Enhancing the expression of starch synthase class IV results in increased levels of both transitory and long-term storage starch

Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of...

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Bibliographic Details
Authors: Gámez-Arjona, Francisco M., Li, Jun, Raynaud, Sandy, Baroja Fernández, Edurne, Muñoz Pérez, Francisco José, Ovecka, Miroslav, Ragel, Paula, Bahaji, Abdellatif, Pozueta Romero, Javier, Mérida, Ángel
Format: article
Status:Published version
Publication Date:2011
Country:España
Institution:Universidad Pública de Navarra
Repository:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/31984
Online Access:https://hdl.handle.net/2454/31984
Access Level:Open access
Keyword:Starch accumulation
Starch synthase
Arabidopsis
Potato
Transgenic plants
Overexpression
Description
Summary:Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP‐glucose or ATP available for starch biosynthesis. In this work, we show that the overexpression of starch synthase class IV (SSIV) increases the levels of starch accumulated in the leaves of Arabidopsis by 30%–40%. In addition, SSIV‐overexpressing lines display a higher rate of growth. The increase in starch content as a consequence of enhanced SSIV expression is also observed in long‐term storage starch organs such as potato tubers. Overexpression of SSIV in potato leads to increased tuber starch content on a dry weight basis and to increased yield of starch production in terms of tons of starch/hectare. These results identify SSIV as one of the regulatory steps involved in the control of the amount of starch accumulated in plastids.

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