The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen
The aim of the present study was to assess the effects of ammonia and water hardness on endocrine, osmoregulatory and metabolic parameters in silver catfish (Rhamdia quelen). The specimens (60-120g) were subjected to six treatments in triplicate, combining three levels of un-ionized ammonia (NH3) (0...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versión aceptada para publicación |
| Data de publicação: | 2014 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositório: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/103820 |
| Acesso em linha: | http://hdl.handle.net/10261/103820 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Ions Growth hormone Metabolites Prolactin Somatolactin Cortisol |
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The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelenBaldisserotto, BernardoMartos-Sitcha, Juan AntonioMenezes, Charlene C.Toni, CândidaPatri, Ricardo L.García, Luciano de O.Salbego, JoseâniaMancera, Juan MiguelMartínez-Rodríguez, GonzaloIonsGrowth hormoneMetabolitesProlactinSomatolactinCortisolThe aim of the present study was to assess the effects of ammonia and water hardness on endocrine, osmoregulatory and metabolic parameters in silver catfish (Rhamdia quelen). The specimens (60-120g) were subjected to six treatments in triplicate, combining three levels of un-ionized ammonia (NH3) (0.020±0.008mg/L [1.17±0.47μM], 0.180±0.020mg/L [10.57±1.17μM] and 0.500±0.007mg/L [29.36±0.41μM]) and two levels of water hardness (normal: 25mgCaCO3/L and high: 120mgCaCO3/L), and sampled after two exposure times (1 and 5 days post-transfer). Plasma cortisol, metabolites, osmolality and ionic values were determined concomitantly with the mRNA expression levels of different adenohypophyseal hormones (growth hormone, GH; prolactin, PRL; and somatolactin, SL). Previously, full-length PRL and SL as well as β-actin cDNAs from R. quelen were cloned. Exposure to high NH3 levels enhanced plasma cortisol levels in fish held under normal water hardness conditions but not in those kept at the high hardness value. The increase in water hardness did not alter plasma metabolites, whereas it modulated the osmolality and ion changes induced by high NH3 levels. However, this hardness increase did not lead to the decreased GH expression that was observed 5 days after exposure to 0.18mg/L NH3 in fish held at the normal water hardness level, whereas PRL expression was enhanced after one day of exposure under the increased hardness conditions. Additionally, SL expression decreased in specimens exposed for 5 days to 0.18mg/L NH3 and maintained at the high water hardness level. The results showed that increasing water hardness attenuated the hormonal parameters evaluated in R. quelen specimens exposed to high NH3 levels, although plasma metabolism do not appear to suffer major changes. © 2014 Elsevier B.V.This study was supported by research funds provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq, process 473718/2011-1) to B. Baldisserotto and by Ministerio de Ciencia y Educación, Spain (process AGL2010-14876) to J. M. Mancera. The authors are also grateful to CNPq for granting a research fellowship to B. Baldisserotto and to Ministerio de Educación, Spain (program “Formación de Profesorado Universitario – FPU, process AP2008-01194) for granting a research fellowship to J. A. Martos-Sitcha.Peer ReviewedElsevierConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Ministerio de Educación y Ciencia (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2014201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/103820reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://doi.org/10.1016/j.aquatox.2014.04.023Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1038202026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| title |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| spellingShingle |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen Baldisserotto, Bernardo Ions Growth hormone Metabolites Prolactin Somatolactin Cortisol |
| title_short |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| title_full |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| title_fullStr |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| title_full_unstemmed |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| title_sort |
The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen |
| dc.creator.none.fl_str_mv |
Baldisserotto, Bernardo Martos-Sitcha, Juan Antonio Menezes, Charlene C. Toni, Cândida Patri, Ricardo L. García, Luciano de O. Salbego, Joseânia Mancera, Juan Miguel Martínez-Rodríguez, Gonzalo |
| author |
Baldisserotto, Bernardo |
| author_facet |
Baldisserotto, Bernardo Martos-Sitcha, Juan Antonio Menezes, Charlene C. Toni, Cândida Patri, Ricardo L. García, Luciano de O. Salbego, Joseânia Mancera, Juan Miguel Martínez-Rodríguez, Gonzalo |
| author_role |
author |
| author2 |
Martos-Sitcha, Juan Antonio Menezes, Charlene C. Toni, Cândida Patri, Ricardo L. García, Luciano de O. Salbego, Joseânia Mancera, Juan Miguel Martínez-Rodríguez, Gonzalo |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) Ministerio de Educación y Ciencia (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Ions Growth hormone Metabolites Prolactin Somatolactin Cortisol |
| topic |
Ions Growth hormone Metabolites Prolactin Somatolactin Cortisol |
| description |
The aim of the present study was to assess the effects of ammonia and water hardness on endocrine, osmoregulatory and metabolic parameters in silver catfish (Rhamdia quelen). The specimens (60-120g) were subjected to six treatments in triplicate, combining three levels of un-ionized ammonia (NH3) (0.020±0.008mg/L [1.17±0.47μM], 0.180±0.020mg/L [10.57±1.17μM] and 0.500±0.007mg/L [29.36±0.41μM]) and two levels of water hardness (normal: 25mgCaCO3/L and high: 120mgCaCO3/L), and sampled after two exposure times (1 and 5 days post-transfer). Plasma cortisol, metabolites, osmolality and ionic values were determined concomitantly with the mRNA expression levels of different adenohypophyseal hormones (growth hormone, GH; prolactin, PRL; and somatolactin, SL). Previously, full-length PRL and SL as well as β-actin cDNAs from R. quelen were cloned. Exposure to high NH3 levels enhanced plasma cortisol levels in fish held under normal water hardness conditions but not in those kept at the high hardness value. The increase in water hardness did not alter plasma metabolites, whereas it modulated the osmolality and ion changes induced by high NH3 levels. However, this hardness increase did not lead to the decreased GH expression that was observed 5 days after exposure to 0.18mg/L NH3 in fish held at the normal water hardness level, whereas PRL expression was enhanced after one day of exposure under the increased hardness conditions. Additionally, SL expression decreased in specimens exposed for 5 days to 0.18mg/L NH3 and maintained at the high water hardness level. The results showed that increasing water hardness attenuated the hormonal parameters evaluated in R. quelen specimens exposed to high NH3 levels, although plasma metabolism do not appear to suffer major changes. © 2014 Elsevier B.V. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2014 2014 2014 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/103820 |
| url |
http://hdl.handle.net/10261/103820 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://doi.org/10.1016/j.aquatox.2014.04.023 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869404840732393472 |
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15,812455 |