Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.

In previous works, our research group has successfully proved the use of subcellular vaccines based on poly(ε-caprolactone) (PEC) microparticles containing an antigenic extract of Brucella ovis (HS) against experimental brucellosis in both mice and rams. However, the successful exploitation of pharm...

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Detalhes bibliográficos
Autores: Estevan, M. (M.)|||/items/3e498094-cebe-44e4-85cd-8d847b32f858, Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d, Martínez-Galán, F.M. (Fernando María)|||/items/4744d6b5-3eec-427f-8920-290afb2cf85c, Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Formato: artículo
Fecha de publicación:2008
País:España
Recursos:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/19189
Acesso em linha:https://hdl.handle.net/10171/19189
Access Level:acceso abierto
Palavra-chave:Adjuvant
Brucellosis
Microparticles
Stability
Vaccine
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spelling Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.Estevan, M. (M.)|||/items/3e498094-cebe-44e4-85cd-8d847b32f858Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1dMartínez-Galán, F.M. (Fernando María)|||/items/4744d6b5-3eec-427f-8920-290afb2cf85cIrache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173AdjuvantBrucellosisMicroparticlesStabilityVaccineIn previous works, our research group has successfully proved the use of subcellular vaccines based on poly(ε-caprolactone) (PEC) microparticles containing an antigenic extract of Brucella ovis (HS) against experimental brucellosis in both mice and rams. However, the successful exploitation of pharmaceutical products, and therefore of this product as veterinary vaccine, requires preservation of both biological activity and native structure in all steps of development from purification to storage. In this context, we have carried out an accelerated stability study to evaluate the relative stability of HS when loading in PEC microparticles. For this purpose, freeze-dried microparticles were stored at 40±1°C and 75% RH as a preliminary analysis of a stability testing. The results showed that both physicochemical (size, morphology, antigen content, release profile) and biological (integrity and antigenicity of the HS) properties were preserved after 6 months of storage. On the contrary, after 1 year of storage, the HS release profile was dramatically affected probably due to a progressive loss of the polymer microstructure. In addition, the degradation and loss of the antigenicity of the HS components was also evident by SDS-PAGE and immunoblotting analysis. In fact, after 12 months of storage, only the integrity and antigenicity of two of the major protective proteins of the HS antigenic complex were preserved.SpringerDadun. Depósito Académico Digital Universidad de Navarra20112011-10-2220082008-01-0120082008-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/19189reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/191892026-06-21T12:47:57Z
dc.title.none.fl_str_mv Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
title Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
spellingShingle Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
Estevan, M. (M.)|||/items/3e498094-cebe-44e4-85cd-8d847b32f858
Adjuvant
Brucellosis
Microparticles
Stability
Vaccine
title_short Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
title_full Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
title_fullStr Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
title_full_unstemmed Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
title_sort Stability of poly(epsilon-caprolactone) microparticles containing Brucella ovis antigens as a vaccine delivery system against brucellosis.
dc.creator.none.fl_str_mv Estevan, M. (M.)|||/items/3e498094-cebe-44e4-85cd-8d847b32f858
Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d
Martínez-Galán, F.M. (Fernando María)|||/items/4744d6b5-3eec-427f-8920-290afb2cf85c
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
author Estevan, M. (M.)|||/items/3e498094-cebe-44e4-85cd-8d847b32f858
author_facet Estevan, M. (M.)|||/items/3e498094-cebe-44e4-85cd-8d847b32f858
Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d
Martínez-Galán, F.M. (Fernando María)|||/items/4744d6b5-3eec-427f-8920-290afb2cf85c
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
author_role author
author2 Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d
Martínez-Galán, F.M. (Fernando María)|||/items/4744d6b5-3eec-427f-8920-290afb2cf85c
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
author2_role author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Adjuvant
Brucellosis
Microparticles
Stability
Vaccine
topic Adjuvant
Brucellosis
Microparticles
Stability
Vaccine
description In previous works, our research group has successfully proved the use of subcellular vaccines based on poly(ε-caprolactone) (PEC) microparticles containing an antigenic extract of Brucella ovis (HS) against experimental brucellosis in both mice and rams. However, the successful exploitation of pharmaceutical products, and therefore of this product as veterinary vaccine, requires preservation of both biological activity and native structure in all steps of development from purification to storage. In this context, we have carried out an accelerated stability study to evaluate the relative stability of HS when loading in PEC microparticles. For this purpose, freeze-dried microparticles were stored at 40±1°C and 75% RH as a preliminary analysis of a stability testing. The results showed that both physicochemical (size, morphology, antigen content, release profile) and biological (integrity and antigenicity of the HS) properties were preserved after 6 months of storage. On the contrary, after 1 year of storage, the HS release profile was dramatically affected probably due to a progressive loss of the polymer microstructure. In addition, the degradation and loss of the antigenicity of the HS components was also evident by SDS-PAGE and immunoblotting analysis. In fact, after 12 months of storage, only the integrity and antigenicity of two of the major protective proteins of the HS antigenic complex were preserved.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01
2008
2008-01-01
2011
2011-10-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/19189
url https://hdl.handle.net/10171/19189
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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