Changes in size of European hake larvae and conversion factors for different preservation mediums

The objective of this study was to determine how preservation methods affect the standard length (SL, mm) of European hake larvae. We used five fixative methods: (i) freezing in seawater at −20°C; (ii) RNAlater at −80°C; (iii) 4% borax-buffered formalin; (iv) freezing with liquid nitrogen and (v) 95...

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Detalles Bibliográficos
Autores: Rodríguez-Fernández, Lorena, Leal-Ramos, Ana María, Lago-Rouco, María Jesús, Laiz-Carrión, Raúl, Piñeiro-Álvarez, Carmen Gloria
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/326490
Acceso en línea:http://hdl.handle.net/10261/326490
Access Level:acceso abierto
Palabra clave:Early larval stages
Pesquerías
Centro Oceanográfico de Málaga
European hake
Merluccius merluccius
preservation
shrinkage
size conversion
fish
weight
ecology
size
mortality
Hake fisheries
AQUACUL
id ES_156df634bb2c8528ab48b97e8e5d6cff
oai_identifier_str oai:digital.csic.es:10261/326490
network_acronym_str ES
network_name_str España
repository_id_str
spelling Changes in size of European hake larvae and conversion factors for different preservation mediumsRodríguez-Fernández, LorenaLeal-Ramos, Ana MaríaLago-Rouco, María JesúsLaiz-Carrión, RaúlPiñeiro-Álvarez, Carmen GloriaEarly larval stagesPesqueríasCentro Oceanográfico de MálagaEuropean hakeMerluccius merlucciuspreservationshrinkagesize conversionfishweightecologysizemortalityHake fisheriesAQUACULThe objective of this study was to determine how preservation methods affect the standard length (SL, mm) of European hake larvae. We used five fixative methods: (i) freezing in seawater at −20°C; (ii) RNAlater at −80°C; (iii) 4% borax-buffered formalin; (iv) freezing with liquid nitrogen and (v) 95% ethanol. Standard length hake larvae were initially measured and individually fixed in each of the five fixatives. To evaluate the effect of the time preserved in each one, we obtained the SL of larvae for a second time after three different preservation periods: 15, 44 and 91 days. To assess the effect of initial size on final shrinkage, we analysed two groups of larvae born in captivity, 15 and 24 days post hatching respectively. Most shrinkage occurred within the first period of preservation in all fixatives, the preservation time having no effect on the percentage of shrinkage for any preservative. However, initial SL affects the percentage of shrinkage depending on the fixative used. We suggest five correlation equations to convert an initial SL into a final contracted size of hake larvae for each fixative.CRAMEREcología del reclutamiento de la merluza: implicaciones sobre la gestión del recursoECOPREGAEcología del reclutamiento y primeros estadios de vida de la merluza en GaliciaSI202320232021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/326490reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Málagainfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3264902026-05-22T06:33:51Z
dc.title.none.fl_str_mv Changes in size of European hake larvae and conversion factors for different preservation mediums
title Changes in size of European hake larvae and conversion factors for different preservation mediums
spellingShingle Changes in size of European hake larvae and conversion factors for different preservation mediums
Rodríguez-Fernández, Lorena
Early larval stages
Pesquerías
Centro Oceanográfico de Málaga
European hake
Merluccius merluccius
preservation
shrinkage
size conversion
fish
weight
ecology
size
mortality
Hake fisheries
AQUACUL
title_short Changes in size of European hake larvae and conversion factors for different preservation mediums
title_full Changes in size of European hake larvae and conversion factors for different preservation mediums
title_fullStr Changes in size of European hake larvae and conversion factors for different preservation mediums
title_full_unstemmed Changes in size of European hake larvae and conversion factors for different preservation mediums
title_sort Changes in size of European hake larvae and conversion factors for different preservation mediums
dc.creator.none.fl_str_mv Rodríguez-Fernández, Lorena
Leal-Ramos, Ana María
Lago-Rouco, María Jesús
Laiz-Carrión, Raúl
Piñeiro-Álvarez, Carmen Gloria
author Rodríguez-Fernández, Lorena
author_facet Rodríguez-Fernández, Lorena
Leal-Ramos, Ana María
Lago-Rouco, María Jesús
Laiz-Carrión, Raúl
Piñeiro-Álvarez, Carmen Gloria
author_role author
author2 Leal-Ramos, Ana María
Lago-Rouco, María Jesús
Laiz-Carrión, Raúl
Piñeiro-Álvarez, Carmen Gloria
author2_role author
author
author
author
dc.subject.none.fl_str_mv Early larval stages
Pesquerías
Centro Oceanográfico de Málaga
European hake
Merluccius merluccius
preservation
shrinkage
size conversion
fish
weight
ecology
size
mortality
Hake fisheries
AQUACUL
topic Early larval stages
Pesquerías
Centro Oceanográfico de Málaga
European hake
Merluccius merluccius
preservation
shrinkage
size conversion
fish
weight
ecology
size
mortality
Hake fisheries
AQUACUL
description The objective of this study was to determine how preservation methods affect the standard length (SL, mm) of European hake larvae. We used five fixative methods: (i) freezing in seawater at −20°C; (ii) RNAlater at −80°C; (iii) 4% borax-buffered formalin; (iv) freezing with liquid nitrogen and (v) 95% ethanol. Standard length hake larvae were initially measured and individually fixed in each of the five fixatives. To evaluate the effect of the time preserved in each one, we obtained the SL of larvae for a second time after three different preservation periods: 15, 44 and 91 days. To assess the effect of initial size on final shrinkage, we analysed two groups of larvae born in captivity, 15 and 24 days post hatching respectively. Most shrinkage occurred within the first period of preservation in all fixatives, the preservation time having no effect on the percentage of shrinkage for any preservative. However, initial SL affects the percentage of shrinkage depending on the fixative used. We suggest five correlation equations to convert an initial SL into a final contracted size of hake larvae for each fixative.
publishDate 2021
dc.date.none.fl_str_mv 2021
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/326490
url http://hdl.handle.net/10261/326490
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Málaga
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403795441582080
score 15,228081

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