Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico

A Gram-negative, short rod to oval-shaped bacterium (strain MW2aT) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2aT was moderately halophilic, growing at NaCl concentrations in the range 0-25% (w/v) with opt...

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Autores: Soto Ramírez, Nelís, Sánchez-Porro Álvarez, Cristina, Rosas, Soniris, González, Wildaomaris, Quiñones, Marian, Ventosa Ucero, Antonio, Montalvo Rodríguez, Rafael
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2007
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/165221
Acceso en línea:https://hdl.handle.net/11441/165221
https://doi.org/10.1099/ijs.0.64818-0
Access Level:acceso abierto
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spelling Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto RicoSoto Ramírez, NelísSánchez-Porro Álvarez, CristinaRosas, SonirisGonzález, WildaomarisQuiñones, MarianVentosa Ucero, AntonioMontalvo Rodríguez, RafaelA Gram-negative, short rod to oval-shaped bacterium (strain MW2aT) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2aT was moderately halophilic, growing at NaCl concentrations in the range 0-25% (w/v) with optimum growth at 5% (w/v) NaCl. Growth occurred at 12-40°C (optimum, 30-35°C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Strain MW2aT was strictly aerobic. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Halomonas. The closest relative was Halomonas marisflavi, with 98.6% 16S rRNA gene sequence similarity. The DNA G + C content of strain MW2aT was 61.5 mol%, which is in the range of values for Halomonas species. DNA-DNA hybridization with H. marisflavi showed a relatedness of 42% and lower values were obtained with respect to other related Halomonas species. The major fatty acids were C16:0, C19:0 cyclo ω8c, C18:1 ω7c and C12:0 3-OH. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain Nw2aT represents a novel species within the genus Halomonas. The name Halomonas avicenniae sp. nov. is proposed, with strain MW2aT (= CECT 7193T =CCM 7396T) as the type strain.European Union QLK3-CT-2002-01972Ministerio de Educación y Ciencia BMC2003-01344Microbiology SocietyMicrobiología y ParasitologíaEuropean Union (UE)Ministerio de Educación y Ciencia (España)2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/165221https://doi.org/10.1099/ijs.0.64818-0reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Systematic and Evolutionary Microbiology, 57 (5), 900-905.QLK3-CT-2002-01972BMC2003-01344https://doi.org/10.1099/ijs.0.64818-0info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1652212026-06-17T12:51:07Z
dc.title.none.fl_str_mv Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
title Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
spellingShingle Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
Soto Ramírez, Nelís
title_short Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
title_full Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
title_fullStr Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
title_full_unstemmed Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
title_sort Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico
dc.creator.none.fl_str_mv Soto Ramírez, Nelís
Sánchez-Porro Álvarez, Cristina
Rosas, Soniris
González, Wildaomaris
Quiñones, Marian
Ventosa Ucero, Antonio
Montalvo Rodríguez, Rafael
author Soto Ramírez, Nelís
author_facet Soto Ramírez, Nelís
Sánchez-Porro Álvarez, Cristina
Rosas, Soniris
González, Wildaomaris
Quiñones, Marian
Ventosa Ucero, Antonio
Montalvo Rodríguez, Rafael
author_role author
author2 Sánchez-Porro Álvarez, Cristina
Rosas, Soniris
González, Wildaomaris
Quiñones, Marian
Ventosa Ucero, Antonio
Montalvo Rodríguez, Rafael
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
European Union (UE)
Ministerio de Educación y Ciencia (España)
description A Gram-negative, short rod to oval-shaped bacterium (strain MW2aT) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2aT was moderately halophilic, growing at NaCl concentrations in the range 0-25% (w/v) with optimum growth at 5% (w/v) NaCl. Growth occurred at 12-40°C (optimum, 30-35°C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Strain MW2aT was strictly aerobic. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Halomonas. The closest relative was Halomonas marisflavi, with 98.6% 16S rRNA gene sequence similarity. The DNA G + C content of strain MW2aT was 61.5 mol%, which is in the range of values for Halomonas species. DNA-DNA hybridization with H. marisflavi showed a relatedness of 42% and lower values were obtained with respect to other related Halomonas species. The major fatty acids were C16:0, C19:0 cyclo ω8c, C18:1 ω7c and C12:0 3-OH. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain Nw2aT represents a novel species within the genus Halomonas. The name Halomonas avicenniae sp. nov. is proposed, with strain MW2aT (= CECT 7193T =CCM 7396T) as the type strain.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/165221
https://doi.org/10.1099/ijs.0.64818-0
url https://hdl.handle.net/11441/165221
https://doi.org/10.1099/ijs.0.64818-0
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Systematic and Evolutionary Microbiology, 57 (5), 900-905.
QLK3-CT-2002-01972
BMC2003-01344
https://doi.org/10.1099/ijs.0.64818-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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