Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis

Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia–legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have b...

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Autores: Fukudome, Mitsutaka, Calvo-Begueria, Laura, Kado, Tomohiro, Osuki, Ken-ichi, Rubio, Maria Carmen, Murakami, Ei-ichi, Nagata, Maki, Kucho, Ken-ichi, Sandal, Niels, Stougaard, Jens, Becana, Manuel, Uchiumi, Toshiki
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:77132
Acesso em linha:http://zaguan.unizar.es/record/77132
Access Level:acceso abierto
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spelling Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosisFukudome, MitsutakaCalvo-Begueria, LauraKado, TomohiroOsuki, Ken-ichiRubio, Maria CarmenMurakami, Ei-ichiNagata, MakiKucho, Ken-ichiSandal, NielsStougaard, JensBecana, ManuelUchiumi, ToshikiLeghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia–legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5′-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo. The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/77132reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/MINECO/AGL2014-53717-Rinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:771322026-05-29T13:59:51Z
dc.title.none.fl_str_mv Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
title Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
spellingShingle Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
Fukudome, Mitsutaka
title_short Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
title_full Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
title_fullStr Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
title_full_unstemmed Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
title_sort Hemoglobin LjGlb1-1 is involved in nodulation and regulated the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
dc.creator.none.fl_str_mv Fukudome, Mitsutaka
Calvo-Begueria, Laura
Kado, Tomohiro
Osuki, Ken-ichi
Rubio, Maria Carmen
Murakami, Ei-ichi
Nagata, Maki
Kucho, Ken-ichi
Sandal, Niels
Stougaard, Jens
Becana, Manuel
Uchiumi, Toshiki
author Fukudome, Mitsutaka
author_facet Fukudome, Mitsutaka
Calvo-Begueria, Laura
Kado, Tomohiro
Osuki, Ken-ichi
Rubio, Maria Carmen
Murakami, Ei-ichi
Nagata, Maki
Kucho, Ken-ichi
Sandal, Niels
Stougaard, Jens
Becana, Manuel
Uchiumi, Toshiki
author_role author
author2 Calvo-Begueria, Laura
Kado, Tomohiro
Osuki, Ken-ichi
Rubio, Maria Carmen
Murakami, Ei-ichi
Nagata, Maki
Kucho, Ken-ichi
Sandal, Niels
Stougaard, Jens
Becana, Manuel
Uchiumi, Toshiki
author2_role author
author
author
author
author
author
author
author
author
author
author
description Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia–legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5′-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo. The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/77132
url http://zaguan.unizar.es/record/77132
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/MINECO/AGL2014-53717-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
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