Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile

Clostridiodes difficile can lead to a range of situations from the absence of symptoms (colonization) to severe diarrhea (infection). Disruption of gut microbiota provides an ideal environment for infection to occur. Comparison of gut microbiota of infected and colonized subjects could provide relev...

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Autores: Sanchez-Pellicer, P, Navarro-Lopez, V, Gonzalez-Tamayo, R, Llopis-Ruiz, C, Nunez-Delegido, E, Ruzafa-Costas, B, Navarro-Moratalla, L, Aguera-Santos, J
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p10719
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/10719
Access Level:acceso abierto
Palabra clave:Clostridiodes difficile
Clostridiodes difficile infection
Clostridiodes difficile colonization
gut microbiota
16S ribosomal RNA
dysbiosis
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spelling Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficileSanchez-Pellicer, PNavarro-Lopez, VGonzalez-Tamayo, RLlopis-Ruiz, CNunez-Delegido, ERuzafa-Costas, BNavarro-Moratalla, LAguera-Santos, JClostridiodes difficileClostridiodes difficile infectionClostridiodes difficile colonizationgut microbiota16S ribosomal RNAdysbiosisClostridiodes difficile can lead to a range of situations from the absence of symptoms (colonization) to severe diarrhea (infection). Disruption of gut microbiota provides an ideal environment for infection to occur. Comparison of gut microbiota of infected and colonized subjects could provide relevant information on susceptible groups or protectors to the development of infection, since the presence of certain genera could be related to the inhibition of transition from a state of colonization to infection. Through high-throughput sequencing of 16S rDNA gene, we performed alpha and beta diversity and composition studies on 15 infected patients (Group CDI), 15 colonized subjects (Group P), and 15 healthy controls (Group CTLR). A loss of alpha diversity and richness and a different structure have been evidenced in the CDI and P groups with respect to the CTRL group, but without significant differences between the first two. In CDI and P groups, there was a strong decrease in phylum Firmicutes and an expansion of potential pathogens. Likewise, there was a loss of inhibitory genus of C. difficile germination in infected patients that were partially conserved in colonized subjects. Therefore, infected and colonized subjects presented a gut microbiota that was completely different from that of healthy controls, although similar to each other. It is in composition where we found that colonized subjects, especially in minority genera, presented differences with respect to those infected.MDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/10719MicroorganismsISSN: 20762607reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p107192026-06-11T12:45:17Z
dc.title.none.fl_str_mv Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
title Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
spellingShingle Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
Sanchez-Pellicer, P
Clostridiodes difficile
Clostridiodes difficile infection
Clostridiodes difficile colonization
gut microbiota
16S ribosomal RNA
dysbiosis
title_short Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
title_full Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
title_fullStr Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
title_full_unstemmed Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
title_sort Descriptive Study of Gut Microbiota in Infected and Colonized Subjects by Clostridiodes difficile
dc.creator.none.fl_str_mv Sanchez-Pellicer, P
Navarro-Lopez, V
Gonzalez-Tamayo, R
Llopis-Ruiz, C
Nunez-Delegido, E
Ruzafa-Costas, B
Navarro-Moratalla, L
Aguera-Santos, J
author Sanchez-Pellicer, P
author_facet Sanchez-Pellicer, P
Navarro-Lopez, V
Gonzalez-Tamayo, R
Llopis-Ruiz, C
Nunez-Delegido, E
Ruzafa-Costas, B
Navarro-Moratalla, L
Aguera-Santos, J
author_role author
author2 Navarro-Lopez, V
Gonzalez-Tamayo, R
Llopis-Ruiz, C
Nunez-Delegido, E
Ruzafa-Costas, B
Navarro-Moratalla, L
Aguera-Santos, J
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Clostridiodes difficile
Clostridiodes difficile infection
Clostridiodes difficile colonization
gut microbiota
16S ribosomal RNA
dysbiosis
topic Clostridiodes difficile
Clostridiodes difficile infection
Clostridiodes difficile colonization
gut microbiota
16S ribosomal RNA
dysbiosis
description Clostridiodes difficile can lead to a range of situations from the absence of symptoms (colonization) to severe diarrhea (infection). Disruption of gut microbiota provides an ideal environment for infection to occur. Comparison of gut microbiota of infected and colonized subjects could provide relevant information on susceptible groups or protectors to the development of infection, since the presence of certain genera could be related to the inhibition of transition from a state of colonization to infection. Through high-throughput sequencing of 16S rDNA gene, we performed alpha and beta diversity and composition studies on 15 infected patients (Group CDI), 15 colonized subjects (Group P), and 15 healthy controls (Group CTLR). A loss of alpha diversity and richness and a different structure have been evidenced in the CDI and P groups with respect to the CTRL group, but without significant differences between the first two. In CDI and P groups, there was a strong decrease in phylum Firmicutes and an expansion of potential pathogens. Likewise, there was a loss of inhibitory genus of C. difficile germination in infected patients that were partially conserved in colonized subjects. Therefore, infected and colonized subjects presented a gut microbiota that was completely different from that of healthy controls, although similar to each other. It is in composition where we found that colonized subjects, especially in minority genera, presented differences with respect to those infected.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/10719
url https://fisabio.portalinvestigacion.com/publicaciones/10719
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Microorganisms
ISSN: 20762607
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
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