Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation

Reduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbo...

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Autores: González García, Estefanía, Gutiérrez Ulloa, Carlos Emilio, Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935, Marina Alegre, María Luisa|||0000-0002-5583-1624, García López, María Concepción|||0000-0002-3383-6176
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/44488
Acceso en línea:http://hdl.handle.net/10017/44488
https://dx.doi.org/10.1007/s00216-017-0479-3
Access Level:acceso abierto
Palabra clave:Single-walled carbon nanotubes
Sulfonate carbosilane dendrons
Dendron-coated nanotubes
Protein-dendron interaction
Protein extraction
Química
Chemistry
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spelling Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparationGonzález García, EstefaníaGutiérrez Ulloa, Carlos EmilioMata de la Mata, Francisco Javier de la|||0000-0003-0418-3935Marina Alegre, María Luisa|||0000-0002-5583-1624García López, María Concepción|||0000-0002-3383-6176Single-walled carbon nanotubesSulfonate carbosilane dendronsDendron-coated nanotubesProtein-dendron interactionProtein extractionQuímicaChemistryReduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbon nanotubes (SWCNTs) functionalized with carbosilane dendrons with sulfonate groups for protein sample preparation and shows the successful application of the proposed methodology to extract proteins from a complex matrix. SEM images of nanotubes and mixtures of nanotubes and proteins were taken. Moreover, intrinsic fluorescence intensity of proteins was monitored to observe the most significant interactions at increasing dendron generations under neutral and basic pHs. Different conditions for the disruption of interactions between proteins and nanotubes after protein extraction and different concentrations of the disrupting reagent and the nanotube were also tried. Compatibility of extraction and disrupting conditions with the enzymatic digestion of proteins for obtaining bioactive peptides was also studied. Finally, sulfonate-terminated carbosilane dendron-coated SWCNTs enabled the extraction of proteins from a complex sample without using non-environmentally friendly solvents that were required so far.Comunidad de MadridMinisterio de Economía y Competitividad20172017-07-07journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/44488https://dx.doi.org/10.1007/s00216-017-0479-3reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ-2014-54004-P ESMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2012-36362 Valorización de residuos sólidos vegetales procedentes de la industria agroalimentariaComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available S2013%2FABI-3028 Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentosMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2016-79010-R Desarrollo de métodos sostenibles para el aprovechamiento de subproductos de la industria alimentaria con elevado contenido proteicoopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/444882026-06-18T11:13:07Z
dc.title.none.fl_str_mv Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
title Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
spellingShingle Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
González García, Estefanía
Single-walled carbon nanotubes
Sulfonate carbosilane dendrons
Dendron-coated nanotubes
Protein-dendron interaction
Protein extraction
Química
Chemistry
title_short Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
title_full Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
title_fullStr Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
title_full_unstemmed Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
title_sort Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
dc.creator.none.fl_str_mv González García, Estefanía
Gutiérrez Ulloa, Carlos Emilio
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Marina Alegre, María Luisa|||0000-0002-5583-1624
García López, María Concepción|||0000-0002-3383-6176
author González García, Estefanía
author_facet González García, Estefanía
Gutiérrez Ulloa, Carlos Emilio
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Marina Alegre, María Luisa|||0000-0002-5583-1624
García López, María Concepción|||0000-0002-3383-6176
author_role author
author2 Gutiérrez Ulloa, Carlos Emilio
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Marina Alegre, María Luisa|||0000-0002-5583-1624
García López, María Concepción|||0000-0002-3383-6176
author2_role author
author
author
author
dc.subject.none.fl_str_mv Single-walled carbon nanotubes
Sulfonate carbosilane dendrons
Dendron-coated nanotubes
Protein-dendron interaction
Protein extraction
Química
Chemistry
topic Single-walled carbon nanotubes
Sulfonate carbosilane dendrons
Dendron-coated nanotubes
Protein-dendron interaction
Protein extraction
Química
Chemistry
description Reduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbon nanotubes (SWCNTs) functionalized with carbosilane dendrons with sulfonate groups for protein sample preparation and shows the successful application of the proposed methodology to extract proteins from a complex matrix. SEM images of nanotubes and mixtures of nanotubes and proteins were taken. Moreover, intrinsic fluorescence intensity of proteins was monitored to observe the most significant interactions at increasing dendron generations under neutral and basic pHs. Different conditions for the disruption of interactions between proteins and nanotubes after protein extraction and different concentrations of the disrupting reagent and the nanotube were also tried. Compatibility of extraction and disrupting conditions with the enzymatic digestion of proteins for obtaining bioactive peptides was also studied. Finally, sulfonate-terminated carbosilane dendron-coated SWCNTs enabled the extraction of proteins from a complex sample without using non-environmentally friendly solvents that were required so far.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-07-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/44488
https://dx.doi.org/10.1007/s00216-017-0479-3
url http://hdl.handle.net/10017/44488
https://dx.doi.org/10.1007/s00216-017-0479-3
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ-2014-54004-P ES
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2012-36362 Valorización de residuos sólidos vegetales procedentes de la industria agroalimentaria
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available S2013%2FABI-3028 Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2016-79010-R Desarrollo de métodos sostenibles para el aprovechamiento de subproductos de la industria alimentaria con elevado contenido proteico
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
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