Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats
Hapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 1993 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/29443 |
| Acceso en línea: | https://hdl.handle.net/10171/29443 |
| Access Level: | acceso abierto |
| Palabra clave: | Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis |
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Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goatsDiaz-Aparicio, E. (E.)|||/items/edf37a8a-11c5-46b7-936e-c91650f4a5d3Aragon, V. (V.)|||/items/0a51fb7f-6d35-4aea-baf9-b8b756dcb744Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5Alonso-Urmeneta, B. (Begoña)|||/items/d382ba1f-2066-4506-b698-46544089b8a9Font, M. (M.)|||/items/ba772c70-a020-4884-bab3-5e1cf0e16eebMoreno, E. (Edgardo)|||/items/11186877-6440-423f-a77a-c63ec45c54b0Perez-Ortiz, S. (S.)|||/items/66052817-1526-4db5-a441-9d1bdbe8d1b4Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efaDiaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861cMoriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7bRadial immunodiffusion testLinked immunosorbent-assayAgar-gel immunodifussionMonoclonal-antibodiesBovine brucellosisEnzyme-immunoassayNative haptenAbortusLipopolysaccharidesMelitensisHapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (NH) of lipopolysaccharide core sugars. The polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (Brucella abortus biotype 1 [A serotype] and Brucella melitensis biotype 3 [AM serotype]), sheep (B. melitensis biotypes 1 [M serotype] and 3), and goats (B. melitensis biotype 1). The reverse radial immunodiffusion test with the NH from B. melitensis 16 M (serotype M) showed the highest sensitivity (89.6 to 97.3%), regardless of the host species and the serotype of the infecting Brucella sp. Y. enterocolitica O:9 NH (A serotype) was useful for diagnosing disease in cattle infected with B. abortus biotype 1, but not in cattle infected with B. melitensis biotype 3, sheep, or goats. The different results obtained with the serotype M and A polysaccharides and the sera from animals infected with M, A, and AM serotypes of Brucella spp. showed that in naturally infected animals, a large proportion of the antibodies are directed to or react with a previously defined common epitope(s) (J. T. Douglas and D. A. Palmer, J. Clin. Microbiol. 26:1353-1356, 1988) different from the A or M epitopes. By using the radial immunodiffusion test with B. melitensis 16M NH, it was possible to differentiate infected from vaccinated cattle, sheep, and goats with a sensitivity and specificity similar to that of the complement fixation test.American Society for MicrobiologyDadun. Depósito Académico Digital Universidad de Navarra20132013-07-0119931993-01-0119931993-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/29443reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/294432026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| spellingShingle |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats Diaz-Aparicio, E. (E.)|||/items/edf37a8a-11c5-46b7-936e-c91650f4a5d3 Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis |
| title_short |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_full |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_fullStr |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_full_unstemmed |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_sort |
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| dc.creator.none.fl_str_mv |
Diaz-Aparicio, E. (E.)|||/items/edf37a8a-11c5-46b7-936e-c91650f4a5d3 Aragon, V. (V.)|||/items/0a51fb7f-6d35-4aea-baf9-b8b756dcb744 Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5 Alonso-Urmeneta, B. (Begoña)|||/items/d382ba1f-2066-4506-b698-46544089b8a9 Font, M. (M.)|||/items/ba772c70-a020-4884-bab3-5e1cf0e16eeb Moreno, E. (Edgardo)|||/items/11186877-6440-423f-a77a-c63ec45c54b0 Perez-Ortiz, S. (S.)|||/items/66052817-1526-4db5-a441-9d1bdbe8d1b4 Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b |
| author |
Diaz-Aparicio, E. (E.)|||/items/edf37a8a-11c5-46b7-936e-c91650f4a5d3 |
| author_facet |
Diaz-Aparicio, E. (E.)|||/items/edf37a8a-11c5-46b7-936e-c91650f4a5d3 Aragon, V. (V.)|||/items/0a51fb7f-6d35-4aea-baf9-b8b756dcb744 Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5 Alonso-Urmeneta, B. (Begoña)|||/items/d382ba1f-2066-4506-b698-46544089b8a9 Font, M. (M.)|||/items/ba772c70-a020-4884-bab3-5e1cf0e16eeb Moreno, E. (Edgardo)|||/items/11186877-6440-423f-a77a-c63ec45c54b0 Perez-Ortiz, S. (S.)|||/items/66052817-1526-4db5-a441-9d1bdbe8d1b4 Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b |
| author_role |
author |
| author2 |
Aragon, V. (V.)|||/items/0a51fb7f-6d35-4aea-baf9-b8b756dcb744 Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5 Alonso-Urmeneta, B. (Begoña)|||/items/d382ba1f-2066-4506-b698-46544089b8a9 Font, M. (M.)|||/items/ba772c70-a020-4884-bab3-5e1cf0e16eeb Moreno, E. (Edgardo)|||/items/11186877-6440-423f-a77a-c63ec45c54b0 Perez-Ortiz, S. (S.)|||/items/66052817-1526-4db5-a441-9d1bdbe8d1b4 Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis |
| topic |
Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis |
| description |
Hapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (NH) of lipopolysaccharide core sugars. The polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (Brucella abortus biotype 1 [A serotype] and Brucella melitensis biotype 3 [AM serotype]), sheep (B. melitensis biotypes 1 [M serotype] and 3), and goats (B. melitensis biotype 1). The reverse radial immunodiffusion test with the NH from B. melitensis 16 M (serotype M) showed the highest sensitivity (89.6 to 97.3%), regardless of the host species and the serotype of the infecting Brucella sp. Y. enterocolitica O:9 NH (A serotype) was useful for diagnosing disease in cattle infected with B. abortus biotype 1, but not in cattle infected with B. melitensis biotype 3, sheep, or goats. The different results obtained with the serotype M and A polysaccharides and the sera from animals infected with M, A, and AM serotypes of Brucella spp. showed that in naturally infected animals, a large proportion of the antibodies are directed to or react with a previously defined common epitope(s) (J. T. Douglas and D. A. Palmer, J. Clin. Microbiol. 26:1353-1356, 1988) different from the A or M epitopes. By using the radial immunodiffusion test with B. melitensis 16M NH, it was possible to differentiate infected from vaccinated cattle, sheep, and goats with a sensitivity and specificity similar to that of the complement fixation test. |
| publishDate |
1993 |
| dc.date.none.fl_str_mv |
1993 1993-01-01 1993 1993-01-01 2013 2013-07-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/29443 |
| url |
https://hdl.handle.net/10171/29443 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869402936695586816 |
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15,228081 |