Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests

19 Pág.

Detalhes bibliográficos
Autores: Moreno-Pérez, Alba, Pintado, Adrián, Murillo, Jesús, Caballo-Ponce, Eloy, Tegli, Stefania, Moretti, Chiaraluce, Rodríguez-Palenzuela, Pablo, Ramos, Cayo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/339492
Acesso em linha:http://hdl.handle.net/10261/339492
https://api.elsevier.com/content/abstract/scopus_id/85088441412
Access Level:acceso abierto
Palavra-chave:Pseudomonas savastanoi pathovars
Pseudomonas syringae
Comparative genomics
Host range
Phytotoxins
Type III effectors
Virulence factors
Woody hosts
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dc.title.none.fl_str_mv Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
title Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
spellingShingle Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
Moreno-Pérez, Alba
Pseudomonas savastanoi pathovars
Pseudomonas syringae
Comparative genomics
Host range
Phytotoxins
Type III effectors
Virulence factors
Woody hosts
title_short Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
title_full Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
title_fullStr Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
title_full_unstemmed Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
title_sort Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests
dc.creator.none.fl_str_mv Moreno-Pérez, Alba
Pintado, Adrián
Murillo, Jesús
Caballo-Ponce, Eloy
Tegli, Stefania
Moretti, Chiaraluce
Rodríguez-Palenzuela, Pablo
Ramos, Cayo
author Moreno-Pérez, Alba
author_facet Moreno-Pérez, Alba
Pintado, Adrián
Murillo, Jesús
Caballo-Ponce, Eloy
Tegli, Stefania
Moretti, Chiaraluce
Rodríguez-Palenzuela, Pablo
Ramos, Cayo
author_role author
author2 Pintado, Adrián
Murillo, Jesús
Caballo-Ponce, Eloy
Tegli, Stefania
Moretti, Chiaraluce
Rodríguez-Palenzuela, Pablo
Ramos, Cayo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
DSA3 research funds
Moreno-Pérez, Alba [0000-0001-5089-8654]
Pintado, Adrián [0000-0001-6045-3018]
Murillo, Jesús [0000-0001-6585-2421]
Caballo-Ponce, Eloy [0000-0003-0501-3321]
Tegli, Stefania [0000-0003-1297-186X]
Moretti, Chiaraluce [0000-0003-1500-7320]
Rodríguez-Palenzuela, Pablo [0000-0002-4963-9177]
Ramos, Cayo [0000-0002-2362-5041]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pseudomonas savastanoi pathovars
Pseudomonas syringae
Comparative genomics
Host range
Phytotoxins
Type III effectors
Virulence factors
Woody hosts
topic Pseudomonas savastanoi pathovars
Pseudomonas syringae
Comparative genomics
Host range
Phytotoxins
Type III effectors
Virulence factors
Woody hosts
description 19 Pág.
publishDate 2020
dc.date.none.fl_str_mv 2020
2023
2023
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info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/339492
https://api.elsevier.com/content/abstract/scopus_id/85088441412
url http://hdl.handle.net/10261/339492
https://api.elsevier.com/content/abstract/scopus_id/85088441412
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MICIU//FPI
info:eu-repo/grantAgreement/MECD//FPU14%2F05551
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-R
Centro de Biotecnología y Genómica de Plantas (CBGP)
https://doi.org/10.3389/fpls.2020.00973
Sí
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dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity TestsMoreno-Pérez, AlbaPintado, AdriánMurillo, JesúsCaballo-Ponce, EloyTegli, StefaniaMoretti, ChiaraluceRodríguez-Palenzuela, PabloRamos, CayoPseudomonas savastanoi pathovarsPseudomonas syringaeComparative genomicsHost rangePhytotoxinsType III effectorsVirulence factorsWoody hosts19 Pág.The study of host range determinants within the Pseudomonas syringae complex is gaining renewed attention due to its widespread distribution in non-agricultural environments, evidence of large variability in intra-pathovar host range, and the emergence of new epidemic diseases. This requires the establishment of appropriate model pathosystems facilitating integration of phenotypic, genomic and evolutionary data. Pseudomonas savastanoi pv. savastanoi is a model pathogen of the olive tree, and here we report a closed genome of strain NCPPB 3335, plus draft genome sequences of three strains isolated from oleander (pv. nerii), ash (pv. fraxini) and broom plants (pv. retacarpa). We then conducted a comparative genomic analysis of these four new genomes plus 16 publicly available genomes, representing 20 strains of these four P. savastanoi pathovars of woody hosts. Despite overlapping host ranges, cross-pathogenicity tests using four plant hosts clearly separated these pathovars and lead to pathovar reassignment of two strains. Critically, these functional assays were pivotal to reconcile phylogeny with host range and to define pathovar-specific genes repertoires. We report a pan-genome of 7,953 ortholog gene families and a total of 45 type III secretion system effector genes, including 24 core genes, four genes exclusive of pv. retacarpa and several genes encoding pathovar-specific truncations. Noticeably, the four pathovars corresponded with well-defined genetic lineages, with core genome phylogeny and hierarchical clustering of effector genes closely correlating with pathogenic specialization. Knot-inducing pathovars encode genes absent in the canker-inducing pv. fraxini, such as those related to indole acetic acid, cytokinins, rhizobitoxine, and a bacteriophytochrome. Other pathovar-exclusive genes encode type I, type II, type IV, and type VI secretion system proteins, the phytotoxine phevamine A, a siderophore, c-di-GMP-related proteins, methyl chemotaxis proteins, and a broad collection of transcriptional regulators and transporters of eight different superfamilies. Our combination of pathogenicity analyses and genomics tools allowed us to correctly assign strains to pathovars and to propose a repertoire of host range-related genes in the P. syringae complex.AM-P, AP, CR, and JM were supported by grants FPI/BES-2015-074847, FPU14/05551, AGL2017-82492-C2-1-R and AGL2017-82492-C2-2-R, respectively, from Ministerio de Ciencia, Innovación y Universidades (Spain), cofinanced by the Fondo Europeo de Desarrollo Regional (FEDER); CM was supported by DSA3 research funds “Fondo di base” (Italy).Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)European CommissionDSA3 research fundsMoreno-Pérez, Alba [0000-0001-5089-8654]Pintado, Adrián [0000-0001-6045-3018]Murillo, Jesús [0000-0001-6585-2421]Caballo-Ponce, Eloy [0000-0003-0501-3321]Tegli, Stefania [0000-0003-1297-186X]Moretti, Chiaraluce [0000-0003-1500-7320]Rodríguez-Palenzuela, Pablo [0000-0002-4963-9177]Ramos, Cayo [0000-0002-2362-5041]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/339492https://api.elsevier.com/content/abstract/scopus_id/85088441412reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICIU//FPIinfo:eu-repo/grantAgreement/MECD//FPU14%2F05551info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-RCentro de Biotecnología y Genómica de Plantas (CBGP)https://doi.org/10.3389/fpls.2020.00973Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3394922026-05-22T06:33:51Z
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