Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies

Osteosarcoma (OSA) is the most common bone tumor affecting the dog. The veterinary options for therapeutic management of OSA are limited and prognosis for such patients is poor. Oncolytic adenoviruses are attractive tools for experimental therapeutics as they can replicate and spread within tumors t...

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Autores: Alcayaga Miranda, Francisca Andrea, Cascalló, Manel, Rojas Expósito, Juan José|||0000-0002-3705-6767, Pastor Milán, Josep|||0000-0003-1702-9531, Alemany Bonastre, Ramon|||0000-0001-8660-550X
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:233822
Acceso en línea:https://ddd.uab.cat/record/233822
https://dx.doi.org/urn:doi:10.1038/cgt.2010.36
Access Level:acceso abierto
Palabra clave:Càncer
Teràpia genètica
Gossos
Malalties
Oncologia veterinària
Osteosarcoma
Dog
Carrier cells
Canine adenovirus
Oncolytic virus
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spelling Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodiesAlcayaga Miranda, Francisca AndreaCascalló, ManelRojas Expósito, Juan José|||0000-0002-3705-6767Pastor Milán, Josep|||0000-0003-1702-9531Alemany Bonastre, Ramon|||0000-0001-8660-550XCàncerTeràpia genèticaGossosMalaltiesOncologia veterinàriaOsteosarcomaOsteosarcomaDogCarrier cellsCanine adenovirusOncolytic virusOsteosarcoma (OSA) is the most common bone tumor affecting the dog. The veterinary options for therapeutic management of OSA are limited and prognosis for such patients is poor. Oncolytic adenoviruses are attractive tools for experimental therapeutics as they can replicate and spread within tumors to directly induce tumor destruction. However, a major impediment to systemic oncolytic adenoviruses injection is the presence of pre-existing neutralizing antibodies (Nabs). In this study, we investigated the effect of a replication-selective canine adenovirus (OCCAV) to treat OSA in the presence of Nabs and the use of canine OSA cells as carrier vehicles for evading Nabs. Our systemic biodistribution data indicated that canine tumor cells could successfully reach the tumor site and deliver OCCAV to tumor cells in an immunized mice model. Furthermore, the use of carrier cells also reduced adenovirus uptake by the liver. Importantly, OCCAV alone was not effective to control tumor growth in a pre-immunized xenograft mouse model. On the contrary, systemic antitumoral activity of carrier-cell OCCAV was evident even in the presence of circulating antibodies, which is a relevant result from a clinical point of view. These findings are of direct translational relevance for the future design of canine clinical trials. 22010-01-0120102010-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/233822https://dx.doi.org/urn:doi:10.1038/cgt.2010.36reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2338222026-06-06T12:50:31Z
dc.title.none.fl_str_mv Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
title Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
spellingShingle Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
Alcayaga Miranda, Francisca Andrea
Càncer
Teràpia genètica
Gossos
Malalties
Oncologia veterinària
Osteosarcoma
Osteosarcoma
Dog
Carrier cells
Canine adenovirus
Oncolytic virus
title_short Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
title_full Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
title_fullStr Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
title_full_unstemmed Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
title_sort Osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies
dc.creator.none.fl_str_mv Alcayaga Miranda, Francisca Andrea
Cascalló, Manel
Rojas Expósito, Juan José|||0000-0002-3705-6767
Pastor Milán, Josep|||0000-0003-1702-9531
Alemany Bonastre, Ramon|||0000-0001-8660-550X
author Alcayaga Miranda, Francisca Andrea
author_facet Alcayaga Miranda, Francisca Andrea
Cascalló, Manel
Rojas Expósito, Juan José|||0000-0002-3705-6767
Pastor Milán, Josep|||0000-0003-1702-9531
Alemany Bonastre, Ramon|||0000-0001-8660-550X
author_role author
author2 Cascalló, Manel
Rojas Expósito, Juan José|||0000-0002-3705-6767
Pastor Milán, Josep|||0000-0003-1702-9531
Alemany Bonastre, Ramon|||0000-0001-8660-550X
author2_role author
author
author
author
dc.subject.none.fl_str_mv Càncer
Teràpia genètica
Gossos
Malalties
Oncologia veterinària
Osteosarcoma
Osteosarcoma
Dog
Carrier cells
Canine adenovirus
Oncolytic virus
topic Càncer
Teràpia genètica
Gossos
Malalties
Oncologia veterinària
Osteosarcoma
Osteosarcoma
Dog
Carrier cells
Canine adenovirus
Oncolytic virus
description Osteosarcoma (OSA) is the most common bone tumor affecting the dog. The veterinary options for therapeutic management of OSA are limited and prognosis for such patients is poor. Oncolytic adenoviruses are attractive tools for experimental therapeutics as they can replicate and spread within tumors to directly induce tumor destruction. However, a major impediment to systemic oncolytic adenoviruses injection is the presence of pre-existing neutralizing antibodies (Nabs). In this study, we investigated the effect of a replication-selective canine adenovirus (OCCAV) to treat OSA in the presence of Nabs and the use of canine OSA cells as carrier vehicles for evading Nabs. Our systemic biodistribution data indicated that canine tumor cells could successfully reach the tumor site and deliver OCCAV to tumor cells in an immunized mice model. Furthermore, the use of carrier cells also reduced adenovirus uptake by the liver. Importantly, OCCAV alone was not effective to control tumor growth in a pre-immunized xenograft mouse model. On the contrary, systemic antitumoral activity of carrier-cell OCCAV was evident even in the presence of circulating antibodies, which is a relevant result from a clinical point of view. These findings are of direct translational relevance for the future design of canine clinical trials.
publishDate 2010
dc.date.none.fl_str_mv 2
2010-01-01
2010
2010-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/233822
https://dx.doi.org/urn:doi:10.1038/cgt.2010.36
url https://ddd.uab.cat/record/233822
https://dx.doi.org/urn:doi:10.1038/cgt.2010.36
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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