Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments

Sediments underlying the solar salterns of S'Avall are anoxic hypersaline ecosystems dominated by anaerobic prokaryotes, and with the especial relevance of putative methanogenic archaea. Slurries from salt-saturated sediments, diluted in a gradient of salinity and incubated for > 4 years rev...

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Detalhes bibliográficos
Autores: Font-Verdera, Francisca, Liébana, Raquel, Rosselló-Mora, Ramón, Viver, Tomeu
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/353426
Acesso em linha:http://hdl.handle.net/10261/353426
https://api.elsevier.com/content/abstract/scopus_id/85177978020
Access Level:acceso abierto
Palavra-chave:Salinity
16S rRNA gene
Ampicillin
Anaerobic hypersaline sediments
Methane
Microcosms
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spelling Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sedimentsFont-Verdera, FranciscaLiébana, RaquelRosselló-Mora, RamónViver, TomeuSalinity16S rRNA geneAmpicillinAnaerobic hypersaline sedimentsMethaneMicrocosmsSediments underlying the solar salterns of S'Avall are anoxic hypersaline ecosystems dominated by anaerobic prokaryotes, and with the especial relevance of putative methanogenic archaea. Slurries from salt-saturated sediments, diluted in a gradient of salinity and incubated for > 4 years revealed that salt concentration was the major selection force that deterministically structured microbial communities. The dominant archaea in the original communities showed a decrease in alpha diversity with dilution accompanied by the increase of bacterial alpha diversity, being highest at 5% salts. Correspondingly, methanogens decreased and in turn sulfate reducers increased with decreasing salt concentrations. Methanogens especially dominated at 25%. Different concentrations of litter of Posidonia oceanica seagrass added as a carbon substrate, did not promote any clear relevant effect. However, the addition of ampicillin as selection pressure exerted important effects on the assemblage probably due to the removal of competitors or enhancers. The amended antibiotic enhanced methanogenesis in the concentrations ≤ 15% of salts, whereas it was depleted at salinities ≥ 20% revealing key roles of ampicillin-sensitive bacteria.This work was supported by the Spanish Ministry of Science, Innovation and Universities projects PGC2018-096956-B-C41, RTC-2017-6405-1, and PID2021-126114NB-C42, which were also supported with European Regional Development Fund (FEDER) funds. R.R.M. acknowledges the researcher mobility (grant numbers PRX18/00048 and PRX21/00043), F.F.V.’s the fellowship (grant number BES-2016-078138), and T,V, the “Margarita Salas” postdoctoral grant, all funded by the Spanish Ministry of Science, Innovation and Universities, and also within the framework of Recovery, Transformation and Resilience Plan, and funded by the European Union (NextGenerationEU), with the participation of the University of Balearic Islands (UIB). This research was carried out within the framework of the activities of the Spanish Government through the “Maria de Maeztu Centre of Excellence” accreditation to IMEDEA (CSIC-UIB) (CEX2021-001198).With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001198).Peer reviewedOxford University PressMinisterio de Ciencia, Innovación y Universidades (España)European CommissionUniversidad de Las Islas BalearesAgencia Estatal de Investigación (España)Font-Verdera, Francisca [0000-0001-7325-9329]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/353426https://api.elsevier.com/content/abstract/scopus_id/85177978020reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001198info:eu-repo/grantAgreement/AEI/BES-2016-078138/info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096956-B-C41info:eu-repo/grantAgreement/AEI//RTC-2017-6405-1info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126114NB-C42https://doi.org/10.1093/femsec/fiad146Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3534262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
title Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
spellingShingle Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
Font-Verdera, Francisca
Salinity
16S rRNA gene
Ampicillin
Anaerobic hypersaline sediments
Methane
Microcosms
title_short Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
title_full Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
title_fullStr Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
title_full_unstemmed Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
title_sort Impact of dilution on stochastically driven methanogenic microbial communities of hypersaline anoxic sediments
dc.creator.none.fl_str_mv Font-Verdera, Francisca
Liébana, Raquel
Rosselló-Mora, Ramón
Viver, Tomeu
author Font-Verdera, Francisca
author_facet Font-Verdera, Francisca
Liébana, Raquel
Rosselló-Mora, Ramón
Viver, Tomeu
author_role author
author2 Liébana, Raquel
Rosselló-Mora, Ramón
Viver, Tomeu
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Universidad de Las Islas Baleares
Agencia Estatal de Investigación (España)
Font-Verdera, Francisca [0000-0001-7325-9329]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Salinity
16S rRNA gene
Ampicillin
Anaerobic hypersaline sediments
Methane
Microcosms
topic Salinity
16S rRNA gene
Ampicillin
Anaerobic hypersaline sediments
Methane
Microcosms
description Sediments underlying the solar salterns of S'Avall are anoxic hypersaline ecosystems dominated by anaerobic prokaryotes, and with the especial relevance of putative methanogenic archaea. Slurries from salt-saturated sediments, diluted in a gradient of salinity and incubated for > 4 years revealed that salt concentration was the major selection force that deterministically structured microbial communities. The dominant archaea in the original communities showed a decrease in alpha diversity with dilution accompanied by the increase of bacterial alpha diversity, being highest at 5% salts. Correspondingly, methanogens decreased and in turn sulfate reducers increased with decreasing salt concentrations. Methanogens especially dominated at 25%. Different concentrations of litter of Posidonia oceanica seagrass added as a carbon substrate, did not promote any clear relevant effect. However, the addition of ampicillin as selection pressure exerted important effects on the assemblage probably due to the removal of competitors or enhancers. The amended antibiotic enhanced methanogenesis in the concentrations ≤ 15% of salts, whereas it was depleted at salinities ≥ 20% revealing key roles of ampicillin-sensitive bacteria.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/353426
https://api.elsevier.com/content/abstract/scopus_id/85177978020
url http://hdl.handle.net/10261/353426
https://api.elsevier.com/content/abstract/scopus_id/85177978020
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001198
info:eu-repo/grantAgreement/AEI/BES-2016-078138/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096956-B-C41
info:eu-repo/grantAgreement/AEI//RTC-2017-6405-1
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126114NB-C42
https://doi.org/10.1093/femsec/fiad146
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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