Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc

Supplementary Table 1: Primers used in this work.

Detalhes bibliográficos
Autores: Burciaga-Monge, Alma, López-Tubau, Joan Manel, Laibach, Natalie, Deng, Cuiyun, Ferrer, Albert, Altabella, Teresa
Tipo de documento: conjunto de datos
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/329665
Acesso em linha:http://hdl.handle.net/10261/329665
Access Level:Acceso aberto
Palavra-chave:CRISPR-Cas9
Germination
Lipid droplets
Phytosterols
Senescence
Sterol acyltransferase
Steryl ester
Tomato
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spelling Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.docBurciaga-Monge, AlmaLópez-Tubau, Joan ManelLaibach, NatalieDeng, CuiyunFerrer, AlbertAltabella, TeresaCRISPR-Cas9GerminationLipid dropletsPhytosterolsSenescenceSterol acyltransferaseSteryl esterTomatoSupplementary Table 1: Primers used in this work.Steryl esters (SE) are stored in cytoplasmic lipid droplets and serve as a reservoir of sterols that helps to maintain free sterols (FS) homeostasis in cell membranes throughout plant growth and development, and provides the FS needed to meet the high demand of these key plasma membrane components during rapid plant organ growth and expansion. SE are also involved in the recycling of sterols and fatty acids released from membranes during plant tissues senescence. SE are synthesized by sterol acyltransferases, which catalyze the transfer of long-chain fatty acid groups to the hydroxyl group at C3 position of FS. Depending on the donor substrate, these enzymes are called acyl-CoA:sterol acyltransferases (ASAT), when the substrate is a long-chain acyl-CoA, and phospholipid:sterol acyltransferases (PSAT), which use a phospholipid as a donor substrate. We have recently identified and preliminary characterized the tomato (Solanum lycopersicum cv. Micro-Tom) SlASAT1 and SlPSAT1 enzymes. To gain further insight into the biological role of these enzymes and SE biosynthesis in tomato, we generated and characterized CRISPR/Cas9 single knock-out mutants lacking SlPSAT1 (slpsat1) and SlASAT1 (slasat1), as well as the double mutant slpsat1 x slasat1. Analysis of FS and SE profiles in seeds and leaves of the single and double mutants revealed a strong depletion of SE in slpsat1, that was even more pronounced in the slpsat1 x slasat1 mutant, while an increase of SE levels was observed in slasat1. Moreover, SlPSAT1 and SlASAT1 inactivation affected in different ways several important cellular and physiological processes, like leaf lipid bo1dies formation, seed germination speed, leaf senescence, and the plant size. Altogether, our results indicate that SlPSAT1 has a predominant role in tomato SE biosynthesis while SlASAT1 would mainly regulate the flux of the sterol pathway. It is also worth to mention that some of the metabolic and physiological responses in the tomato mutants lacking functional SlPSAT1 or SlASAT1 are different from those previously reported in Arabidopsis, being remarkable the synergistic effect of SlASAT1 inactivation in the absence of a functional SlPSAT1 on the early germination and premature senescence phenotypes.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/mswordhttp://hdl.handle.net/10261/329665reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésBurciaga-Monge, Alma; López-Tubau, Joan Manel; Laibach, Natalie; Deng, Cuiyun; Ferrer, Albert; Altabella, Teresa. Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes. http://dx.doi.org/10.3389/fpls.2022.984100. http://hdl.handle.net/10261/289424https://doi.org/10.3389/fpls.2022.984100.s001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3296652026-05-22T06:33:51Z
dc.title.none.fl_str_mv Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
title Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
spellingShingle Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
Burciaga-Monge, Alma
CRISPR-Cas9
Germination
Lipid droplets
Phytosterols
Senescence
Sterol acyltransferase
Steryl ester
Tomato
title_short Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
title_full Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
title_fullStr Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
title_full_unstemmed Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
title_sort Table_1_Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.doc
dc.creator.none.fl_str_mv Burciaga-Monge, Alma
López-Tubau, Joan Manel
Laibach, Natalie
Deng, Cuiyun
Ferrer, Albert
Altabella, Teresa
author Burciaga-Monge, Alma
author_facet Burciaga-Monge, Alma
López-Tubau, Joan Manel
Laibach, Natalie
Deng, Cuiyun
Ferrer, Albert
Altabella, Teresa
author_role author
author2 López-Tubau, Joan Manel
Laibach, Natalie
Deng, Cuiyun
Ferrer, Albert
Altabella, Teresa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CRISPR-Cas9
Germination
Lipid droplets
Phytosterols
Senescence
Sterol acyltransferase
Steryl ester
Tomato
topic CRISPR-Cas9
Germination
Lipid droplets
Phytosterols
Senescence
Sterol acyltransferase
Steryl ester
Tomato
description Supplementary Table 1: Primers used in this work.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/329665
url http://hdl.handle.net/10261/329665
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Burciaga-Monge, Alma; López-Tubau, Joan Manel; Laibach, Natalie; Deng, Cuiyun; Ferrer, Albert; Altabella, Teresa. Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes. http://dx.doi.org/10.3389/fpls.2022.984100. http://hdl.handle.net/10261/289424
https://doi.org/10.3389/fpls.2022.984100.s001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/msword
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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