Parameters for the germination of elite fungal biocontrol strains

The marketing of microbial pesticides has increased exponentially in recent years. Almost 70% of the products marketed use as active ingredient conidia of biocontrol fungi, and One of the most frequently used criteria to evaluate the quality of these products is conidial germination test, Specifical...

ver descrição completa

Detalhes bibliográficos
Autores: Uribe Gutiérrez, Liz, Santos-Diaz , Adriana
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:Ecuador
Recursos:Universidad Central del Ecuador
Repositório:Revista Siembra
Idioma:espanhol
inglês
OAI Identifier:oai:revistadigital.uce.edu.ec:article/5631
Acesso em linha:https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631
Access Level:Acceso aberto
Palavra-chave:conidios
calidad
bioinsumos
viabilidad
conidia
quality
biopesticides
viability
id EC_a6ccc85dd04e5ecc66d36cb0c10a8d36
oai_identifier_str oai:revistadigital.uce.edu.ec:article/5631
network_acronym_str EC
network_name_str Ecuador
repository_id_str
dc.title.none.fl_str_mv Parameters for the germination of elite fungal biocontrol strains
Parámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladores
title Parameters for the germination of elite fungal biocontrol strains
spellingShingle Parameters for the germination of elite fungal biocontrol strains
Uribe Gutiérrez, Liz
conidios
calidad
bioinsumos
viabilidad
conidia
quality
biopesticides
viability
title_short Parameters for the germination of elite fungal biocontrol strains
title_full Parameters for the germination of elite fungal biocontrol strains
title_fullStr Parameters for the germination of elite fungal biocontrol strains
title_full_unstemmed Parameters for the germination of elite fungal biocontrol strains
title_sort Parameters for the germination of elite fungal biocontrol strains
dc.creator.none.fl_str_mv Uribe Gutiérrez, Liz
Santos-Diaz , Adriana
author Uribe Gutiérrez, Liz
author_facet Uribe Gutiérrez, Liz
Santos-Diaz , Adriana
author_role author
author2 Santos-Diaz , Adriana
author2_role author
dc.subject.none.fl_str_mv conidios
calidad
bioinsumos
viabilidad
conidia
quality
biopesticides
viability
topic conidios
calidad
bioinsumos
viabilidad
conidia
quality
biopesticides
viability
description The marketing of microbial pesticides has increased exponentially in recent years. Almost 70% of the products marketed use as active ingredient conidia of biocontrol fungi, and One of the most frequently used criteria to evaluate the quality of these products is conidial germination test, Specifically, this methodology is used to select and assess the viability and vigor of elite strains with biological potential. Some of the factors that influence conidial germination over time, include the selection of culture medium, temperature, incubation time, and the addition of germination synchronizing agents. The optimization for each elite strain allows the characterization of a microorganism behavior over time, ensuring its biological activity and effective management as a biological resource for commercial purposes. The main objective of this study was to identify the optimal conditions (culture media, incubation time, and synchronizing agent concentration) for the germination of three elite strains: Trichoderma koningiopsis (Th003), Lecanicillium lecanii (Vl026), and Metarhizium robertsii (Mt004), which are active ingredients in biopesticides. As a result, the optimal conditions selected are as follows: agar-water medium with 0.0003 % benomyl for Th003 with an incubation period of 18 hours, agar-water medium with 0.0003 % benomyl for Vl026 with an incubation period of 24 hours, and PDA agar supplemented with 0.0003 % benomyl for Mt004 with an incubation period of 18 hours. These conditions will allow a precise evaluation of the germination of the biological control agents ensuring over time their viability and vigor as active ingredients in biopesticides.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631
10.29166/siembra.v11i1.5631
url https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631
identifier_str_mv 10.29166/siembra.v11i1.5631
dc.language.none.fl_str_mv spa
eng
language spa
eng
dc.relation.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/7650
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/9882
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/8251
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/8252
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/7922
dc.rights.none.fl_str_mv Derechos de autor 2024 Liz Uribe Gutiérrez, Adriana Santos-Diaz
https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2024 Liz Uribe Gutiérrez, Adriana Santos-Diaz
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/html
application/zip
text/xml
dc.publisher.none.fl_str_mv Universidad Central del Ecuador
publisher.none.fl_str_mv Universidad Central del Ecuador
dc.source.none.fl_str_mv Siembra; Vol. 11 No. 1 (2024); e5631
Siembra; Vol. 11 Núm. 1 (2024); e5631
2477-8850
1390-8928
10.29166/siembra.v11i1
reponame:Revista Siembra
instname:Universidad Central del Ecuador
instacron:UCE
instname_str Universidad Central del Ecuador
instacron_str UCE
institution UCE
reponame_str Revista Siembra
collection Revista Siembra
repository.name.fl_str_mv Revista Siembra - Universidad Central del Ecuador
repository.mail.fl_str_mv *
_version_ 1873869535420874752
spelling Parameters for the germination of elite fungal biocontrol strainsParámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladoresUribe Gutiérrez, LizSantos-Diaz , Adriana conidioscalidadbioinsumosviabilidadconidiaqualitybiopesticidesviabilityThe marketing of microbial pesticides has increased exponentially in recent years. Almost 70% of the products marketed use as active ingredient conidia of biocontrol fungi, and One of the most frequently used criteria to evaluate the quality of these products is conidial germination test, Specifically, this methodology is used to select and assess the viability and vigor of elite strains with biological potential. Some of the factors that influence conidial germination over time, include the selection of culture medium, temperature, incubation time, and the addition of germination synchronizing agents. The optimization for each elite strain allows the characterization of a microorganism behavior over time, ensuring its biological activity and effective management as a biological resource for commercial purposes. The main objective of this study was to identify the optimal conditions (culture media, incubation time, and synchronizing agent concentration) for the germination of three elite strains: Trichoderma koningiopsis (Th003), Lecanicillium lecanii (Vl026), and Metarhizium robertsii (Mt004), which are active ingredients in biopesticides. As a result, the optimal conditions selected are as follows: agar-water medium with 0.0003 % benomyl for Th003 with an incubation period of 18 hours, agar-water medium with 0.0003 % benomyl for Vl026 with an incubation period of 24 hours, and PDA agar supplemented with 0.0003 % benomyl for Mt004 with an incubation period of 18 hours. These conditions will allow a precise evaluation of the germination of the biological control agents ensuring over time their viability and vigor as active ingredients in biopesticides.La comercialización de plaguicidas microbianos ha aumentado de manera exponencial en los últimos años. Casi el 70 % de los productos que se comercializan utilizan como principio activo conidios de hongos biocontroladores y uno de los criterios más utilizados para evaluar la calidad de estos productos es la prueba de germinación de conidios. Específicamente, se utiliza para la selección y la evaluación de la viabilidad y vigor de cepas élite con potencial biológico. Algunos de los factores que influyen en la germinación de los conidios incluye la selección del medio del cultivo, la temperatura, el tiempo de incubación y la adición de sustancias sincronizadoras de germinación. La optimización para cada cepa élite permite estimar el comportamiento del microorganismo, lo que garantizará su actividad biológica y eficacia, al ser un recurso biológico con fines comerciales. El objetivo de este trabajo fue seleccionar las condiciones óptimas (medios de cultivo, tiempo de incubación y concentración de sustancia sincronizadora) para la germinación de tres cepas élite Trichoderma koningiopsis (Th003), Lecanicillium lecanii (Vl026) y Metarhizium robertsii (Mt004), las cuales son principios activos de bioplaguicidas en proceso de comercialización. Como resultado se seleccionó el medio de cultivo de agar agua con benomil al 0,0003 % con un tiempo de lectura de 18 h para Th003 y de 24 h para Vl026. Para la cepa Mt004, el medio de cultivo que favoreció la germinación sincrónica de los conidios fue agar PDA suplementado con benomil al 0,0003 % con un tiempo de lectura de 18 h. Estas condiciones permitirán a través del tiempo realizar una correcta evaluación de la germinación de los agentes de control biológico y garantizar su viabilidad y vigor como principio activo de bioplaguicidas.Universidad Central del Ecuador2024-02-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdftext/htmlapplication/ziptext/xmlhttps://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/563110.29166/siembra.v11i1.5631Siembra; Vol. 11 No. 1 (2024); e5631Siembra; Vol. 11 Núm. 1 (2024); e56312477-88501390-892810.29166/siembra.v11i1reponame:Revista Siembrainstname:Universidad Central del Ecuadorinstacron:UCEspaenghttps://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/7650https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/9882https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/8251https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/8252https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/5631/7922Derechos de autor 2024 Liz Uribe Gutiérrez, Adriana Santos-Diaz https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccess2025-03-18T19:45:43Zoai:revistadigital.uce.edu.ec:article/5631Portal de revistashttps://revistadigital.uce.edu.ec/Universidad públicahttps://uce.edu.ec/**Ecuador*2477-88501390-8928opendoar:*2026-08-09T20:39:15.800185Revista Siembra - Universidad Central del Ecuadortrue
score 14,717255

Registros relacionados