Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories

The reprogramming of human somatic cells to induced pluripotent cells (iPSCs) has become a milestone and a paradigm shift in the field of regenerative medicine and human disease modeling including drug testing and genome editing. However, the molecular processes occurring during reprogramming and af...

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Autores: Dučić, Tanja, Sánchez-Mata, Alicia, Castillo-Sánchez, Jesús, Algarra González, Manuel, González-Muñoz, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/45260
Acceso en línea:https://hdl.handle.net/2454/45260
Access Level:acceso abierto
Palabra clave:Induced pluripotent stem cells (iPSCs)
Oocyte
Reprogramming
FTIR
Synchrotron spectroscopy
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spelling Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectoriesDučić, TanjaSánchez-Mata, AliciaCastillo-Sánchez, JesúsAlgarra González, ManuelGonzález-Muñoz, ElenaInduced pluripotent stem cells (iPSCs)OocyteReprogrammingFTIRSynchrotron spectroscopyThe reprogramming of human somatic cells to induced pluripotent cells (iPSCs) has become a milestone and a paradigm shift in the field of regenerative medicine and human disease modeling including drug testing and genome editing. However, the molecular processes occurring during reprogramming and affecting the pluripotent state acquired remain largely unknown. Of interest, different pluripotent states have been described depending on the reprogramming factors used and the oocyte has emerged as a valuable source of information for candidate factors. The present study investigates the molecular changes occurring in somatic cells during reprogramming with either canonical (OSK) or oocyte-based (AOX15) combinations using synchrotron-radiation Fourier transform infrared (SR FTIR) spectroscopy. The data acquired by SR FTIR indicates different representation and conformation of biological relevant macromolecules (lipids, nucleic acids, carbohydrates and proteins) depending on the reprogramming combination used and at different stages during the reprogramming process. Association analysis based on cells spectra suggest that pluripotency acquisition trajectories converge at late intermediate stages while they diverge at early stages. Our results suggest that OSK and AOX15 reprogramming operates through differential mechanisms affecting nucleic acids reorganization and day 10 comes out as a candidate hinge point to further study the molecular pathways involved in the reprogramming process. This study indicates that SR FTIR approach contribute unpaired information to distinguish pluripotent states and to decipher pluripotency acquisition roadmaps and landmarks that will enable advanced biomedical applications of iPSCs.The authors thank ALBA Synchrotron facility for beamtime allocation and financial support from the Proposal No. 2021085254 and excellent working conditions. E.G-M acknowledge financial support from Ministerio de Ciencia e Innovación del Gobierno de Espana ˜ (grant number PID2021-124033OB-I00) and from Consejería Economía y Conocimiento Junta de Andalucía-FEDER (grant number UMA18-FEDERJA-107). Funding for open access charge was provided by Universidad de Málaga / CBUA.ElsevierInstitute for Advanced Materials and Mathematics - INAMAT22023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/45260reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/AEI//PID2021-124033OB-I00© 2023 The Author(s). This is an open access article under the CC BY-NC-ND license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/452602026-06-17T12:41:47Z
dc.title.none.fl_str_mv Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
title Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
spellingShingle Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
Dučić, Tanja
Induced pluripotent stem cells (iPSCs)
Oocyte
Reprogramming
FTIR
Synchrotron spectroscopy
title_short Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
title_full Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
title_fullStr Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
title_full_unstemmed Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
title_sort Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories
dc.creator.none.fl_str_mv Dučić, Tanja
Sánchez-Mata, Alicia
Castillo-Sánchez, Jesús
Algarra González, Manuel
González-Muñoz, Elena
author Dučić, Tanja
author_facet Dučić, Tanja
Sánchez-Mata, Alicia
Castillo-Sánchez, Jesús
Algarra González, Manuel
González-Muñoz, Elena
author_role author
author2 Sánchez-Mata, Alicia
Castillo-Sánchez, Jesús
Algarra González, Manuel
González-Muñoz, Elena
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Institute for Advanced Materials and Mathematics - INAMAT2
dc.subject.none.fl_str_mv Induced pluripotent stem cells (iPSCs)
Oocyte
Reprogramming
FTIR
Synchrotron spectroscopy
topic Induced pluripotent stem cells (iPSCs)
Oocyte
Reprogramming
FTIR
Synchrotron spectroscopy
description The reprogramming of human somatic cells to induced pluripotent cells (iPSCs) has become a milestone and a paradigm shift in the field of regenerative medicine and human disease modeling including drug testing and genome editing. However, the molecular processes occurring during reprogramming and affecting the pluripotent state acquired remain largely unknown. Of interest, different pluripotent states have been described depending on the reprogramming factors used and the oocyte has emerged as a valuable source of information for candidate factors. The present study investigates the molecular changes occurring in somatic cells during reprogramming with either canonical (OSK) or oocyte-based (AOX15) combinations using synchrotron-radiation Fourier transform infrared (SR FTIR) spectroscopy. The data acquired by SR FTIR indicates different representation and conformation of biological relevant macromolecules (lipids, nucleic acids, carbohydrates and proteins) depending on the reprogramming combination used and at different stages during the reprogramming process. Association analysis based on cells spectra suggest that pluripotency acquisition trajectories converge at late intermediate stages while they diverge at early stages. Our results suggest that OSK and AOX15 reprogramming operates through differential mechanisms affecting nucleic acids reorganization and day 10 comes out as a candidate hinge point to further study the molecular pathways involved in the reprogramming process. This study indicates that SR FTIR approach contribute unpaired information to distinguish pluripotent states and to decipher pluripotency acquisition roadmaps and landmarks that will enable advanced biomedical applications of iPSCs.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/45260
url https://hdl.handle.net/2454/45260
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI//PID2021-124033OB-I00
dc.rights.none.fl_str_mv © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license.
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license.
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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