Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein

15 p.-6 fig.1 graph. abst.

Detalhes bibliográficos
Autores: Machón, Cristina, Ruiz-Masó, José A., Amodio, Juliana, Boer, Roeland, Bordanaba-Ruiseco, Lorena, Bury, Katarzyna, Konieczny, Igor, Solar, Gloria del, Coll, Miquel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/287931
Acesso em linha:http://hdl.handle.net/10261/287931
Access Level:Acceso aberto
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spelling Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function proteinMachón, CristinaRuiz-Masó, José A.Amodio, JulianaBoer, RoelandBordanaba-Ruiseco, LorenaBury, KatarzynaKonieczny, IgorSolar, Gloria delColl, Miquel15 p.-6 fig.1 graph. abst.DNA replication is essential to all living organisms as it ensures the fidelity of genetic material for the next generation of dividing cells. One of the simplest replication initiation mechanisms is the rolling circle replication. In the streptococcal plasmid pMV158, which confers antibiotic resistance to tetracycline, replication initiation is catalysed by RepB protein. The RepB N-terminal domain or origin binding domain binds to the recognition sequence (bind locus) of the double-strand origin of replication and cleaves one DNA strand at a specific site within the nic locus. Using biochemical and crystallographic analyses, here we show how the origin binding domain recognises and binds to the bind locus using structural elements removed from the active site, namely the recognition α helix, and a β-strand that organises upon binding. A new hexameric structure of full-length RepB that highlights the great flexibility of this protein is presented, which could account for its ability to perform different tasks, namely bind to two distinct loci and cleave one strand of DNA at the plasmid origin.Ministry of Science and Innovation of Spain [BFU2017-83720-P, PID2020-120141GB-100 to M.C., 2022AEP028 (RT12018-097114-B-I00), BFU2010-19597 to G.dS.]; RYC fellowship [RYC-2011-09071 to C.M.]; CSIC [2020AEP116 to M.C.]. Funding for open access charge: CSIC.Peer reviewedOxford University PressMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas (España)Machón, Cristina [0000-0001-5363-2958]Ruiz Masó, José A. [0000-0002-7526-8437]Boer, D. Roeland [0000-0001-5949-6627]Bury, Katarzyna [0000-0001-8197-1658]Konieczny, Igor [0000-0002-1588-5601]Solar, Gloria del [0000-0002-7356-9164]Coll, Miquel [0000-0003-4471-8674]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/287931reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-83720-Pinfo:eu-repo/grantAgreement/MICINN//BFU2010-19597info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-120141GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097114-B-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1093/nar/gkac1271https://doi.org/10.1093/nar/gkac1271Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2879312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
title Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
spellingShingle Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
Machón, Cristina
title_short Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
title_full Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
title_fullStr Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
title_full_unstemmed Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
title_sort Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein
dc.creator.none.fl_str_mv Machón, Cristina
Ruiz-Masó, José A.
Amodio, Juliana
Boer, Roeland
Bordanaba-Ruiseco, Lorena
Bury, Katarzyna
Konieczny, Igor
Solar, Gloria del
Coll, Miquel
author Machón, Cristina
author_facet Machón, Cristina
Ruiz-Masó, José A.
Amodio, Juliana
Boer, Roeland
Bordanaba-Ruiseco, Lorena
Bury, Katarzyna
Konieczny, Igor
Solar, Gloria del
Coll, Miquel
author_role author
author2 Ruiz-Masó, José A.
Amodio, Juliana
Boer, Roeland
Bordanaba-Ruiseco, Lorena
Bury, Katarzyna
Konieczny, Igor
Solar, Gloria del
Coll, Miquel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Machón, Cristina [0000-0001-5363-2958]
Ruiz Masó, José A. [0000-0002-7526-8437]
Boer, D. Roeland [0000-0001-5949-6627]
Bury, Katarzyna [0000-0001-8197-1658]
Konieczny, Igor [0000-0002-1588-5601]
Solar, Gloria del [0000-0002-7356-9164]
Coll, Miquel [0000-0003-4471-8674]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 15 p.-6 fig.1 graph. abst.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/287931
url http://hdl.handle.net/10261/287931
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-83720-P
info:eu-repo/grantAgreement/MICINN//BFU2010-19597
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-120141GB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097114-B-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1093/nar/gkac1271
https://doi.org/10.1093/nar/gkac1271
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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