A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum
We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneu...
| Autores: | , , , , , , |
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| Tipo de documento: | artigo |
| Data de publicação: | 2001 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositório: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglês |
| OAI Identifier: | oai:dadun.unav.edu:10171/20099 |
| Acesso em linha: | https://hdl.handle.net/10171/20099 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Dextran amine Fluoro-Gold Interneuron |
| Resumo: | We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneurons. To this end, we have combined anterograde tracing using biotinylated dextran amine and retrograde neuroanatomical tracing with Fluoro-Gold. This dual tracing protocol was further implemented with the visualization of different subpopulations of striatal interneurons. The subsequent use of three different peroxidase substrates enabled us to unequivocally detect structures that were labeled within a three-color paradigm. |
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