Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity

Canine leishmaniosis (CanL) is a growing health problem for which vaccination is a crucial tool for the control of disease. The successful development of an effective vaccine against this disease relies on eliciting a robust and enduring T-cell immune response involving the activation of CD4+ Th1 an...

ver descrição completa

Detalhes bibliográficos
Autores: Hurtado Morillas, Clara, Martínez Rodrigo, Abel, Orden Gutiérrez, José Antonio, De Urbina Fuentes, Laura, Mas Zubiri, Alicia, Domínguez Bernal, Gustavo Ramón
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103346
Acesso em linha:https://hdl.handle.net/20.500.14352/103346
Access Level:acceso abierto
Palavra-chave:636.09
Canine leishmaniosis
PLGA nanoparticles
Multi-epitope peptide
Nanovaccine
LetiFend®
Leishmania infantum
Veterinaria
3109 Ciencias Veterinarias
id ES_018a1d5853484bc7657c9d8113bd0f9d
oai_identifier_str oai:docta.ucm.es:20.500.14352/103346
network_acronym_str ES
network_name_str España
repository_id_str
spelling Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell ImmunityHurtado Morillas, ClaraMartínez Rodrigo, AbelOrden Gutiérrez, José AntonioDe Urbina Fuentes, LauraMas Zubiri, AliciaDomínguez Bernal, Gustavo Ramón636.09Canine leishmaniosisPLGA nanoparticlesMulti-epitope peptideNanovaccineLetiFend®Leishmania infantumVeterinaria3109 Ciencias VeterinariasCanine leishmaniosis (CanL) is a growing health problem for which vaccination is a crucial tool for the control of disease. The successful development of an effective vaccine against this disease relies on eliciting a robust and enduring T-cell immune response involving the activation of CD4+ Th1 and CD8+ T-cells. This study aimed to evaluate the immunogenicity and prophylactic efficacy of a novel nanovaccine comprising a multi-epitope peptide, known as HisDTC, encapsulated in PLGA nanoparticles against Leishmania infantum infection in the murine model. The encapsulation strategy was designed to enhance antigen loading and sustain release, ensuring prolonged exposure to the immune system. Our results showed that mice immunized with PLGA-encapsulated HisDTC exhibited a significant reduction in the parasite load in the liver and spleen over both short and long-term duration. This reduction was associated with a cellular immune profile marked by elevated levels of pro-inflammatory cytokines, such as IFN-γ, and the generation of memory T cells. In conclusion, the current study establishes that PLGA-encapsulated HisDTC can promote effective and long-lasting T-cell responses against L. infantum in the murine model. These findings underscore the potential utility of multi-epitope vaccines, in conjunction with appropriate delivery systems, as an alternative strategy for CanL control.MDPIUniversidad Complutense de Madrid20242024-02-1320242024-02-13journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103346reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMCI Not available PID2019-106487RB-I00 10.13039open accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1033462026-06-02T12:44:21Z
dc.title.none.fl_str_mv Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
spellingShingle Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
Hurtado Morillas, Clara
636.09
Canine leishmaniosis
PLGA nanoparticles
Multi-epitope peptide
Nanovaccine
LetiFend®
Leishmania infantum
Veterinaria
3109 Ciencias Veterinarias
title_short Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_full Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_fullStr Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_full_unstemmed Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_sort Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
dc.creator.none.fl_str_mv Hurtado Morillas, Clara
Martínez Rodrigo, Abel
Orden Gutiérrez, José Antonio
De Urbina Fuentes, Laura
Mas Zubiri, Alicia
Domínguez Bernal, Gustavo Ramón
author Hurtado Morillas, Clara
author_facet Hurtado Morillas, Clara
Martínez Rodrigo, Abel
Orden Gutiérrez, José Antonio
De Urbina Fuentes, Laura
Mas Zubiri, Alicia
Domínguez Bernal, Gustavo Ramón
author_role author
author2 Martínez Rodrigo, Abel
Orden Gutiérrez, José Antonio
De Urbina Fuentes, Laura
Mas Zubiri, Alicia
Domínguez Bernal, Gustavo Ramón
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Canine leishmaniosis
PLGA nanoparticles
Multi-epitope peptide
Nanovaccine
LetiFend®
Leishmania infantum
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Canine leishmaniosis
PLGA nanoparticles
Multi-epitope peptide
Nanovaccine
LetiFend®
Leishmania infantum
Veterinaria
3109 Ciencias Veterinarias
description Canine leishmaniosis (CanL) is a growing health problem for which vaccination is a crucial tool for the control of disease. The successful development of an effective vaccine against this disease relies on eliciting a robust and enduring T-cell immune response involving the activation of CD4+ Th1 and CD8+ T-cells. This study aimed to evaluate the immunogenicity and prophylactic efficacy of a novel nanovaccine comprising a multi-epitope peptide, known as HisDTC, encapsulated in PLGA nanoparticles against Leishmania infantum infection in the murine model. The encapsulation strategy was designed to enhance antigen loading and sustain release, ensuring prolonged exposure to the immune system. Our results showed that mice immunized with PLGA-encapsulated HisDTC exhibited a significant reduction in the parasite load in the liver and spleen over both short and long-term duration. This reduction was associated with a cellular immune profile marked by elevated levels of pro-inflammatory cytokines, such as IFN-γ, and the generation of memory T cells. In conclusion, the current study establishes that PLGA-encapsulated HisDTC can promote effective and long-lasting T-cell responses against L. infantum in the murine model. These findings underscore the potential utility of multi-epitope vaccines, in conjunction with appropriate delivery systems, as an alternative strategy for CanL control.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-02-13
2024
2024-02-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103346
url https://hdl.handle.net/20.500.14352/103346
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MCI Not available PID2019-106487RB-I00 10.13039
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402591160434688
score 15,223283

Registos relacionados