The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression

Epsilon toxin (Etx) from Clostridium perfringens is a pore-forming protein that crosses the blood-brain barrier, binds to myelin, and, hence, has been suggested to be a putative agent for the onset of multiple sclerosis, a demyelinating neuroinflammatory disease. Recently, myelin and lymphocyte (MAL...

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Autores: Blanch Lozano, Marta, Dorca Arévalo, Jonatan, Not, Anna, Cases, Mercè, Gómez de Aranda Pulgarín, Inmaculada, Martínez Yélamos, Antonio, Martínez Yélamos, Sergio, Solsona Sancho, Carles, Blasi Cabús, Joan
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/128148
Acceso en línea:https://hdl.handle.net/2445/128148
Access Level:acceso abierto
Palabra clave:Clostridi
Cèl·lules T
Esclerosi múltiple
Malalties autoimmunitàries
Clostridium
T cells
Multiple sclerosis
Autoimmune diseases
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spelling The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expressionBlanch Lozano, MartaDorca Arévalo, JonatanNot, AnnaCases, MercèGómez de Aranda Pulgarín, InmaculadaMartínez Yélamos, AntonioMartínez Yélamos, SergioSolsona Sancho, CarlesBlasi Cabús, JoanClostridiCèl·lules TEsclerosi múltipleMalalties autoimmunitàriesClostridiumT cellsMultiple sclerosisAutoimmune diseasesEpsilon toxin (Etx) from Clostridium perfringens is a pore-forming protein that crosses the blood-brain barrier, binds to myelin, and, hence, has been suggested to be a putative agent for the onset of multiple sclerosis, a demyelinating neuroinflammatory disease. Recently, myelin and lymphocyte (MAL) protein has been identified to be a key protein in the cytotoxic effect of Etx; however, the association of Etx with the immune system remains a central question. Here, we show that Etx selectively recognizes and kills only human cell lines expressing MAL protein through a direct Etx-MAL protein interaction. Experiments on lymphocytic cell lines revealed that MAL protein-expressing T cells, but not B cells, are sensitive to Etx and reveal that the toxin may be used as a molecular tool to distinguish subpopulations of lymphocytes. The overall results open the door to investigation of the role of Etx and Clostridium perfringens on inflammatory and autoimmune diseases like multiple sclerosis.American Society for Microbiology2019201920182019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion18 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/128148Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1128/MCB.00086-18Molecular and Cellular Biology, 2018, vol. 38, num. 19, p. 00086-18https://doi.org/10.1128/MCB.00086-18(c) American Society for Microbiology, 2018info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1281482026-05-29T05:05:01Z
dc.title.none.fl_str_mv The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
title The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
spellingShingle The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
Blanch Lozano, Marta
Clostridi
Cèl·lules T
Esclerosi múltiple
Malalties autoimmunitàries
Clostridium
T cells
Multiple sclerosis
Autoimmune diseases
title_short The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
title_full The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
title_fullStr The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
title_full_unstemmed The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
title_sort The Cytotoxicity of Epsilon toxin from Clostridium perfringens on lymphocytes is mediated by MAL protein expression
dc.creator.none.fl_str_mv Blanch Lozano, Marta
Dorca Arévalo, Jonatan
Not, Anna
Cases, Mercè
Gómez de Aranda Pulgarín, Inmaculada
Martínez Yélamos, Antonio
Martínez Yélamos, Sergio
Solsona Sancho, Carles
Blasi Cabús, Joan
author Blanch Lozano, Marta
author_facet Blanch Lozano, Marta
Dorca Arévalo, Jonatan
Not, Anna
Cases, Mercè
Gómez de Aranda Pulgarín, Inmaculada
Martínez Yélamos, Antonio
Martínez Yélamos, Sergio
Solsona Sancho, Carles
Blasi Cabús, Joan
author_role author
author2 Dorca Arévalo, Jonatan
Not, Anna
Cases, Mercè
Gómez de Aranda Pulgarín, Inmaculada
Martínez Yélamos, Antonio
Martínez Yélamos, Sergio
Solsona Sancho, Carles
Blasi Cabús, Joan
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Clostridi
Cèl·lules T
Esclerosi múltiple
Malalties autoimmunitàries
Clostridium
T cells
Multiple sclerosis
Autoimmune diseases
topic Clostridi
Cèl·lules T
Esclerosi múltiple
Malalties autoimmunitàries
Clostridium
T cells
Multiple sclerosis
Autoimmune diseases
description Epsilon toxin (Etx) from Clostridium perfringens is a pore-forming protein that crosses the blood-brain barrier, binds to myelin, and, hence, has been suggested to be a putative agent for the onset of multiple sclerosis, a demyelinating neuroinflammatory disease. Recently, myelin and lymphocyte (MAL) protein has been identified to be a key protein in the cytotoxic effect of Etx; however, the association of Etx with the immune system remains a central question. Here, we show that Etx selectively recognizes and kills only human cell lines expressing MAL protein through a direct Etx-MAL protein interaction. Experiments on lymphocytic cell lines revealed that MAL protein-expressing T cells, but not B cells, are sensitive to Etx and reveal that the toxin may be used as a molecular tool to distinguish subpopulations of lymphocytes. The overall results open the door to investigation of the role of Etx and Clostridium perfringens on inflammatory and autoimmune diseases like multiple sclerosis.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/128148
url https://hdl.handle.net/2445/128148
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1128/MCB.00086-18
Molecular and Cellular Biology, 2018, vol. 38, num. 19, p. 00086-18
https://doi.org/10.1128/MCB.00086-18
dc.rights.none.fl_str_mv (c) American Society for Microbiology, 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Microbiology, 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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