserotto, Bernardo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/168560
Acceso en línea:http://hdl.handle.net/10261/168560
Access Level:acceso abierto
Palabra clave:Fish
Hepato-somatic index
Metabolism
Pituitary hormones
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spelling The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approachMenezes, Charlene C.Ruiz-Jarabo, IgnacioMartos-Sitcha, Juan AntonioToni, CândidaSalbego, JoseâniaBecker, AlexssandroLoro, Vania LucíaMartínez-Rodríguez, GonzaloMancera, Juan MiguelBaldisserotto, BernardoFishHepato-somatic indexMetabolismPituitary hormonesThe influence of stocking density and food deprivation on energy metabolism, stress processes and the pituitary endocrine system of silver catfish (Rhamdia quelen) was investigated after a period of 14 days, in which plasmatic and hepatic parameters and the mRNA expression of prolactin (PRL), growth hormone (GH) and somatolactin (SL) were assessed. The fish were subjected to four experimental conditions: (1) fed under high stocking density (32 kg/m3, HSD); (2) fed under mean stocking density (16 kg/m3, MSD); (3) fed under low stocking density (8 kg/m3, LSD); and (4) food-deprived under low stocking density (8 kg/m3, LSD-FD). After 14 days, plasma and liver samples were obtained to analyze the metabolite levels and enzymatic activities related to metabolism, and pituitary glands were obtained to analyze hormone expression (PRL, GH and SL). Liver weight and the hepato-somatic index (HSI) revealed that specimens maintained at HSD and/or MSD had higher hepatic stores, which were observed in the triglyceride and glycogen levels in this tissue, than animals submitted to the LSD and LSD-FD groups. Triglyceride levels in the plasma and liver revealed the consumption of fatty acid reserves in the fasting group. Enzymatic activities, such as glutamate dehydrogenase (GDH), phosphorylase (GPase), pyruvate kinase (PK), aspartate transaminase (AST) and glycerol-3-phosphate dehydrogenase (G3PDH), indicated an increase in gluconeogenic pathways in the HSD group and an increase in glycolitic metabolism in the LSD groups. The expression of PRL was not affected by stocking density and/or food deprivation and GH decreased with increased density and increased in fasting conditions. A negative effect of density and fasting was observed on the expression of SL. Overall, the data suggested that juvenile silver catfish reared at stocking densities of 16 to 32 kg/m3 were better maintained than those maintained at the lowest density.We would like to thank the financial support by CAPES (Ministry of Education of Brazil, Brazil) through the program PDSE-CAPES (Programa Institutional de Bolsas de Doutorado Sanduíche no Exterior — Process: 1585/12-6) to C.M., the CNPq research fellowships to V.L.L. and B.B., the project AGL2010-14876 (Ministry of Science and Education, Spain) to J.M.M. and the program FPU (Formación de Profesorado Universitario — Ref. AP2008-01194) (Ministry of Science and Education, Spain) to JAM-S.Peer reviewedElsevierCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Ministerio de Educación y Ciencia (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/168560reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.aquaculture.2014.09.044Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1685602026-05-22T06:33:51Z
dc.title.none.fl_str_mv The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
title The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
spellingShingle The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
Menezes, Charlene C.
Fish
Hepato-somatic index
Metabolism
Pituitary hormones
title_short The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
title_full The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
title_fullStr The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
title_full_unstemmed The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
title_sort The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach
dc.creator.none.fl_str_mv Menezes, Charlene C.
Ruiz-Jarabo, Ignacio
Martos-Sitcha, Juan Antonio
Toni, Cândida
Salbego, Joseânia
Becker, Alexssandro
Loro, Vania Lucía
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Baldisserotto, Bernardo
author Menezes, Charlene C.
author_facet Menezes, Charlene C.
Ruiz-Jarabo, Ignacio
Martos-Sitcha, Juan Antonio
Toni, Cândida
Salbego, Joseânia
Becker, Alexssandro
Loro, Vania Lucía
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Baldisserotto, Bernardo
author_role author
author2 Ruiz-Jarabo, Ignacio
Martos-Sitcha, Juan Antonio
Toni, Cândida
Salbego, Joseânia
Becker, Alexssandro
Loro, Vania Lucía
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Baldisserotto, Bernardo
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Ministerio de Educación y Ciencia (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fish
Hepato-somatic index
Metabolism
Pituitary hormones
topic Fish
Hepato-somatic index
Metabolism
Pituitary hormones
description The influence of stocking density and food deprivation on energy metabolism, stress processes and the pituitary endocrine system of silver catfish (Rhamdia quelen) was investigated after a period of 14 days, in which plasmatic and hepatic parameters and the mRNA expression of prolactin (PRL), growth hormone (GH) and somatolactin (SL) were assessed. The fish were subjected to four experimental conditions: (1) fed under high stocking density (32 kg/m3, HSD); (2) fed under mean stocking density (16 kg/m3, MSD); (3) fed under low stocking density (8 kg/m3, LSD); and (4) food-deprived under low stocking density (8 kg/m3, LSD-FD). After 14 days, plasma and liver samples were obtained to analyze the metabolite levels and enzymatic activities related to metabolism, and pituitary glands were obtained to analyze hormone expression (PRL, GH and SL). Liver weight and the hepato-somatic index (HSI) revealed that specimens maintained at HSD and/or MSD had higher hepatic stores, which were observed in the triglyceride and glycogen levels in this tissue, than animals submitted to the LSD and LSD-FD groups. Triglyceride levels in the plasma and liver revealed the consumption of fatty acid reserves in the fasting group. Enzymatic activities, such as glutamate dehydrogenase (GDH), phosphorylase (GPase), pyruvate kinase (PK), aspartate transaminase (AST) and glycerol-3-phosphate dehydrogenase (G3PDH), indicated an increase in gluconeogenic pathways in the HSD group and an increase in glycolitic metabolism in the LSD groups. The expression of PRL was not affected by stocking density and/or food deprivation and GH decreased with increased density and increased in fasting conditions. A negative effect of density and fasting was observed on the expression of SL. Overall, the data suggested that juvenile silver catfish reared at stocking densities of 16 to 32 kg/m3 were better maintained than those maintained at the lowest density.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/168560
url http://hdl.handle.net/10261/168560
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.aquaculture.2014.09.044
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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