Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro

9 pages, 8 figures.-- PMID: 8797853 [PubMed].-- Available online Jul 23, 2004.

Detalhes bibliográficos
Autores: Ruíz Lara, Simón A., Cornudella, Lluís, Rodríguez Campos, Antonio
Formato: artículo
Fecha de publicación:1996
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/27579
Acesso em linha:http://hdl.handle.net/10261/27579
Access Level:acceso abierto
Palavra-chave:Chromatin assembly
Nucleoplasmin
Oocyte S150 extract
Sperm decondensation
Sperm protein–DNA interactions
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spelling Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitroRuíz Lara, Simón A.Cornudella, LluísRodríguez Campos, AntonioChromatin assemblyNucleoplasminOocyte S150 extractSperm decondensationSperm protein–DNA interactions9 pages, 8 figures.-- PMID: 8797853 [PubMed].-- Available online Jul 23, 2004.Nucleoplasmin, an acidic thermostable protein abundant in the nucleus of Xenopus laevis oocytes, has been found to dissociate complexes of pUC19 DNA and protein φ1, an intermediate protamine present in ripe sperm from the mollusc Mytilus edulis. Cruder preparations of nucleoplasmin, such as the amphibian oocyte S150 extract and its thermostable fraction, also dissociate the heterologous DNA-φ1 complexes and, in addition, promote the assembly of plasmid DNA into a minichromosome displaying regular nucleosomal periodicity, as revealed by micrococcal nuclease digestion. In contrast, purified nucleoplasmin complemented with rat hepatocyte core histone octamers in the presence of DNA topoisomerase I, although capable of inducing nucleoprotein formation onto the complexed DNA, fails to position nucleosomes at the native spacings seen in chromatin in vivo. These data favour the existence of a general mechanism to bring about, in a concerted manner, removal of sperm specific nuclear proteins and reconstitution of somatic chromatin following fertilization.This work was supported in part by grant PB94-0042 from the Spanish Direccidn General de Investigación Científica y Técnica and by the NATO Scientific Affairs Division grant CRG93-1146 (to Luis Cornudella).Peer reviewedBlackwell Publishing201020101996info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65012260070 bytesapplication/pdfhttp://hdl.handle.net/10261/27579reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1432-1033.1996.0186h.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/275792026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
title Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
spellingShingle Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
Ruíz Lara, Simón A.
Chromatin assembly
Nucleoplasmin
Oocyte S150 extract
Sperm decondensation
Sperm protein–DNA interactions
title_short Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
title_full Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
title_fullStr Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
title_full_unstemmed Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
title_sort Dissociation of protamine-DNA complexes by Xenopus nucleoplasmin and minichromosome assembly in vitro
dc.creator.none.fl_str_mv Ruíz Lara, Simón A.
Cornudella, Lluís
Rodríguez Campos, Antonio
author Ruíz Lara, Simón A.
author_facet Ruíz Lara, Simón A.
Cornudella, Lluís
Rodríguez Campos, Antonio
author_role author
author2 Cornudella, Lluís
Rodríguez Campos, Antonio
author2_role author
author
dc.subject.none.fl_str_mv Chromatin assembly
Nucleoplasmin
Oocyte S150 extract
Sperm decondensation
Sperm protein–DNA interactions
topic Chromatin assembly
Nucleoplasmin
Oocyte S150 extract
Sperm decondensation
Sperm protein–DNA interactions
description 9 pages, 8 figures.-- PMID: 8797853 [PubMed].-- Available online Jul 23, 2004.
publishDate 1996
dc.date.none.fl_str_mv 1996
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/27579
url http://hdl.handle.net/10261/27579
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1432-1033.1996.0186h.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2260070 bytes
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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