Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes

Cerium oxide nanoparticles (CeO NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whe...

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Autores: Carvajal, Silvia, Perramón, Meritxell|||0000-0002-1944-8337, Casals, Gregori|||0000-0002-3271-1371, Oró, Denise, Ribera Salas, Jordi|||0000-0002-0288-4220, Morales Ruiz, Manuel|||0000-0002-9074-2272, Casals, Eudald|||0000-0002-2900-7295, Casado, Pedro|||0000-0002-4207-9349, Melgar Lesmes, Pedro, Fernández-Varo, Guillermo|||0000-0002-7776-3300, Cutillas, Pedro, Puntes, Víctor|||0000-0001-8996-9499, Jiménez, Wladimiro|||0000-0002-9376-0214
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:223809
Acceso en línea:https://ddd.uab.cat/record/223809
https://dx.doi.org/urn:doi:10.3390/ijms20235959
Access Level:acceso abierto
Palabra clave:Cerium oxide nanoparticles
Oxidative stress
Human hepatic cells
Phosphoproteomics
NAFLD
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spelling Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytesCarvajal, SilviaPerramón, Meritxell|||0000-0002-1944-8337Casals, Gregori|||0000-0002-3271-1371Oró, DeniseRibera Salas, Jordi|||0000-0002-0288-4220Morales Ruiz, Manuel|||0000-0002-9074-2272Casals, Eudald|||0000-0002-2900-7295Casado, Pedro|||0000-0002-4207-9349Melgar Lesmes, PedroFernández-Varo, Guillermo|||0000-0002-7776-3300Cutillas, PedroPuntes, Víctor|||0000-0001-8996-9499Jiménez, Wladimiro|||0000-0002-9376-0214Cerium oxide nanoparticlesOxidative stressHuman hepatic cellsPhosphoproteomicsNAFLDCerium oxide nanoparticles (CeO NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO NPs did not modify HepG2 cell viability in basal conditions but reduced H O-and lipopolysaccharide (LPS)-induced cell death and prevented H O-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO NPs reverted the H O-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H O effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways. 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/223809https://dx.doi.org/urn:doi:10.3390/ijms20235959reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2015-64126-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI-15/00777Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-75358-RAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-094734-B-C21Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-219open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2238092026-06-06T12:50:31Z
dc.title.none.fl_str_mv Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
spellingShingle Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
Carvajal, Silvia
Cerium oxide nanoparticles
Oxidative stress
Human hepatic cells
Phosphoproteomics
NAFLD
title_short Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_full Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_fullStr Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_full_unstemmed Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_sort Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
dc.creator.none.fl_str_mv Carvajal, Silvia
Perramón, Meritxell|||0000-0002-1944-8337
Casals, Gregori|||0000-0002-3271-1371
Oró, Denise
Ribera Salas, Jordi|||0000-0002-0288-4220
Morales Ruiz, Manuel|||0000-0002-9074-2272
Casals, Eudald|||0000-0002-2900-7295
Casado, Pedro|||0000-0002-4207-9349
Melgar Lesmes, Pedro
Fernández-Varo, Guillermo|||0000-0002-7776-3300
Cutillas, Pedro
Puntes, Víctor|||0000-0001-8996-9499
Jiménez, Wladimiro|||0000-0002-9376-0214
author Carvajal, Silvia
author_facet Carvajal, Silvia
Perramón, Meritxell|||0000-0002-1944-8337
Casals, Gregori|||0000-0002-3271-1371
Oró, Denise
Ribera Salas, Jordi|||0000-0002-0288-4220
Morales Ruiz, Manuel|||0000-0002-9074-2272
Casals, Eudald|||0000-0002-2900-7295
Casado, Pedro|||0000-0002-4207-9349
Melgar Lesmes, Pedro
Fernández-Varo, Guillermo|||0000-0002-7776-3300
Cutillas, Pedro
Puntes, Víctor|||0000-0001-8996-9499
Jiménez, Wladimiro|||0000-0002-9376-0214
author_role author
author2 Perramón, Meritxell|||0000-0002-1944-8337
Casals, Gregori|||0000-0002-3271-1371
Oró, Denise
Ribera Salas, Jordi|||0000-0002-0288-4220
Morales Ruiz, Manuel|||0000-0002-9074-2272
Casals, Eudald|||0000-0002-2900-7295
Casado, Pedro|||0000-0002-4207-9349
Melgar Lesmes, Pedro
Fernández-Varo, Guillermo|||0000-0002-7776-3300
Cutillas, Pedro
Puntes, Víctor|||0000-0001-8996-9499
Jiménez, Wladimiro|||0000-0002-9376-0214
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerium oxide nanoparticles
Oxidative stress
Human hepatic cells
Phosphoproteomics
NAFLD
topic Cerium oxide nanoparticles
Oxidative stress
Human hepatic cells
Phosphoproteomics
NAFLD
description Cerium oxide nanoparticles (CeO NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO NPs did not modify HepG2 cell viability in basal conditions but reduced H O-and lipopolysaccharide (LPS)-induced cell death and prevented H O-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO NPs reverted the H O-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H O effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/223809
https://dx.doi.org/urn:doi:10.3390/ijms20235959
url https://ddd.uab.cat/record/223809
https://dx.doi.org/urn:doi:10.3390/ijms20235959
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2015-64126-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI-15/00777
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-75358-R
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-094734-B-C21
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-219
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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