The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects

Transcriptome analyses using high-throughput methodologies allow a deeper understanding of biological functions in different cell types/tissues. The present study provides an RNA-seq profiling of human sperm mRNAs and lncRNAs (messenger and long non-coding RNAs) in a well-characterized population of...

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Autores: Corral-Vazquez, Celia|||0000-0002-0621-4590, Blanco, Joan|||0000-0003-0647-3856, Aiese Cigliano, Riccardo|||0000-0002-9058-6994, Sarrate Navas, Zaida|||0000-0001-9677-1376, Rivera-Egea, Rocio, Vidal, Francesca|||0000-0002-0985-7348, Garrido, Nicolás|||0000-0001-8271-5218, Daub, Carsten, Anton, Ester|||0000-0002-8914-2667
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:306504
Acesso em linha:https://ddd.uab.cat/record/306504
https://dx.doi.org/urn:doi:10.1093/molehr/gaab035
Access Level:Acceso aberto
Palavra-chave:Sperm
RNA
Lncrna
Fertility
RNA-seq
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spelling The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effectsCorral-Vazquez, Celia|||0000-0002-0621-4590Blanco, Joan|||0000-0003-0647-3856Aiese Cigliano, Riccardo|||0000-0002-9058-6994Sarrate Navas, Zaida|||0000-0001-9677-1376Rivera-Egea, RocioVidal, Francesca|||0000-0002-0985-7348Garrido, Nicolás|||0000-0001-8271-5218Daub, CarstenAnton, Ester|||0000-0002-8914-2667SpermRNALncrnaFertilityRNA-seqTranscriptome analyses using high-throughput methodologies allow a deeper understanding of biological functions in different cell types/tissues. The present study provides an RNA-seq profiling of human sperm mRNAs and lncRNAs (messenger and long non-coding RNAs) in a well-characterized population of fertile individuals. Sperm RNA was extracted from twelve ejaculate samples under strict quality controls. Poly(A)-transcripts were sequenced and aligned to the human genome. mRNAs and lncRNAs were classified according to their mean expression values (FPKM=Fragments Per Kilobase of transcript per Million mapped reads) and integrity. Gene Ontology analysis of the Expressed and Highly Expressed mRNAs showed an involvement in diverse reproduction processes, while the Ubiquitously Expressed and Highly Stable mRNAs were mainly involved in spermatogenesis. Transcription Factor enrichment analyses revealed that the Highly Expressed and Ubiquitously Expressed sperm mRNAs were primarily regulated by zinc-fingers and spermatogenesis-related proteins. Regarding the Expressed lncRNAs, only one-third of their potential targets corresponded to Expressed mRNAs and were enriched in cell-cycle regulation processes. The remaining two-thirds were absent in sperm and were enriched in embryogenesis-related processes. A significant amount of post-testicular sperm mRNAs and lncRNAs was also detected. Even though our study is solely directed to the poly-A fraction of sperm transcripts, results indicate that both sperm mRNAs and lncRNAs constitute a footprint of previous spermatogenesis events and are configured to affect the first stages of embryo development. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/306504https://dx.doi.org/urn:doi:10.1093/molehr/gaab035reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-00503open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3065042026-06-06T12:50:31Z
dc.title.none.fl_str_mv The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
title The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
spellingShingle The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
Corral-Vazquez, Celia|||0000-0002-0621-4590
Sperm
RNA
Lncrna
Fertility
RNA-seq
title_short The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
title_full The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
title_fullStr The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
title_full_unstemmed The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
title_sort The RNA content of human sperm reflects prior events in spermatogenesis and potential post-fertilization effects
dc.creator.none.fl_str_mv Corral-Vazquez, Celia|||0000-0002-0621-4590
Blanco, Joan|||0000-0003-0647-3856
Aiese Cigliano, Riccardo|||0000-0002-9058-6994
Sarrate Navas, Zaida|||0000-0001-9677-1376
Rivera-Egea, Rocio
Vidal, Francesca|||0000-0002-0985-7348
Garrido, Nicolás|||0000-0001-8271-5218
Daub, Carsten
Anton, Ester|||0000-0002-8914-2667
author Corral-Vazquez, Celia|||0000-0002-0621-4590
author_facet Corral-Vazquez, Celia|||0000-0002-0621-4590
Blanco, Joan|||0000-0003-0647-3856
Aiese Cigliano, Riccardo|||0000-0002-9058-6994
Sarrate Navas, Zaida|||0000-0001-9677-1376
Rivera-Egea, Rocio
Vidal, Francesca|||0000-0002-0985-7348
Garrido, Nicolás|||0000-0001-8271-5218
Daub, Carsten
Anton, Ester|||0000-0002-8914-2667
author_role author
author2 Blanco, Joan|||0000-0003-0647-3856
Aiese Cigliano, Riccardo|||0000-0002-9058-6994
Sarrate Navas, Zaida|||0000-0001-9677-1376
Rivera-Egea, Rocio
Vidal, Francesca|||0000-0002-0985-7348
Garrido, Nicolás|||0000-0001-8271-5218
Daub, Carsten
Anton, Ester|||0000-0002-8914-2667
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sperm
RNA
Lncrna
Fertility
RNA-seq
topic Sperm
RNA
Lncrna
Fertility
RNA-seq
description Transcriptome analyses using high-throughput methodologies allow a deeper understanding of biological functions in different cell types/tissues. The present study provides an RNA-seq profiling of human sperm mRNAs and lncRNAs (messenger and long non-coding RNAs) in a well-characterized population of fertile individuals. Sperm RNA was extracted from twelve ejaculate samples under strict quality controls. Poly(A)-transcripts were sequenced and aligned to the human genome. mRNAs and lncRNAs were classified according to their mean expression values (FPKM=Fragments Per Kilobase of transcript per Million mapped reads) and integrity. Gene Ontology analysis of the Expressed and Highly Expressed mRNAs showed an involvement in diverse reproduction processes, while the Ubiquitously Expressed and Highly Stable mRNAs were mainly involved in spermatogenesis. Transcription Factor enrichment analyses revealed that the Highly Expressed and Ubiquitously Expressed sperm mRNAs were primarily regulated by zinc-fingers and spermatogenesis-related proteins. Regarding the Expressed lncRNAs, only one-third of their potential targets corresponded to Expressed mRNAs and were enriched in cell-cycle regulation processes. The remaining two-thirds were absent in sperm and were enriched in embryogenesis-related processes. A significant amount of post-testicular sperm mRNAs and lncRNAs was also detected. Even though our study is solely directed to the poly-A fraction of sperm transcripts, results indicate that both sperm mRNAs and lncRNAs constitute a footprint of previous spermatogenesis events and are configured to affect the first stages of embryo development.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/306504
https://dx.doi.org/urn:doi:10.1093/molehr/gaab035
url https://ddd.uab.cat/record/306504
https://dx.doi.org/urn:doi:10.1093/molehr/gaab035
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-00503
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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