Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development

[EN]Hormone patterns tailor cell fate decisions during plant organ formation. Among them, auxins and cytokinins are critical phytohormones during early development. Nitric oxide (NO) modulates root architecture by the control of auxin spatial patterns. However, NO involvement during the coordination...

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Detalhes bibliográficos
Autores: Sánchez Vicente, María Inmaculada, Lechón Gómez, Tamara, Fernández Marcos, María, Sanz Andreu, Luis, Lorenzo Sánchez, Óscar
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Universidad de Salamanca (USAL)
Repositório:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/157109
Acesso em linha:http://hdl.handle.net/10366/157109
Access Level:Acceso aberto
Palavra-chave:Auxin response
Auxin transport
Leaf morphology
Nitric oxide homeostasis mutants
PIN-FORMED 1
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spelling Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot developmentSánchez Vicente, María InmaculadaLechón Gómez, TamaraFernández Marcos, MaríaSanz Andreu, LuisLorenzo Sánchez, ÓscarAuxin responseAuxin transportLeaf morphologyNitric oxide homeostasis mutantsPIN-FORMED 1[EN]Hormone patterns tailor cell fate decisions during plant organ formation. Among them, auxins and cytokinins are critical phytohormones during early development. Nitric oxide (NO) modulates root architecture by the control of auxin spatial patterns. However, NO involvement during the coordination of shoot organogenesis remains unclear. Here, we explore the effect of NO during shoot development by using a phenotypic, cellular, and genetic analysis in Arabidopsis thaliana and get new insights into the characterization of NO-mediated leaf-related phenotypes. NO homeostasis mutants are impaired in several shoot architectural parameters, including phyllotactic patterns, inflorescence stem elongation, silique production, leaf number, and margin. Auxin distribution is a key feature for tissue differentiation and need to be controlled at different levels (i.e., synthesis, transport, and degradation mechanisms). The phenotypes resulting from the introduction of the cue1 mutation in the axr1 auxin resistant and pin1 backgrounds exacerbate the relationship between NO and auxins. Using the auxin reporter DR5:GUS, we observed an increase in auxin maxima under NO-deficient mutant backgrounds and NO scavenging, pointing to NO-ASSOCIATED 1 (NOA1) as the main player related to NO production in this process. Furthermore, polar auxin transport is mainly regulated by PIN-FORMED 1 (PIN1), which controls the flow along leaf margin and venations. Analysis of PIN1 protein levels shows that NO controls its accumulation during leaf development, impacting the auxin mediated mechanism of leaf building. With these findings, we also provide evidence for the NO opposite effects to determine root and shoot architecture, in terms of PIN1 accumulation under NO overproduction.Frontiers Media202420242021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/157109reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésBIO2015-68957-REDTRED2018-102397-TAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1571092026-06-07T06:28:51Z
dc.title.none.fl_str_mv Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
title Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
spellingShingle Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
Sánchez Vicente, María Inmaculada
Auxin response
Auxin transport
Leaf morphology
Nitric oxide homeostasis mutants
PIN-FORMED 1
title_short Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
title_full Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
title_fullStr Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
title_full_unstemmed Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
title_sort Nitric oxide alters the pattern of auxin maxima and PIN-FORMED1 during shoot development
dc.creator.none.fl_str_mv Sánchez Vicente, María Inmaculada
Lechón Gómez, Tamara
Fernández Marcos, María
Sanz Andreu, Luis
Lorenzo Sánchez, Óscar
author Sánchez Vicente, María Inmaculada
author_facet Sánchez Vicente, María Inmaculada
Lechón Gómez, Tamara
Fernández Marcos, María
Sanz Andreu, Luis
Lorenzo Sánchez, Óscar
author_role author
author2 Lechón Gómez, Tamara
Fernández Marcos, María
Sanz Andreu, Luis
Lorenzo Sánchez, Óscar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Auxin response
Auxin transport
Leaf morphology
Nitric oxide homeostasis mutants
PIN-FORMED 1
topic Auxin response
Auxin transport
Leaf morphology
Nitric oxide homeostasis mutants
PIN-FORMED 1
description [EN]Hormone patterns tailor cell fate decisions during plant organ formation. Among them, auxins and cytokinins are critical phytohormones during early development. Nitric oxide (NO) modulates root architecture by the control of auxin spatial patterns. However, NO involvement during the coordination of shoot organogenesis remains unclear. Here, we explore the effect of NO during shoot development by using a phenotypic, cellular, and genetic analysis in Arabidopsis thaliana and get new insights into the characterization of NO-mediated leaf-related phenotypes. NO homeostasis mutants are impaired in several shoot architectural parameters, including phyllotactic patterns, inflorescence stem elongation, silique production, leaf number, and margin. Auxin distribution is a key feature for tissue differentiation and need to be controlled at different levels (i.e., synthesis, transport, and degradation mechanisms). The phenotypes resulting from the introduction of the cue1 mutation in the axr1 auxin resistant and pin1 backgrounds exacerbate the relationship between NO and auxins. Using the auxin reporter DR5:GUS, we observed an increase in auxin maxima under NO-deficient mutant backgrounds and NO scavenging, pointing to NO-ASSOCIATED 1 (NOA1) as the main player related to NO production in this process. Furthermore, polar auxin transport is mainly regulated by PIN-FORMED 1 (PIN1), which controls the flow along leaf margin and venations. Analysis of PIN1 protein levels shows that NO controls its accumulation during leaf development, impacting the auxin mediated mechanism of leaf building. With these findings, we also provide evidence for the NO opposite effects to determine root and shoot architecture, in terms of PIN1 accumulation under NO overproduction.
publishDate 2021
dc.date.none.fl_str_mv 2021
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/157109
url http://hdl.handle.net/10366/157109
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BIO2015-68957-REDT
RED2018-102397-T
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.228081

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