Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism

13 páginas, 4 figuras, 1 tabla

Detalhes bibliográficos
Autores: Diosdado Martín, Alicia, Simón Marta, Fernando, Morchón, Rodrigo, González Miguel, Javier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/236724
Acesso em linha:http://hdl.handle.net/10261/236724
Access Level:acceso abierto
Palavra-chave:Ascaris suum
Third-stage larvae
Coagulation cascade
Anticoagulants
Factor Xa
Serpin
Larval migration
Ascariosis
Host-parasite relationships
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spelling Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanismDiosdado Martín, AliciaSimón Marta, FernandoMorchón, RodrigoGonzález Miguel, JavierAscaris suumThird-stage larvaeCoagulation cascadeAnticoagulantsFactor XaSerpinLarval migrationAscariosisHost-parasite relationships13 páginas, 4 figuras, 1 tablaIn order to evade the response of their hosts, helminth parasites have evolved precise and highly regulated mechanisms, including migration strategies of the larval stages. In regard to porcine ascariosis caused by Ascaris suum, its infective third-stage larvae (AsL3) undergo a complex migratory route through the bloodstream of their host before establishing in the small intestine to reach maturation. Despite the benefits attributed to this migration, blood clots formation could compromise larvae survival. The aim of this work was to study the interaction between the cuticle and excretory/secretory antigens of AsL3 and the host coagulation cascade. Larvae were obtained after incubating and hatching A. suum eggs, after which the antigenic extracts were produced. Their ability to disrupt the coagulation cascade was studied using anticoagulation and chromogenic assays, and techniques based on electrophoresis. The obtained results showed that both antigenic extracts possessed anticoagulant potential, being able to inhibit the intrinsic, extrinsic and/or common pathways of the blood coagulation cascade as well as the activated factor X. Moreover, three A. suum serpin proteins were identified as candidates to inhibit this host coagulation factor. To the best of our knowledge, this study shows, for the first time, the anticoagulant potential of the infective larvae of A. suum, which could be used by the parasite as a mechanism to facilitate its invasion and survival in the hostThis research was funded by the Centro para el Desarrollo Tecnológico Industrial (CDTI), grant number IDI-2016414. A.D. is supported by a doctoral fellowship from the University of Salamanca, co-funded by Banco Santander. J.G.-M. is supported by the “Juan de la Cierva-Incorporación” program (IJC2018-036660-I) of the Ministerio de Ciencia, Innovación y Universidades (MCIU)Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)González Miguel, Javier [0000-0003-4279-4761]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/236724reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/ani11030804Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2367242026-05-22T06:33:51Z
dc.title.none.fl_str_mv Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
title Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
spellingShingle Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
Diosdado Martín, Alicia
Ascaris suum
Third-stage larvae
Coagulation cascade
Anticoagulants
Factor Xa
Serpin
Larval migration
Ascariosis
Host-parasite relationships
title_short Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
title_full Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
title_fullStr Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
title_full_unstemmed Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
title_sort Host-parasite relationships in porcine ascariosis: Anticoagulant potential of the third larval stage of ascaris suum as a possible survival mechanism
dc.creator.none.fl_str_mv Diosdado Martín, Alicia
Simón Marta, Fernando
Morchón, Rodrigo
González Miguel, Javier
author Diosdado Martín, Alicia
author_facet Diosdado Martín, Alicia
Simón Marta, Fernando
Morchón, Rodrigo
González Miguel, Javier
author_role author
author2 Simón Marta, Fernando
Morchón, Rodrigo
González Miguel, Javier
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
González Miguel, Javier [0000-0003-4279-4761]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ascaris suum
Third-stage larvae
Coagulation cascade
Anticoagulants
Factor Xa
Serpin
Larval migration
Ascariosis
Host-parasite relationships
topic Ascaris suum
Third-stage larvae
Coagulation cascade
Anticoagulants
Factor Xa
Serpin
Larval migration
Ascariosis
Host-parasite relationships
description 13 páginas, 4 figuras, 1 tabla
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/236724
url http://hdl.handle.net/10261/236724
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/ani11030804
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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