Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis

The development of low-cost, rapid point-of-care tests (POCT) for the detection of the parasite Trichomonas vaginalis is listed in the recent WHO global research priorities for sexually transmitted infections (STIs). Aiming to contribute to this call and aid in ending STIs as a public health threat,...

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Detalhes bibliográficos
Autores: Ibáñez Escribano, Alexandra, Justo, Christine Aubrey C., Skouridou, Vasso, Cools, Piet, Mulinganya, Guy, O´Sullivan, Ciara K.
Tipo de documento: artigo
Data de publicação:2025
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/120130
Acesso em linha:https://hdl.handle.net/20.500.14352/120130
Access Level:Acceso aberto
Palavra-chave:616.9
616.99
579.66
615.28
Ciencias Biomédicas
Enfermedades infecciosas
Parasitología (Farmacia)
Microbiología (Farmacia)
Microbiología médica
32 Ciencias Médicas
3207.12 Parasitología
3201.03 Microbiología Clínica
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spelling Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalisIbáñez Escribano, AlexandraJusto, Christine Aubrey C.Skouridou, VassoCools, PietMulinganya, GuyO´Sullivan, Ciara K.616.9616.99579.66615.28Ciencias BiomédicasEnfermedades infecciosasParasitología (Farmacia)Microbiología (Farmacia)Microbiología médica32 Ciencias Médicas3207.12 Parasitología3201.03 Microbiología ClínicaThe development of low-cost, rapid point-of-care tests (POCT) for the detection of the parasite Trichomonas vaginalis is listed in the recent WHO global research priorities for sexually transmitted infections (STIs). Aiming to contribute to this call and aid in ending STIs as a public health threat, we report the development of a DNA aptamer-based POCT for T. vaginalis. Herein, we report an aptamer-based lateral flow assay (ALFA) based on dual aptamers for the detection of T. vaginalis. This is the first report of an ALFA that does not rely on biotin/streptavidin for either capture or reporter molecule. Aptamer capture and recognition relies on the use of an UV-crosslinked aminated capture aptamer on the nitrocellulose membrane and gold nanoparticles (AuNPs) functionalized with a thioctic acid-modified reporter aptamer. The developed ALFA has an estimated laboratory production cost of less than 1 € per test, with the running buffer and the ALFA strip stable for at least 1 year at room temperature (22 °C). The assay requires three simple operator steps from sample procurement to result with a 15-minute runtime. The developed ALFA can detect down to 1.6 × 105 T. vaginalis per mL and shows no cross-reactivity to common vaginal microorganisms and no matrix effect from clinical cervicovaginal lavage (CVL) samples is observed. Preliminary clinical evaluation using CVLs demonstrated that the assay has perfect concordance with wetmount assay on matching vaginal swabs. The developed rapid test offers a simple, stable, and low-cost diagnostic test for T. vaginalis.Royal Society of ChemistryUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/120130reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1201302026-06-02T12:44:21Z
dc.title.none.fl_str_mv Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
title Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
spellingShingle Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
Ibáñez Escribano, Alexandra
616.9
616.99
579.66
615.28
Ciencias Biomédicas
Enfermedades infecciosas
Parasitología (Farmacia)
Microbiología (Farmacia)
Microbiología médica
32 Ciencias Médicas
3207.12 Parasitología
3201.03 Microbiología Clínica
title_short Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
title_full Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
title_fullStr Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
title_full_unstemmed Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
title_sort Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
dc.creator.none.fl_str_mv Ibáñez Escribano, Alexandra
Justo, Christine Aubrey C.
Skouridou, Vasso
Cools, Piet
Mulinganya, Guy
O´Sullivan, Ciara K.
author Ibáñez Escribano, Alexandra
author_facet Ibáñez Escribano, Alexandra
Justo, Christine Aubrey C.
Skouridou, Vasso
Cools, Piet
Mulinganya, Guy
O´Sullivan, Ciara K.
author_role author
author2 Justo, Christine Aubrey C.
Skouridou, Vasso
Cools, Piet
Mulinganya, Guy
O´Sullivan, Ciara K.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616.9
616.99
579.66
615.28
Ciencias Biomédicas
Enfermedades infecciosas
Parasitología (Farmacia)
Microbiología (Farmacia)
Microbiología médica
32 Ciencias Médicas
3207.12 Parasitología
3201.03 Microbiología Clínica
topic 616.9
616.99
579.66
615.28
Ciencias Biomédicas
Enfermedades infecciosas
Parasitología (Farmacia)
Microbiología (Farmacia)
Microbiología médica
32 Ciencias Médicas
3207.12 Parasitología
3201.03 Microbiología Clínica
description The development of low-cost, rapid point-of-care tests (POCT) for the detection of the parasite Trichomonas vaginalis is listed in the recent WHO global research priorities for sexually transmitted infections (STIs). Aiming to contribute to this call and aid in ending STIs as a public health threat, we report the development of a DNA aptamer-based POCT for T. vaginalis. Herein, we report an aptamer-based lateral flow assay (ALFA) based on dual aptamers for the detection of T. vaginalis. This is the first report of an ALFA that does not rely on biotin/streptavidin for either capture or reporter molecule. Aptamer capture and recognition relies on the use of an UV-crosslinked aminated capture aptamer on the nitrocellulose membrane and gold nanoparticles (AuNPs) functionalized with a thioctic acid-modified reporter aptamer. The developed ALFA has an estimated laboratory production cost of less than 1 € per test, with the running buffer and the ALFA strip stable for at least 1 year at room temperature (22 °C). The assay requires three simple operator steps from sample procurement to result with a 15-minute runtime. The developed ALFA can detect down to 1.6 × 105 T. vaginalis per mL and shows no cross-reactivity to common vaginal microorganisms and no matrix effect from clinical cervicovaginal lavage (CVL) samples is observed. Preliminary clinical evaluation using CVLs demonstrated that the assay has perfect concordance with wetmount assay on matching vaginal swabs. The developed rapid test offers a simple, stable, and low-cost diagnostic test for T. vaginalis.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/120130
url https://hdl.handle.net/20.500.14352/120130
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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