Community recommendations on cryoEM data archiving and validation

In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and arch...

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Detalhes bibliográficos
Autores: Kleywegt, Gerard J., Adams, Paul D., Butcher, Sarah J., Lawson, Catherine L., Rohou, Alexis, Rosenthal, Peter B., Subramaniam, Sriram, Topf, Maya, Abbott, Sanja, Baldwin, Philip R., Berrisford, John M., Bricogne, Gérard, Choudhary, Preeti, Croll, Tristan I., Danev, Radostin, Ganesan, Sai J., Grant, Timothy, Gutmanas, Aleksandras, Henderson, Richard, Heymann, J. Bernard, Huiskonen, Juha T., Istrate, Andrei, Kato, Takayuki, Lander, Gabriel C., Lok, Shee-Mei, Ludtke, Steven J., Murshudov, Garib N., Pye, Ryan, Pintilie, Grigore D., Richardson, Jane S., Sachse, Carsten, Salih, Osman, Scheres, Sjors H. W., Schroeder, Gunnar F., Sorzano, Carlos Óscar S., Stagg, Scott M., Wang, Zhe, Warshamanage, Rangana, Westbrook, John D., Winn, Martyn D., Young, Jasmine Y., Burley, Stephen K., Hoch, Jeffrey C., Kurisu, Genji, Morris, Kyle, Patwardhan, Ardan, Velankar, Sameer
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/358564
Acesso em linha:http://hdl.handle.net/10261/358564
Access Level:Acceso aberto
Palavra-chave:Cryogenic-specimen electron microscopy
Data archiving
Validation
Quality control
Electron Microscopy Data Bank
Protein Data Bank
Single-particle cryoEM
Databases
Bioinformatics
Structure determination
Descrição
Resumo:In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and archiving of such structures. This report describes the workshop’s motivation and history, the topics discussed, and the resulting consensus recommendations. Some challenges for future methods-development efforts in this area are also highlighted, as is the implementation to date of some of the recommendations.

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