Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate

Aquaporins (AQPs) are transmembrane channels with permeability to water and small solutes that can be classified according to their structure and permeability into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs. In boar spermatozoa, AQPs are related to osmoregulation and play a critical role...

Descripción completa

Detalles Bibliográficos
Autores: Delgado Bermúdez, Ariadna, Llavanera, Marc, Recuero, Sandra, Mateo Otero, Yentel, Bonet, Sergi, Barranco, Isabel, Fernández Fuertes, Beatriz, Yeste Oliveras, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/17329
Acceso en línea:http://hdl.handle.net/10256/17329
Access Level:acceso abierto
Palabra clave:Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Aquaporines
Aquaporins
id ES_1f6d55619acf5fb05af7cf1f829c17c0
oai_identifier_str oai:recercat.cat:10256/17329
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the EjaculateDelgado Bermúdez, AriadnaLlavanera, MarcRecuero, SandraMateo Otero, YentelBonet, SergiBarranco, IsabelFernández Fuertes, BeatrizYeste Oliveras, MarcSenglar -- Espermatozoides -- CriopreservacióWild boar -- Spermatozoa -- CryopreservationAquaporinesAquaporinsAquaporins (AQPs) are transmembrane channels with permeability to water and small solutes that can be classified according to their structure and permeability into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs. In boar spermatozoa, AQPs are related to osmoregulation and play a critical role in maturation and motility activation. In addition, their levels differ between ejaculates with good and poor cryotolerance (GFE and PFE, respectively). The aim of this work was to elucidate whether the involvement of AQPs in the sperm response to cryopreservation relies on the intrinsic freezability of the ejaculate. With this purpose, two different molecules: phloretin (PHL) and 1,3-propanediol (PDO), were used to inhibit sperm AQPs in GFE and PFE. Boar sperm samples were treated with three different concentrations of each inhibitor prior to cryopreservation, and sperm quality and functionality parameters were evaluated in fresh samples and after 30 and 240 min of thawing. Ejaculates were classified as GFE or PFE, according to their post-thaw sperm viability and motility. While the presence of PHL caused a decrease in sperm quality and function compared to the control, samples treated with PDO exhibited better quality and function parameters than the control. In addition, the effects of both inhibitors were more apparent in GFE than in PFE. In conclusion, AQP inhibition has more notable consequences in GFE than in PFE, which can be related to the difference in relative levels of AQPs between these two groups of samplesThe authors acknowledge the support from the European Commission (H2020-MSCA-IF-79212), the Ministry of Science, Innovation and Universities, Spain (grant nos. RYC-2014-15581, AGL2017-88329-R, FJCI-2017-31689, and PRE2018-083488), and the Regional Government of Catalonia, Spain (2017-SGR-1229)MDPI (Multidisciplinary Digital Publishing Institute)Agencia Estatal de InvestigaciónMinisterio de Economía y Competitividad (Espanya)2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/17329http://hdl.handle.net/10256/17329International Journal of Molecular Sciences, 2019, vol. 20, núm. 24, p. 6255Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20246255info:eu-repo/semantics/altIdentifier/issn/1661-6596info:eu-repo/semantics/altIdentifier/eissn/1422-0067info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-Rinfo:eu-repo/grantAgreement/MINECO//RYC-2014-15581Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/173292026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
title Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
spellingShingle Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
Delgado Bermúdez, Ariadna
Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Aquaporines
Aquaporins
title_short Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
title_full Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
title_fullStr Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
title_full_unstemmed Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
title_sort Effect of AQP Inhibition on Boar Sperm Cryotolerance Depends on the Intrinsic Freezability of the Ejaculate
dc.creator.none.fl_str_mv Delgado Bermúdez, Ariadna
Llavanera, Marc
Recuero, Sandra
Mateo Otero, Yentel
Bonet, Sergi
Barranco, Isabel
Fernández Fuertes, Beatriz
Yeste Oliveras, Marc
author Delgado Bermúdez, Ariadna
author_facet Delgado Bermúdez, Ariadna
Llavanera, Marc
Recuero, Sandra
Mateo Otero, Yentel
Bonet, Sergi
Barranco, Isabel
Fernández Fuertes, Beatriz
Yeste Oliveras, Marc
author_role author
author2 Llavanera, Marc
Recuero, Sandra
Mateo Otero, Yentel
Bonet, Sergi
Barranco, Isabel
Fernández Fuertes, Beatriz
Yeste Oliveras, Marc
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Aquaporines
Aquaporins
topic Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Aquaporines
Aquaporins
description Aquaporins (AQPs) are transmembrane channels with permeability to water and small solutes that can be classified according to their structure and permeability into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs. In boar spermatozoa, AQPs are related to osmoregulation and play a critical role in maturation and motility activation. In addition, their levels differ between ejaculates with good and poor cryotolerance (GFE and PFE, respectively). The aim of this work was to elucidate whether the involvement of AQPs in the sperm response to cryopreservation relies on the intrinsic freezability of the ejaculate. With this purpose, two different molecules: phloretin (PHL) and 1,3-propanediol (PDO), were used to inhibit sperm AQPs in GFE and PFE. Boar sperm samples were treated with three different concentrations of each inhibitor prior to cryopreservation, and sperm quality and functionality parameters were evaluated in fresh samples and after 30 and 240 min of thawing. Ejaculates were classified as GFE or PFE, according to their post-thaw sperm viability and motility. While the presence of PHL caused a decrease in sperm quality and function compared to the control, samples treated with PDO exhibited better quality and function parameters than the control. In addition, the effects of both inhibitors were more apparent in GFE than in PFE. In conclusion, AQP inhibition has more notable consequences in GFE than in PFE, which can be related to the difference in relative levels of AQPs between these two groups of samples
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/17329
http://hdl.handle.net/10256/17329
url http://hdl.handle.net/10256/17329
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20246255
info:eu-repo/semantics/altIdentifier/issn/1661-6596
info:eu-repo/semantics/altIdentifier/eissn/1422-0067
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv International Journal of Molecular Sciences, 2019, vol. 20, núm. 24, p. 6255
Articles publicats (D-B)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404400063086593
score 15,223283

Similares en LA Referencia