A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)

Background: An increasing number of reports are calling our attention to the worldwide spread of leishmaniasis. The urbanization of zoonotic visceral leishmaniasis (VL) has been observed in different South American countries, due to changes in demographic and ecological factors. In May 2006, VL was...

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Autores: Cruz, Israel, Acosta Soto, Lucrecia, Nieto Martinez, Francisco Javier, Cañavate, Carmen, Deschutter, Enrique Jorge, Bornay Llinares, Fernando Jorge
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/34328
Acceso en línea:https://hdl.handle.net/11000/34328
Access Level:acceso abierto
Palabra clave:Leishmaniasis
Domestic dogs
Parasite detection
Leishmania infantum
Leishmania chagasi
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spelling A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)Cruz, IsraelAcosta Soto, LucreciaNieto Martinez, Francisco JavierCañavate, CarmenDeschutter, Enrique JorgeBornay Llinares, Fernando JorgeLeishmaniasisDomestic dogsParasite detectionLeishmania infantumLeishmania chagasiBackground: An increasing number of reports are calling our attention to the worldwide spread of leishmaniasis. The urbanization of zoonotic visceral leishmaniasis (VL) has been observed in different South American countries, due to changes in demographic and ecological factors. In May 2006, VL was detected for the first time in the city of Posadas (Misiones, Argentina). This event encouraged us to conduct a clinical and parasitological pilot survey on domestic dogs from Posadas to identify their potential role as reservoirs for the disease. Methods: One hundred and ten dogs from the city of Posadas were included in the study. They were selected based on convenience and availability. All dogs underwent clinical examination. Symptomatology related to canine leishmaniasis was recorded, and peripheral blood and lymph node aspirates were collected. Anti-Leishmania antibodies were detected using rK39-immunocromatographic tests and IFAT. Parasite detection was based on peripheral blood and lymph node aspirate PCR targeting the SSUrRNA gene. Molecular typing was addressed by DNA sequence analysis of the PCR products obtained by SSUrRNA and ITS-1 PCR. Results: According to clinical examination, 69.1% (76/110) of the dogs presented symptoms compatible with canine leishmaniasis. Serological analyses were positive for 43.6% (48/110) of the dogs and parasite DNA was detected in 47.3% (52/110). A total of 63 dogs (57.3%) were positive by serology and/or PCR. Molecular typing identified Leishmania infantum (syn. Leishmania chagasi) as the causative agent.BioMed CentralDepartamentos de la UMH::Agroquímica y Medio Ambiente202520252010info:eu-repo/semantics/articleapplication/pdf7application/pdfhttps://hdl.handle.net/11000/34328reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1186/1471-2334-10-342info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/343282026-05-27T13:36:21Z
dc.title.none.fl_str_mv A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
title A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
spellingShingle A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
Cruz, Israel
Leishmaniasis
Domestic dogs
Parasite detection
Leishmania infantum
Leishmania chagasi
title_short A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
title_full A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
title_fullStr A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
title_full_unstemmed A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
title_sort A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)
dc.creator.none.fl_str_mv Cruz, Israel
Acosta Soto, Lucrecia
Nieto Martinez, Francisco Javier
Cañavate, Carmen
Deschutter, Enrique Jorge
Bornay Llinares, Fernando Jorge
author Cruz, Israel
author_facet Cruz, Israel
Acosta Soto, Lucrecia
Nieto Martinez, Francisco Javier
Cañavate, Carmen
Deschutter, Enrique Jorge
Bornay Llinares, Fernando Jorge
author_role author
author2 Acosta Soto, Lucrecia
Nieto Martinez, Francisco Javier
Cañavate, Carmen
Deschutter, Enrique Jorge
Bornay Llinares, Fernando Jorge
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Agroquímica y Medio Ambiente
dc.subject.none.fl_str_mv Leishmaniasis
Domestic dogs
Parasite detection
Leishmania infantum
Leishmania chagasi
topic Leishmaniasis
Domestic dogs
Parasite detection
Leishmania infantum
Leishmania chagasi
description Background: An increasing number of reports are calling our attention to the worldwide spread of leishmaniasis. The urbanization of zoonotic visceral leishmaniasis (VL) has been observed in different South American countries, due to changes in demographic and ecological factors. In May 2006, VL was detected for the first time in the city of Posadas (Misiones, Argentina). This event encouraged us to conduct a clinical and parasitological pilot survey on domestic dogs from Posadas to identify their potential role as reservoirs for the disease. Methods: One hundred and ten dogs from the city of Posadas were included in the study. They were selected based on convenience and availability. All dogs underwent clinical examination. Symptomatology related to canine leishmaniasis was recorded, and peripheral blood and lymph node aspirates were collected. Anti-Leishmania antibodies were detected using rK39-immunocromatographic tests and IFAT. Parasite detection was based on peripheral blood and lymph node aspirate PCR targeting the SSUrRNA gene. Molecular typing was addressed by DNA sequence analysis of the PCR products obtained by SSUrRNA and ITS-1 PCR. Results: According to clinical examination, 69.1% (76/110) of the dogs presented symptoms compatible with canine leishmaniasis. Serological analyses were positive for 43.6% (48/110) of the dogs and parasite DNA was detected in 47.3% (52/110). A total of 63 dogs (57.3%) were positive by serology and/or PCR. Molecular typing identified Leishmania infantum (syn. Leishmania chagasi) as the causative agent.
publishDate 2010
dc.date.none.fl_str_mv 2010
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/34328
url https://hdl.handle.net/11000/34328
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1186/1471-2334-10-342
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
7
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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