Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast

When DNA replication is challenged, cells activate a DNA synthesis checkpoint blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M phase entry, stabilizing stalled replication forks and...

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Detalhes bibliográficos
Autor: Gómez Escoda, Blanca
Tipo de documento: tese
Estado:Versão publicada
Data de publicação:2010
País:España
Recursos:CBUC, CESCA
Repositório:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/7223
Acesso em linha:http://www.tdx.cat/TDX-0207111-150509
http://hdl.handle.net/10803/7223
Access Level:Acceso aberto
Palavra-chave:stress
kinase
phosphorylation
DNA damage
Checkpoint
Cell Cycle
transcription factor
transcription
replication
yeast
estrés
kinasa
fosforilación
daño en DNA
checkpoint
ciclo celular
factor de transcripción
transcripción
replicación
Schizosaccharomyces pombe
levadura
577
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oai_identifier_str oai:www.tdx.cat:10803/7223
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repository_id_str
spelling Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeastGómez Escoda, BlancastresskinasephosphorylationDNA damageCheckpointCell Cycletranscription factortranscriptionreplicationyeastestréskinasafosforilacióndaño en DNAcheckpointciclo celularfactor de transcripcióntranscripciónreplicaciónSchizosaccharomyces pombelevadura577When DNA replication is challenged, cells activate a DNA synthesis checkpoint blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M phase entry, stabilizing stalled replication forks and triggering transcriptional activation of S-phase genes; the molecular basis of this last effect remains largely unknown. The MBF complex controls the transcription of S-phase genes. We have purified novel interactors of the MBF complex and among them we have identified the repressor Max1. When the DNA synthesis checkpoint is activated, Max1 is phosphorylated by Cds1 resulting in the abrogation of its binding to MBF. As a consequence, MBF-dependent transcription is maintained active until cells are able to overcome this challenge.Cuando la replicación del DNA se ve alterada, las células activan un mecanismo de control bloqueando la progresión del ciclo celular hasta que son capaces de superar el daño. En la levadura de fisión, Cds1 es la proteína kinasa efectora de dicha respuesta, mediante inhibición de la entrada en fase M, estabilización las horquillas de replicación bloqueadas, e inducción de la activación de la transcripción de los genes de fase S; siendo la base molecular de este último proceso poco conocida. El factor de transcripción MBF controla la transcripción de los genes de fase S. Hemos purificado proteínas que interaccionan con MBF, y entre ellas, hemos identificado al represor Max1. Cuando el checkpoint de síntesis de DNA es activado, Max1 es fosforilado por la kinasa Cds1, y esto se traduce en la disociación de Max1 del complejo MBF. Como consecuencia, la transcripción MBF-dependiente se mantiene activa hasta que las células son capaces de superar el daño.Programa de doctorat en BiomedicinaUniversitat Pompeu FabraAyté del Olmo, JoséUniversitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut2011201120102011info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.tdx.cat/TDX-0207111-150509http://hdl.handle.net/10803/7223TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/72232026-06-14T12:46:07Z
dc.title.none.fl_str_mv Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
title Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
spellingShingle Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
Gómez Escoda, Blanca
stress
kinase
phosphorylation
DNA damage
Checkpoint
Cell Cycle
transcription factor
transcription
replication
yeast
estrés
kinasa
fosforilación
daño en DNA
checkpoint
ciclo celular
factor de transcripción
transcripción
replicación
Schizosaccharomyces pombe
levadura
577
title_short Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
title_full Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
title_fullStr Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
title_full_unstemmed Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
title_sort Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
dc.creator.none.fl_str_mv Gómez Escoda, Blanca
author Gómez Escoda, Blanca
author_facet Gómez Escoda, Blanca
author_role author
dc.contributor.none.fl_str_mv Ayté del Olmo, José
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
dc.subject.none.fl_str_mv stress
kinase
phosphorylation
DNA damage
Checkpoint
Cell Cycle
transcription factor
transcription
replication
yeast
estrés
kinasa
fosforilación
daño en DNA
checkpoint
ciclo celular
factor de transcripción
transcripción
replicación
Schizosaccharomyces pombe
levadura
577
topic stress
kinase
phosphorylation
DNA damage
Checkpoint
Cell Cycle
transcription factor
transcription
replication
yeast
estrés
kinasa
fosforilación
daño en DNA
checkpoint
ciclo celular
factor de transcripción
transcripción
replicación
Schizosaccharomyces pombe
levadura
577
description When DNA replication is challenged, cells activate a DNA synthesis checkpoint blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M phase entry, stabilizing stalled replication forks and triggering transcriptional activation of S-phase genes; the molecular basis of this last effect remains largely unknown. The MBF complex controls the transcription of S-phase genes. We have purified novel interactors of the MBF complex and among them we have identified the repressor Max1. When the DNA synthesis checkpoint is activated, Max1 is phosphorylated by Cds1 resulting in the abrogation of its binding to MBF. As a consequence, MBF-dependent transcription is maintained active until cells are able to overcome this challenge.
publishDate 2010
dc.date.none.fl_str_mv 2010
2011
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.tdx.cat/TDX-0207111-150509
http://hdl.handle.net/10803/7223
url http://www.tdx.cat/TDX-0207111-150509
http://hdl.handle.net/10803/7223
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403896442519552
score 15,198674

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