The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation

[EN]Cytokinesis is a crucial event in the cell cycle of all living cells. In fungal cells, it requires coordinated contraction of an actomyosin ring and synthesis of both plasmatic membrane and a septum structure that will constitute the new cell wall end. Schizosaccharomyces pombe contains four ess...

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Detalhes bibliográficos
Autores: Cortés, Juan Carlos G., Konomi, Mami, Martins, Ivone M., Muñoz, Javier, Moreno, María Belén, Osumi, Masako, Durán, Angel, Ribas, Juan Carlos
Tipo de documento: artigo
Data de publicação:2007
País:España
Recursos:Universidad de Salamanca (USAL)
Repositório:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/125256
Acesso em linha:http://hdl.handle.net/10366/125256
Access Level:Acceso aberto
Palavra-chave:Glucan synthase
Cell wall
Morphogenesis
Septation
Fission Yeast
Morfogénesis
Levaduras
Tabicación celular
2415 Biología molecular
2414.10 Micología (levaduras)
2407 Biología celular
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oai_identifier_str oai:gredos.usal.es:10366/125256
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repository_id_str
spelling The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formationBgs1p is responsible for primary septum formationCortés, Juan Carlos G.Konomi, MamiMartins, Ivone M.Muñoz, JavierMoreno, María BelénOsumi, MasakoDurán, AngelRibas, Juan CarlosGlucan synthaseCell wallMorphogenesisSeptationFission YeastMorfogénesisLevadurasTabicación celular2415 Biología molecular2414.10 Micología (levaduras)2407 Biología celular[EN]Cytokinesis is a crucial event in the cell cycle of all living cells. In fungal cells, it requires coordinated contraction of an actomyosin ring and synthesis of both plasmatic membrane and a septum structure that will constitute the new cell wall end. Schizosaccharomyces pombe contains four essential putative (1,3)β-D-glucan synthase catalytic subunits, Bgs1p to Bgs4p. Here we examined the function of Bgs1p in septation by studying the lethal phenotypes of bgs1 + shut-off and bgs1 Δ cells and demonstrated that Bgs1p is responsible and essential for linear (1,3)β-D-glucan and primary septum formation. bgs1 + shut-off generates a more than 300-fold Bgs1p reduction, but the septa still present large amounts of disorganized linear (1,3)β-D-glucan and partial primary septa. Conversely, both structures are absent in bgs1 Δ cells, where there is no Bgs1p. The septum analysis of bgs1+-repressed cells indicates that linear (1,3)β-D-glucan is necessary but not sufficient for primary septum formation. Linear (1,3)β-D-glucan is the polysaccharide that specifically interacts with the fluorochrome Calcofluor white in fission yeast. We also show that in the absence of Bgs1p abnormal septa are formed, but the cells cannot separate and eventually die.201520152007info:eu-repo/semantics/articlehttp://hdl.handle.net/10366/125256reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivs 3.0 Unportedhttps://creativecommons.org/licenses/by-nc-nd/3.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1252562026-06-07T06:28:51Z
dc.title.none.fl_str_mv The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
Bgs1p is responsible for primary septum formation
title The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
spellingShingle The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
Cortés, Juan Carlos G.
Glucan synthase
Cell wall
Morphogenesis
Septation
Fission Yeast
Morfogénesis
Levaduras
Tabicación celular
2415 Biología molecular
2414.10 Micología (levaduras)
2407 Biología celular
title_short The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
title_full The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
title_fullStr The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
title_full_unstemmed The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
title_sort The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
dc.creator.none.fl_str_mv Cortés, Juan Carlos G.
Konomi, Mami
Martins, Ivone M.
Muñoz, Javier
Moreno, María Belén
Osumi, Masako
Durán, Angel
Ribas, Juan Carlos
author Cortés, Juan Carlos G.
author_facet Cortés, Juan Carlos G.
Konomi, Mami
Martins, Ivone M.
Muñoz, Javier
Moreno, María Belén
Osumi, Masako
Durán, Angel
Ribas, Juan Carlos
author_role author
author2 Konomi, Mami
Martins, Ivone M.
Muñoz, Javier
Moreno, María Belén
Osumi, Masako
Durán, Angel
Ribas, Juan Carlos
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Glucan synthase
Cell wall
Morphogenesis
Septation
Fission Yeast
Morfogénesis
Levaduras
Tabicación celular
2415 Biología molecular
2414.10 Micología (levaduras)
2407 Biología celular
topic Glucan synthase
Cell wall
Morphogenesis
Septation
Fission Yeast
Morfogénesis
Levaduras
Tabicación celular
2415 Biología molecular
2414.10 Micología (levaduras)
2407 Biología celular
description [EN]Cytokinesis is a crucial event in the cell cycle of all living cells. In fungal cells, it requires coordinated contraction of an actomyosin ring and synthesis of both plasmatic membrane and a septum structure that will constitute the new cell wall end. Schizosaccharomyces pombe contains four essential putative (1,3)β-D-glucan synthase catalytic subunits, Bgs1p to Bgs4p. Here we examined the function of Bgs1p in septation by studying the lethal phenotypes of bgs1 + shut-off and bgs1 Δ cells and demonstrated that Bgs1p is responsible and essential for linear (1,3)β-D-glucan and primary septum formation. bgs1 + shut-off generates a more than 300-fold Bgs1p reduction, but the septa still present large amounts of disorganized linear (1,3)β-D-glucan and partial primary septa. Conversely, both structures are absent in bgs1 Δ cells, where there is no Bgs1p. The septum analysis of bgs1+-repressed cells indicates that linear (1,3)β-D-glucan is necessary but not sufficient for primary septum formation. Linear (1,3)β-D-glucan is the polysaccharide that specifically interacts with the fluorochrome Calcofluor white in fission yeast. We also show that in the absence of Bgs1p abnormal septa are formed, but the cells cannot separate and eventually die.
publishDate 2007
dc.date.none.fl_str_mv 2007
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/125256
url http://hdl.handle.net/10366/125256
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Unported
https://creativecommons.org/licenses/by-nc-nd/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Unported
https://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403397595070464
score 15,301629

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