Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection

17 pages, 6 figures, 2 tables.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)

Detalhes bibliográficos
Autores: Saco, Amaro, Panebianco, Antonella, Blanco, Sofía, Novoa, Beatriz, Diz, Ángel P., Figueras Huerta, Antonio
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::a2da88ce77d8bd47d7c7e3ae731ec51e
Acesso em linha:http://hdl.handle.net/10261/258646
Access Level:Acceso aberto
Palavra-chave:Proteomic
Transcriptomic
Immune
Mussel
Bacterial infection
Mollusc
id ES_0ef49bf28b6e1fa09f7d646002e5dfa5
oai_identifier_str oai:dnet:digitalcsic_::a2da88ce77d8bd47d7c7e3ae731ec51e
network_acronym_str ES
network_name_str España
repository_id_str
spelling Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne InfectionSaco, AmaroPanebianco, AntonellaBlanco, SofíaNovoa, BeatrizDiz, Ángel P.Figueras Huerta, AntonioProteomicTranscriptomicImmuneMusselBacterial infectionMollusc17 pages, 6 figures, 2 tables.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)In recent years, the immune response of mussels (Mytilus galloprovincialis) has been studied at the transcriptomic level against several bacterial infections. As a result, different immune mechanisms have been revealed, including both conserved essential innate pathways and particularities of the mussel immune response according to its nature and environment. However, there is often a lack of functional verification because mussels are a non-model species and because transcriptomic and proteomic information is not always well correlated. In the current study, a high-throughput quantitative proteomics study coupled to LC-MS/MS analysis using isobaric tandem mass tags (TMTs) for protein labeling was employed to study the mussel gill immune response to a Vibrio splendidus bath (waterborne) infection at a functional protein level. A total of 4,242 proteins were identified and quantified, of which 226 were differentially expressed (DEPs) after infection, giving to the study a depth that was lacking in previous proteomic studies of the bivalve immune response. Modulated proteins evidenced an important cytoskeletal disruption caused by bacterial infection. A conserved network of associated proteins was modulated, regulating oxidative stress and NF-kB inflammatory responses and leading to innate immunity effectors. Proteomic results were submitted to an integrated analysis with those obtained in a previous transcriptomic approach with the same infection. Half of all the quantified proteins had a concordant transcriptomic expression trend, but this concordance increased when focusing on the DEPs. The correlation was higher within the immune-related DEPs, and the activation of the conserved NF-kB pro-inflammatory pathway was the main response in both approaches. The results of both techniques could be integrated to obtain a more complete vision of the responseThis research was funded by the Spanish AEI/EU-FEDER (RTI2018-095997-B-I00); the European Regional Development Fund (ERDF) Interreg V Spain—Portugal (0474_BLUEBIOLAB); the Consellería de Economía, Emprego e Industria—GAIN, Xunta de Galicia, project IN607B 2019/01 and EU H2020; project VIVALDI (678589); Xunta de Galicia (ED431C 2020/05) and FONDOS FEDER. AS was supported by a Spanish AEI/EU-FSE predoctoral contract PRE2019-090760Peer reviewedFrontiers MediaEuropean CommissionAgencia Estatal de Investigación (España)Xunta de GaliciaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/258646reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/678589info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095997-B-I00Saco, Amaro; Diz, Ángel P.; 2021; Mytilus galloprovincialis gills exposed to Vibrio splendidus waterborne infection [Dataset]; PRIDE; https://dx.doi.org/10.6019/PXD028043https://doi.org/10.3389/fmars.2021.735309Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::a2da88ce77d8bd47d7c7e3ae731ec51e2026-05-22T06:33:51Z
dc.title.none.fl_str_mv Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
title Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
spellingShingle Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
Saco, Amaro
Proteomic
Transcriptomic
Immune
Mussel
Bacterial infection
Mollusc
title_short Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
title_full Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
title_fullStr Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
title_full_unstemmed Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
title_sort Integration of Transcriptomics and Proteomics Improves the Characterization of the Role of Mussel Gills in a Bacterial Waterborne Infection
dc.creator.none.fl_str_mv Saco, Amaro
Panebianco, Antonella
Blanco, Sofía
Novoa, Beatriz
Diz, Ángel P.
Figueras Huerta, Antonio
author Saco, Amaro
author_facet Saco, Amaro
Panebianco, Antonella
Blanco, Sofía
Novoa, Beatriz
Diz, Ángel P.
Figueras Huerta, Antonio
author_role author
author2 Panebianco, Antonella
Blanco, Sofía
Novoa, Beatriz
Diz, Ángel P.
Figueras Huerta, Antonio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Xunta de Galicia
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Proteomic
Transcriptomic
Immune
Mussel
Bacterial infection
Mollusc
topic Proteomic
Transcriptomic
Immune
Mussel
Bacterial infection
Mollusc
description 17 pages, 6 figures, 2 tables.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/258646
url http://hdl.handle.net/10261/258646
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/678589
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095997-B-I00
Saco, Amaro; Diz, Ángel P.; 2021; Mytilus galloprovincialis gills exposed to Vibrio splendidus waterborne infection [Dataset]; PRIDE; https://dx.doi.org/10.6019/PXD028043
https://doi.org/10.3389/fmars.2021.735309
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403426764357632
score 15,228081

Registros relacionados