Biocompatible antimicrobial electrospun nanofibers functionalized with epsilon-poly-L-lysine
The antimicrobial polypeptide epsilon-poly(L-lysine) (epsilon-PL) was electrostatically incorporated to poly(acrylic acid) (PAA)/poly(vinyl alcohol) (PVA) electrospun nanofibers. epsilon-PL loading and distribution was assessed by infrared spectra, zeta-potential measurements and the primary amino r...
| Autores: | , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/64563 |
| Acesso em linha: | http://hdl.handle.net/10017/64563 https://dx.doi.org/10.1016/j.ijpharm.2018.10.037 |
| Access Level: | acceso abierto |
| Palavra-chave: | Electrospun fibers epsilon-Poly(L-lysine) Antibacterial materials Biocompatible dressings Química Chemistry |
| Resumo: | The antimicrobial polypeptide epsilon-poly(L-lysine) (epsilon-PL) was electrostatically incorporated to poly(acrylic acid) (PAA)/poly(vinyl alcohol) (PVA) electrospun nanofibers. epsilon-PL loading and distribution was assessed by infrared spectra, zeta-potential measurements and the primary amino reactive dye fluorescamine. Functionalized fibers with 485 +/- 140 nm diameter, could be loaded with 0.57-0.74 g epsilon-PL (g dressing)(-1) that released at a constant rate of 5.4 +/- 2.8 mg epsilon-PL (g dressing day)(-1). Such a dressings resulted in two orders of magnitude lower bacterial colonization than non-functionalized PAA-PVA after 14 days of incubation. Bacterial impairment was attributed to the damage of cell membranes and the formation of intracellular reactive oxygen species. epsilon-PL functionalized nanofibers did not display cytotoxicity to human corneal epithelial cells, HCEpC, in 24 h MTT assays. However, the viability of rapidly growing tumoral HeLa cells decreased > 50% under the same conditions. The prepared biocompatible nanofibrous dressings with durable antibacterial activity show potential application as wound dressings and other biomedical uses. |
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