Expression of somatostatin receptors in human melanoma cell lines: effect of two different somatostatin analogues, octreotide and SOM230, on cell proliferation

Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines w...

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Detalhes bibliográficos
Autores: Martínez Alonso, Montserrat, Llecha Cano, Núria, Mayorga Mayorga, Maritza Elfride, Sorolla Bardají, Anabel, Dolcet Roca, Xavier, Sanmartín Novell, Verònica, Abal Diaz, Leandro, Casanova i Seuma, Josep M. (Josep Manel), Baradad Brusau, Manuel, Yeramian Hakim, Andree, Egido Garcia, Ramon Maria, Puig, Susana, Vilella, Ramón, Matias-Guiu, Xavier, Martí Laborda, Rosa Ma.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/41662
Acesso em linha:http://hdl.handle.net/10459.1/41662
Access Level:acceso abierto
Palavra-chave:Malignant melanoma
Somatostatin analogues
Octreotide
SOM230
Pell -- Càncer
Octreotida
Somatostatina
Medicaments antineoplàstics
Melanoma
Apoptosi
Descrição
Resumo:Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was less than 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated.

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