Routine quantification of phytoplankton groups - microscopy or pigment analyses?

12 pages, 5 figures, 1 table.-- It is ELOISE contribution no. 448/40

Detalles Bibliográficos
Autores: Havskum, Harry, Schlüter, Louise, Scharek, Renate, Berdalet, Elisa, Jacquet, Stéphan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/135008
Acceso en línea:http://hdl.handle.net/10261/135008
Access Level:acceso abierto
Palabra clave:HPLC pigment analyses
CHEMTAX program
Microscopy
Chlorophyllide a
Chlorophylla a carbon ratios
Pigmented flagellates
Cyanobacteria
Diatoms
CHEMTAX
HPLC
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spelling Routine quantification of phytoplankton groups - microscopy or pigment analyses?Havskum, HarrySchlüter, LouiseScharek, RenateBerdalet, ElisaJacquet, StéphanHPLC pigment analysesCHEMTAX programMicroscopyChlorophyllide aChlorophylla a carbon ratiosPigmented flagellatesCyanobacteriaDiatomsCHEMTAXHPLC12 pages, 5 figures, 1 table.-- It is ELOISE contribution no. 448/40Phytoplankton pigments in samples taken from nutrient-enriched and non-enriched 3 m3 seawater enclosures were separated and quantified using high-performance liquid chromatography (HPLC). The enclosures were with and without inorganic (N, P, Si) and organic (glucose, C) nutrient enrichments, resulting in a variation of phytoplankton groups in time and space. The relative contribution of the major phytoplankton groups to the total chlorophyll a (i.e. chlorophyll a plus chlorophyllide a) was estimated by the CHEMTAX program. The results were compared to phytoplankton groups identified and quantified by light and epifluorescence microscopy. For the pigmented flagellate groups the results obtained by microscopy and pigment analyses using the CHEMTAX program showed similar trends. The picocyanobacteria were readily quantified by microscopy and the results were similar to those obtained by flow-cytometry, while the CHEMTAX program for the cyanobacteria revealed different trends. Microscopy and pigment analyses provided similar trends in diatom population development. Estimated diatom contributions to total phytoplankton biomass, however, were considerably higher when based on microscopy than when based on the CHEMTAX program, especially in Si-amended enclosures. Total chlorophyll a:carbon ratios for diatoms were at the lower end of a previously reported range between 1:27 and 1:67. For the pigmented flagellate groups the total chlorophyll a:carbon ratios were above that range. In routine monitoring of phytoplankton we recommend the use of the CHEMTAX program based on HPLC pigment analyses accompanied by a screening for the dominating species by microscopy, and by flow-cytometry for quantification of picocyanobacteriaThis study was supported by MAST III (contracts MAS3-CT-0016-MEDEA, and MAS3-CT96-5034), by a French doctoral fellowship from the Ministère de la Recherche et de l’Enseignement Supérieur, and by the research project NTAP (contract EVK3-CT-2000-00022) of the EU RTD Programme ‘Environment and Sustainable Development’ that forms part of the ELOISE projects clusterPeer ReviewedInter ResearchConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620042016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/135008reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3354/meps273031Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1350082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Routine quantification of phytoplankton groups - microscopy or pigment analyses?
title Routine quantification of phytoplankton groups - microscopy or pigment analyses?
spellingShingle Routine quantification of phytoplankton groups - microscopy or pigment analyses?
Havskum, Harry
HPLC pigment analyses
CHEMTAX program
Microscopy
Chlorophyllide a
Chlorophylla a carbon ratios
Pigmented flagellates
Cyanobacteria
Diatoms
CHEMTAX
HPLC
title_short Routine quantification of phytoplankton groups - microscopy or pigment analyses?
title_full Routine quantification of phytoplankton groups - microscopy or pigment analyses?
title_fullStr Routine quantification of phytoplankton groups - microscopy or pigment analyses?
title_full_unstemmed Routine quantification of phytoplankton groups - microscopy or pigment analyses?
title_sort Routine quantification of phytoplankton groups - microscopy or pigment analyses?
dc.creator.none.fl_str_mv Havskum, Harry
Schlüter, Louise
Scharek, Renate
Berdalet, Elisa
Jacquet, Stéphan
author Havskum, Harry
author_facet Havskum, Harry
Schlüter, Louise
Scharek, Renate
Berdalet, Elisa
Jacquet, Stéphan
author_role author
author2 Schlüter, Louise
Scharek, Renate
Berdalet, Elisa
Jacquet, Stéphan
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv HPLC pigment analyses
CHEMTAX program
Microscopy
Chlorophyllide a
Chlorophylla a carbon ratios
Pigmented flagellates
Cyanobacteria
Diatoms
CHEMTAX
HPLC
topic HPLC pigment analyses
CHEMTAX program
Microscopy
Chlorophyllide a
Chlorophylla a carbon ratios
Pigmented flagellates
Cyanobacteria
Diatoms
CHEMTAX
HPLC
description 12 pages, 5 figures, 1 table.-- It is ELOISE contribution no. 448/40
publishDate 2004
dc.date.none.fl_str_mv 2004
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/135008
url http://hdl.handle.net/10261/135008
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3354/meps273031
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Inter Research
publisher.none.fl_str_mv Inter Research
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403790151516160
score 15,198674

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