Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival

Colorectal cancer (CRC) is a biologically heterogeneous disease. To characterize its mutational profile, we conduct targeted sequencing of 205 genes for 2,105 CRC cases with survival data. Our data shows several findings in addition to enhancing the existing knowledge of CRC. We identify PRKCI, SPZ1...

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Autores: Zaidi, Syed H., Harrison, Tabitha A., Phipps, Amanda I., Steinfelder, Robert S., Trinh, Quang M., Qu, Conghui, Banbury, Barbara L., Georgeson, Peter, Grasso, Catherine S., Giannakis, Marios, Adams, Jeremy B., Alwers, Elizabeth, Amitay, Efrat L., Barfield, Richard T., Berndt, Sonja I., Borozan, Ivan, Brenner, Hermann, Brezina, Stefanie, Buchanan, Daniel D., Cao, Yin, Chan, Andrew T., Chang-Claude, Jenny, Connolly, Charles M., Drew, David A., Farris III, Alton Brad, Figueiredo, Jane C., French, Amy J., Fuchs, Charles S., Garraway, Levi A., Gruber, Steve, Guinter, Mark A., Hamilton, Stanley R., Harlid, Sophia, Heisler, Lawrence E., Hidaka, Akihisa, Hopper, John L., Huang, Wen-Yi, Huyghe, Jeroen R., Jenkins, Mark A., Krzyzanowski, Paul M., Lemire, Mathieu, Lin, Yi, Luo, Xuemei, Mardis, Elaine R., McPherson, John D., Miller, Jessica K., Moreno Aguado, Víctor, Mu, Xinmeng Jasmine, Nishihara, Reiko, Papadopoulos, Nikolaos G., Pasternack, Danielle, Quist, Michael J., Rafikova, Adilya, Reid, Emma E. G., Shinbrot, Eve, Shirts, Brian H., Stein, Lincoln D., Teney, Cherie D., Timms, Lee, Um, Caroline Y., Van Guelpen, Bethany, Van Tassel, Megan, Wang, Xiaolong, Wheeler, David A., Yung, Christina K., Hsu, Li, Ogino, Shuji, Gsur, Andrea, Newcomb, Polly A., Gallinger, Steven, Hoffmeister, Michael, Campbell, Peter T., Thibodeau, Stephen N., Sun, Wei, Hudson, Thomas J., Peters, Ulrike
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/173462
Acesso em linha:https://hdl.handle.net/2445/173462
Access Level:Acceso aberto
Palavra-chave:Càncer colorectal
Carcinogènesi
Colorectal cancer
Carcinogenesis
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spelling Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survivalZaidi, Syed H.Harrison, Tabitha A.Phipps, Amanda I.Steinfelder, Robert S.Trinh, Quang M.Qu, ConghuiBanbury, Barbara L.Georgeson, PeterGrasso, Catherine S.Giannakis, MariosAdams, Jeremy B.Alwers, ElizabethAmitay, Efrat L.Barfield, Richard T.Berndt, Sonja I.Borozan, IvanBrenner, HermannBrezina, StefanieBuchanan, Daniel D.Cao, YinChan, Andrew T.Chang-Claude, JennyConnolly, Charles M.Drew, David A.Farris III, Alton BradFigueiredo, Jane C.French, Amy J.Fuchs, Charles S.Garraway, Levi A.Gruber, SteveGuinter, Mark A.Hamilton, Stanley R.Harlid, SophiaHeisler, Lawrence E.Hidaka, AkihisaHopper, John L.Huang, Wen-YiHuyghe, Jeroen R.Jenkins, Mark A.Krzyzanowski, Paul M.Lemire, MathieuLin, YiLuo, XuemeiMardis, Elaine R.McPherson, John D.Miller, Jessica K.Moreno Aguado, VíctorMu, Xinmeng JasmineNishihara, ReikoPapadopoulos, Nikolaos G.Pasternack, DanielleQuist, Michael J.Rafikova, AdilyaReid, Emma E. G.Shinbrot, EveShirts, Brian H.Stein, Lincoln D.Teney, Cherie D.Timms, LeeUm, Caroline Y.Van Guelpen, BethanyVan Tassel, MeganWang, XiaolongWheeler, David A.Yung, Christina K.Hsu, LiOgino, ShujiGsur, AndreaNewcomb, Polly A.Gallinger, StevenHoffmeister, MichaelCampbell, Peter T.Thibodeau, Stephen N.Sun, WeiHudson, Thomas J.Peters, UlrikeCàncer colorectalCarcinogènesiColorectal cancerCarcinogenesisColorectal cancer (CRC) is a biologically heterogeneous disease. To characterize its mutational profile, we conduct targeted sequencing of 205 genes for 2,105 CRC cases with survival data. Our data shows several findings in addition to enhancing the existing knowledge of CRC. We identify PRKCI, SPZ1, MUTYH, MAP2K4, FETUB, and TGFBR2 as additional genes significantly mutated in CRC. We find that among hypermutated tumors, an increased mutation burden is associated with improved CRC-specific survival (HR=0.42, 95% CI: 0.21-0.82). Mutations in TP53 are associated with poorer CRC-specific survival, which is most pronounced in cases carrying TP53 mutations with predicted 0% transcriptional activity (HR=1.53, 95% CI: 1.21-1.94). Furthermore, we observe differences in mutational frequency of several genes and pathways by tumor location, stage, and sex. Overall, this large study provides deep insights into somatic mutations in CRC, and their potential relationships with survival and tumor features. Large scale sequencing study is of paramount importance to unravel the heterogeneity of colorectal cancer. Here, the authors sequenced 205 cancer genes in more than 2000 tumours and identified additional mutated driver genes, determined that mutational burden and specific mutations in TP53 are associated with survival odds.Nature Publishing Group2021202120202021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/173462Articles publicats en revistes (Ciències Clíniques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41467-020-17386-zNature Communications, 2020, vol. 11https://doi.org/10.1038/s41467-020-17386-zcc by (c) Zaidi et al., 2020http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1734622026-05-29T05:05:01Z
dc.title.none.fl_str_mv Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
title Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
spellingShingle Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
Zaidi, Syed H.
Càncer colorectal
Carcinogènesi
Colorectal cancer
Carcinogenesis
title_short Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
title_full Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
title_fullStr Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
title_full_unstemmed Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
title_sort Landscape of somatic single nucleotide variants and indels in colorectal cancer and impact on survival
dc.creator.none.fl_str_mv Zaidi, Syed H.
Harrison, Tabitha A.
Phipps, Amanda I.
Steinfelder, Robert S.
Trinh, Quang M.
Qu, Conghui
Banbury, Barbara L.
Georgeson, Peter
Grasso, Catherine S.
Giannakis, Marios
Adams, Jeremy B.
Alwers, Elizabeth
Amitay, Efrat L.
Barfield, Richard T.
Berndt, Sonja I.
Borozan, Ivan
Brenner, Hermann
Brezina, Stefanie
Buchanan, Daniel D.
Cao, Yin
Chan, Andrew T.
Chang-Claude, Jenny
Connolly, Charles M.
Drew, David A.
Farris III, Alton Brad
Figueiredo, Jane C.
French, Amy J.
Fuchs, Charles S.
Garraway, Levi A.
Gruber, Steve
Guinter, Mark A.
Hamilton, Stanley R.
Harlid, Sophia
Heisler, Lawrence E.
Hidaka, Akihisa
Hopper, John L.
Huang, Wen-Yi
Huyghe, Jeroen R.
Jenkins, Mark A.
Krzyzanowski, Paul M.
Lemire, Mathieu
Lin, Yi
Luo, Xuemei
Mardis, Elaine R.
McPherson, John D.
Miller, Jessica K.
Moreno Aguado, Víctor
Mu, Xinmeng Jasmine
Nishihara, Reiko
Papadopoulos, Nikolaos G.
Pasternack, Danielle
Quist, Michael J.
Rafikova, Adilya
Reid, Emma E. G.
Shinbrot, Eve
Shirts, Brian H.
Stein, Lincoln D.
Teney, Cherie D.
Timms, Lee
Um, Caroline Y.
Van Guelpen, Bethany
Van Tassel, Megan
Wang, Xiaolong
Wheeler, David A.
Yung, Christina K.
Hsu, Li
Ogino, Shuji
Gsur, Andrea
Newcomb, Polly A.
Gallinger, Steven
Hoffmeister, Michael
Campbell, Peter T.
Thibodeau, Stephen N.
Sun, Wei
Hudson, Thomas J.
Peters, Ulrike
author Zaidi, Syed H.
author_facet Zaidi, Syed H.
Harrison, Tabitha A.
Phipps, Amanda I.
Steinfelder, Robert S.
Trinh, Quang M.
Qu, Conghui
Banbury, Barbara L.
Georgeson, Peter
Grasso, Catherine S.
Giannakis, Marios
Adams, Jeremy B.
Alwers, Elizabeth
Amitay, Efrat L.
Barfield, Richard T.
Berndt, Sonja I.
Borozan, Ivan
Brenner, Hermann
Brezina, Stefanie
Buchanan, Daniel D.
Cao, Yin
Chan, Andrew T.
Chang-Claude, Jenny
Connolly, Charles M.
Drew, David A.
Farris III, Alton Brad
Figueiredo, Jane C.
French, Amy J.
Fuchs, Charles S.
Garraway, Levi A.
Gruber, Steve
Guinter, Mark A.
Hamilton, Stanley R.
Harlid, Sophia
Heisler, Lawrence E.
Hidaka, Akihisa
Hopper, John L.
Huang, Wen-Yi
Huyghe, Jeroen R.
Jenkins, Mark A.
Krzyzanowski, Paul M.
Lemire, Mathieu
Lin, Yi
Luo, Xuemei
Mardis, Elaine R.
McPherson, John D.
Miller, Jessica K.
Moreno Aguado, Víctor
Mu, Xinmeng Jasmine
Nishihara, Reiko
Papadopoulos, Nikolaos G.
Pasternack, Danielle
Quist, Michael J.
Rafikova, Adilya
Reid, Emma E. G.
Shinbrot, Eve
Shirts, Brian H.
Stein, Lincoln D.
Teney, Cherie D.
Timms, Lee
Um, Caroline Y.
Van Guelpen, Bethany
Van Tassel, Megan
Wang, Xiaolong
Wheeler, David A.
Yung, Christina K.
Hsu, Li
Ogino, Shuji
Gsur, Andrea
Newcomb, Polly A.
Gallinger, Steven
Hoffmeister, Michael
Campbell, Peter T.
Thibodeau, Stephen N.
Sun, Wei
Hudson, Thomas J.
Peters, Ulrike
author_role author
author2 Harrison, Tabitha A.
Phipps, Amanda I.
Steinfelder, Robert S.
Trinh, Quang M.
Qu, Conghui
Banbury, Barbara L.
Georgeson, Peter
Grasso, Catherine S.
Giannakis, Marios
Adams, Jeremy B.
Alwers, Elizabeth
Amitay, Efrat L.
Barfield, Richard T.
Berndt, Sonja I.
Borozan, Ivan
Brenner, Hermann
Brezina, Stefanie
Buchanan, Daniel D.
Cao, Yin
Chan, Andrew T.
Chang-Claude, Jenny
Connolly, Charles M.
Drew, David A.
Farris III, Alton Brad
Figueiredo, Jane C.
French, Amy J.
Fuchs, Charles S.
Garraway, Levi A.
Gruber, Steve
Guinter, Mark A.
Hamilton, Stanley R.
Harlid, Sophia
Heisler, Lawrence E.
Hidaka, Akihisa
Hopper, John L.
Huang, Wen-Yi
Huyghe, Jeroen R.
Jenkins, Mark A.
Krzyzanowski, Paul M.
Lemire, Mathieu
Lin, Yi
Luo, Xuemei
Mardis, Elaine R.
McPherson, John D.
Miller, Jessica K.
Moreno Aguado, Víctor
Mu, Xinmeng Jasmine
Nishihara, Reiko
Papadopoulos, Nikolaos G.
Pasternack, Danielle
Quist, Michael J.
Rafikova, Adilya
Reid, Emma E. G.
Shinbrot, Eve
Shirts, Brian H.
Stein, Lincoln D.
Teney, Cherie D.
Timms, Lee
Um, Caroline Y.
Van Guelpen, Bethany
Van Tassel, Megan
Wang, Xiaolong
Wheeler, David A.
Yung, Christina K.
Hsu, Li
Ogino, Shuji
Gsur, Andrea
Newcomb, Polly A.
Gallinger, Steven
Hoffmeister, Michael
Campbell, Peter T.
Thibodeau, Stephen N.
Sun, Wei
Hudson, Thomas J.
Peters, Ulrike
author2_role author
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author
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author
author
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dc.subject.none.fl_str_mv Càncer colorectal
Carcinogènesi
Colorectal cancer
Carcinogenesis
topic Càncer colorectal
Carcinogènesi
Colorectal cancer
Carcinogenesis
description Colorectal cancer (CRC) is a biologically heterogeneous disease. To characterize its mutational profile, we conduct targeted sequencing of 205 genes for 2,105 CRC cases with survival data. Our data shows several findings in addition to enhancing the existing knowledge of CRC. We identify PRKCI, SPZ1, MUTYH, MAP2K4, FETUB, and TGFBR2 as additional genes significantly mutated in CRC. We find that among hypermutated tumors, an increased mutation burden is associated with improved CRC-specific survival (HR=0.42, 95% CI: 0.21-0.82). Mutations in TP53 are associated with poorer CRC-specific survival, which is most pronounced in cases carrying TP53 mutations with predicted 0% transcriptional activity (HR=1.53, 95% CI: 1.21-1.94). Furthermore, we observe differences in mutational frequency of several genes and pathways by tumor location, stage, and sex. Overall, this large study provides deep insights into somatic mutations in CRC, and their potential relationships with survival and tumor features. Large scale sequencing study is of paramount importance to unravel the heterogeneity of colorectal cancer. Here, the authors sequenced 205 cancer genes in more than 2000 tumours and identified additional mutated driver genes, determined that mutational burden and specific mutations in TP53 are associated with survival odds.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173462
url https://hdl.handle.net/2445/173462
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41467-020-17386-z
Nature Communications, 2020, vol. 11
https://doi.org/10.1038/s41467-020-17386-z
dc.rights.none.fl_str_mv cc by (c) Zaidi et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Zaidi et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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