Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats
Rotavirus (RV) is considered to be the most common cause of gastroenteritis among infants aged less than 5 years old. Human milk bioactive compounds have the ability to modulate the diarrheic process caused by several intestinal pathogens. This study aimed to evaluate the potential protective role o...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/130526 |
| Acceso en línea: | https://hdl.handle.net/2445/130526 |
| Access Level: | acceso abierto |
| Palabra clave: | Prebiòtics Diarrea Llet materna Malalties intestinals Prebiotics Diarrhea Breast milk Intestinal diseases |
| id |
ES_1612b44d60757a9d64273c8a3a63b675 |
|---|---|
| oai_identifier_str |
oai:diposit.ub.edu:2445/130526 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling RatsAzagra Boronat, IgnasiMassot Cladera, MalenKnipping, K.van't Land, B.Stahl, B.Garssen, J.Rodríguez Lagunas, María JoséFranch i Masferrer, ÀngelsCastell, MargaridaPérez-Cano, Francisco J.PrebiòticsDiarreaLlet maternaMalalties intestinalsPrebioticsDiarrheaBreast milkIntestinal diseasesRotavirus (RV) is considered to be the most common cause of gastroenteritis among infants aged less than 5 years old. Human milk bioactive compounds have the ability to modulate the diarrheic process caused by several intestinal pathogens. This study aimed to evaluate the potential protective role of a specific human milk oligosaccharide, 2′-fucosyllactose (2′-FL), a mixture of the prebiotic short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides 9:1 (GOS/FOS) and their combination (2′-FL+GOS/FOS) on RV-induced diarrhea in suckling rats. The nutritional intervention was performed from the second to the sixteenth day of life by oral gavage and on day 5 an RV strain was orally administered to induce infection. Fecal samples were scored daily to assess the clinical pattern of severity, incidence and duration of diarrhea. Blood and tissues were obtained at day 8 and 16 in order to evaluate the effects on the epithelial barrier and the mucosal and systemic immune responses. In the assessment of severity, incidence and duration of diarrhea, both 2′-FL and GOS/FOS displayed a beneficial effect in terms of amelioration. However, the mechanisms involved seemed to differ: 2′ -FL displayed a direct ability to promote intestinal maturation and to enhance neonatal immune responses, while GOS/FOS induced an intestinal trophic effect and an RV-blocking action. The combination of 2′-FL and GOS/FOS showed additive effects in some variables. Therefore, it could be a good strategy to add these compounds in combination to infant formulas, to protect against human RV-induced diarrhea in children. Keywords: 2′-FL, scGOS/lcFOS, prebiotic, rotavirus, diarrhea, suckling ratsFrontiers Media2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/130526Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fcimb.2018.00372Frontiers in Cellular and Infection Microbiology, 2018, vol. 23, num. 8, p. 1-17https://doi.org/10.3389/fcimb.2018.00372cc-by (c) Azagra Boronat, Ignasi et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1305262026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| title |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| spellingShingle |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats Azagra Boronat, Ignasi Prebiòtics Diarrea Llet materna Malalties intestinals Prebiotics Diarrhea Breast milk Intestinal diseases |
| title_short |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| title_full |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| title_fullStr |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| title_full_unstemmed |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| title_sort |
Supplementation With 2′-FL and scGOS/lcFOS Ameliorates Rotavirus-Induced Diarrhea in Suckling Rats |
| dc.creator.none.fl_str_mv |
Azagra Boronat, Ignasi Massot Cladera, Malen Knipping, K. van't Land, B. Stahl, B. Garssen, J. Rodríguez Lagunas, María José Franch i Masferrer, Àngels Castell, Margarida Pérez-Cano, Francisco J. |
| author |
Azagra Boronat, Ignasi |
| author_facet |
Azagra Boronat, Ignasi Massot Cladera, Malen Knipping, K. van't Land, B. Stahl, B. Garssen, J. Rodríguez Lagunas, María José Franch i Masferrer, Àngels Castell, Margarida Pérez-Cano, Francisco J. |
| author_role |
author |
| author2 |
Massot Cladera, Malen Knipping, K. van't Land, B. Stahl, B. Garssen, J. Rodríguez Lagunas, María José Franch i Masferrer, Àngels Castell, Margarida Pérez-Cano, Francisco J. |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Prebiòtics Diarrea Llet materna Malalties intestinals Prebiotics Diarrhea Breast milk Intestinal diseases |
| topic |
Prebiòtics Diarrea Llet materna Malalties intestinals Prebiotics Diarrhea Breast milk Intestinal diseases |
| description |
Rotavirus (RV) is considered to be the most common cause of gastroenteritis among infants aged less than 5 years old. Human milk bioactive compounds have the ability to modulate the diarrheic process caused by several intestinal pathogens. This study aimed to evaluate the potential protective role of a specific human milk oligosaccharide, 2′-fucosyllactose (2′-FL), a mixture of the prebiotic short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides 9:1 (GOS/FOS) and their combination (2′-FL+GOS/FOS) on RV-induced diarrhea in suckling rats. The nutritional intervention was performed from the second to the sixteenth day of life by oral gavage and on day 5 an RV strain was orally administered to induce infection. Fecal samples were scored daily to assess the clinical pattern of severity, incidence and duration of diarrhea. Blood and tissues were obtained at day 8 and 16 in order to evaluate the effects on the epithelial barrier and the mucosal and systemic immune responses. In the assessment of severity, incidence and duration of diarrhea, both 2′-FL and GOS/FOS displayed a beneficial effect in terms of amelioration. However, the mechanisms involved seemed to differ: 2′ -FL displayed a direct ability to promote intestinal maturation and to enhance neonatal immune responses, while GOS/FOS induced an intestinal trophic effect and an RV-blocking action. The combination of 2′-FL and GOS/FOS showed additive effects in some variables. Therefore, it could be a good strategy to add these compounds in combination to infant formulas, to protect against human RV-induced diarrhea in children. Keywords: 2′-FL, scGOS/lcFOS, prebiotic, rotavirus, diarrhea, suckling rats |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/130526 |
| url |
https://hdl.handle.net/2445/130526 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.3389/fcimb.2018.00372 Frontiers in Cellular and Infection Microbiology, 2018, vol. 23, num. 8, p. 1-17 https://doi.org/10.3389/fcimb.2018.00372 |
| dc.rights.none.fl_str_mv |
cc-by (c) Azagra Boronat, Ignasi et al., 2018 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Azagra Boronat, Ignasi et al., 2018 http://creativecommons.org/licenses/by/3.0/es |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Bioquímica i Fisiologia) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
| instname_str |
Universidad de Barcelona |
| reponame_str |
Dipòsit Digital de la UB |
| collection |
Dipòsit Digital de la UB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869403834482163712 |
| score |
15.228081 |