Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan

Hydrogels are considered good biomaterials for soft tissue regeneration. In this sense, collagen is the most used raw material to develop hydrogels, due to its high biocompatibility. However, its low mechanical resistance, thermal stability and pH instability have generated the need to look for alte...

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Autores: Sánchez-Cid, Pablo, Jiménez-Rosado, Mercedes, Rubio-Valle, José Fernando, Romero, Alberto, Ostos, Francisco José, Rafii-El-Idrissi Benhnia, Mohamed, Pérez-Puyana, Víctor
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/303864
Acesso em linha:http://hdl.handle.net/10261/303864
https://api.elsevier.com/content/abstract/scopus_id/85122725641
Access Level:acceso abierto
Palavra-chave:Microscopy
Cell viability
Chitosan
Collagen
Hydrogels
Mechanical properties
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spelling Biocompatible and Thermoresistant Hydrogels Based on Collagen and ChitosanSánchez-Cid, PabloJiménez-Rosado, MercedesRubio-Valle, José FernandoRomero, AlbertoOstos, Francisco JoséRafii-El-Idrissi Benhnia, MohamedPérez-Puyana, VíctorMicroscopyCell viabilityChitosanCollagenHydrogelsMechanical propertiesHydrogels are considered good biomaterials for soft tissue regeneration. In this sense, collagen is the most used raw material to develop hydrogels, due to its high biocompatibility. However, its low mechanical resistance, thermal stability and pH instability have generated the need to look for alternatives to its use. In this sense, the combination of collagen with another raw material (i.e., polysaccharides) can improve the final properties of hydrogels. For this reason, the main objective of this work was the development of hydrogels based on collagen and chitosan. The mechanical, thermal and microstructural properties of the hydrogels formed with different ratios of collagen/chitosan (100/0, 75/25, 50/50, 25/75 and 0/100) were evaluated after being processed by two variants of a protocol consisting in two stages: a pH change towards pH 7 and a temperature drop towards 4 °C. The main results showed that depending on the protocol, the physicochemical and microstructural properties of the hybrid hydrogels were similar to the unitary system depending on the stage carried out in first place, obtaining FTIR peaks with similar intensity or a more porous structure when chitosan was first gelled, instead of collagen. As a conclusion, the synergy between collagen and chitosan improved the properties of the hydrogels, showing good thermomechanical properties and cell viability to be used as potential biomaterials for Tissue Engineering.This research was funded by “FEDER/Ministerio de Ciencia e Innovación-Agencia Estatal de Investigación”, though the project RTI2018-097100-B-C21.Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/303864https://api.elsevier.com/content/abstract/scopus_id/85122725641reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097100-B-C21The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/polym14020272https://doi.org/10.3390/polym14020272Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3038642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
title Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
spellingShingle Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
Sánchez-Cid, Pablo
Microscopy
Cell viability
Chitosan
Collagen
Hydrogels
Mechanical properties
title_short Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
title_full Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
title_fullStr Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
title_full_unstemmed Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
title_sort Biocompatible and Thermoresistant Hydrogels Based on Collagen and Chitosan
dc.creator.none.fl_str_mv Sánchez-Cid, Pablo
Jiménez-Rosado, Mercedes
Rubio-Valle, José Fernando
Romero, Alberto
Ostos, Francisco José
Rafii-El-Idrissi Benhnia, Mohamed
Pérez-Puyana, Víctor
author Sánchez-Cid, Pablo
author_facet Sánchez-Cid, Pablo
Jiménez-Rosado, Mercedes
Rubio-Valle, José Fernando
Romero, Alberto
Ostos, Francisco José
Rafii-El-Idrissi Benhnia, Mohamed
Pérez-Puyana, Víctor
author_role author
author2 Jiménez-Rosado, Mercedes
Rubio-Valle, José Fernando
Romero, Alberto
Ostos, Francisco José
Rafii-El-Idrissi Benhnia, Mohamed
Pérez-Puyana, Víctor
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Microscopy
Cell viability
Chitosan
Collagen
Hydrogels
Mechanical properties
topic Microscopy
Cell viability
Chitosan
Collagen
Hydrogels
Mechanical properties
description Hydrogels are considered good biomaterials for soft tissue regeneration. In this sense, collagen is the most used raw material to develop hydrogels, due to its high biocompatibility. However, its low mechanical resistance, thermal stability and pH instability have generated the need to look for alternatives to its use. In this sense, the combination of collagen with another raw material (i.e., polysaccharides) can improve the final properties of hydrogels. For this reason, the main objective of this work was the development of hydrogels based on collagen and chitosan. The mechanical, thermal and microstructural properties of the hydrogels formed with different ratios of collagen/chitosan (100/0, 75/25, 50/50, 25/75 and 0/100) were evaluated after being processed by two variants of a protocol consisting in two stages: a pH change towards pH 7 and a temperature drop towards 4 °C. The main results showed that depending on the protocol, the physicochemical and microstructural properties of the hybrid hydrogels were similar to the unitary system depending on the stage carried out in first place, obtaining FTIR peaks with similar intensity or a more porous structure when chitosan was first gelled, instead of collagen. As a conclusion, the synergy between collagen and chitosan improved the properties of the hydrogels, showing good thermomechanical properties and cell viability to be used as potential biomaterials for Tissue Engineering.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/303864
https://api.elsevier.com/content/abstract/scopus_id/85122725641
url http://hdl.handle.net/10261/303864
https://api.elsevier.com/content/abstract/scopus_id/85122725641
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097100-B-C21
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/polym14020272
https://doi.org/10.3390/polym14020272
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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