Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms

14 páginas, 5 figuras, 3 tablas

Detalhes bibliográficos
Autores: Rodríguez-López, P., Puga, Carmen H., Orgaz, Belén, López Cabo, Marta
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/154793
Acesso em linha:http://hdl.handle.net/10261/154793
Access Level:acceso abierto
Palavra-chave:Benzalkonium chloride
Biofilms
Disinfection
Fluorescence microscopy
Listeria monocytogenes
Escherichia coli
Pronase
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spelling Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilmsRodríguez-López, P.Puga, Carmen H.Orgaz, BelénLópez Cabo, MartaBenzalkonium chlorideBiofilmsDisinfectionFluorescence microscopyListeria monocytogenesEscherichia coliPronase14 páginas, 5 figuras, 3 tablasThis work presents the assessment of the effectivity of a pronase (PRN)-benzalkonium chloride (BAC) sequential treatment in removing Listeria monocytogenes–Escherichia coli dual-species biofilms grown on stainless steel (SS) using fluorescence microscopy and plate count assays. The effects of PRN-BAC on the occupied area (OA) by undamaged cells in 168 h dual-species samples were determined using a first-order factorial design. Empirical equations significantly (r2 = 0.927) described a negative individual effect of BAC and a negative interactive effect of PRN-BAC achieving OA reductions up to 46%. After treatment, high numbers of remaining attached and released viable and cultivable E. coli cells were detected in PRN-BAC combinations when low BAC concentrations were used. Therefore, at appropriate BAC doses, in addition to biofilm removal, sequential application of PRN and BAC represents an appealing strategy for pathogen control on SS surfaces while hindering the dispersion of live cells into the environmentThis research was financially supported by the Spanish Ministerio de Economia y Competitividad [ENZYMONO, AGL2010-22212-C02-02]. P. Rodriguez-Lopez acknowledges financial support from the FPI programme [grant number BES-2011-050544]Peer reviewedTaylor & FrancisConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/154793reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1080/08927014.2017.1356290Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1547932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
title Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
spellingShingle Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
Rodríguez-López, P.
Benzalkonium chloride
Biofilms
Disinfection
Fluorescence microscopy
Listeria monocytogenes
Escherichia coli
Pronase
title_short Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
title_full Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
title_fullStr Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
title_full_unstemmed Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
title_sort Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes–Escherichia coli dual-species biofilms
dc.creator.none.fl_str_mv Rodríguez-López, P.
Puga, Carmen H.
Orgaz, Belén
López Cabo, Marta
author Rodríguez-López, P.
author_facet Rodríguez-López, P.
Puga, Carmen H.
Orgaz, Belén
López Cabo, Marta
author_role author
author2 Puga, Carmen H.
Orgaz, Belén
López Cabo, Marta
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Benzalkonium chloride
Biofilms
Disinfection
Fluorescence microscopy
Listeria monocytogenes
Escherichia coli
Pronase
topic Benzalkonium chloride
Biofilms
Disinfection
Fluorescence microscopy
Listeria monocytogenes
Escherichia coli
Pronase
description 14 páginas, 5 figuras, 3 tablas
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/154793
url http://hdl.handle.net/10261/154793
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1080/08927014.2017.1356290
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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