Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance

Arbuscular mycorrhizal fungi [AMF] establish mutualistic symbioses with plant roots, enhancing nutrient and water uptake in exchange for carbohydrates, which boosts plant growth, stress resistance, and ecosystem health. This study focused on developing a biological inoculant from native AMF of a dry...

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Autores: Naranjo-Moran, Jaime, Vargas-Huacon, Nicolle, Morales-Núñez, Allison
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:Ecuador
Institución:Universidad Central del Ecuador
Repositorio:Revista Siembra
Idioma:español
inglés
OAI Identifier:oai:revistadigital.uce.edu.ec:article/7691
Acceso en línea:https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691
Access Level:acceso abierto
Palabra clave:biofertilizante
bioprospección
colonización micorrízica
esporas
simbiosis
biofertilizer
bioprospecting
mycorrhizal colonization
spores
symbiosis
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dc.title.none.fl_str_mv Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
Evaluación de hongos micorrízicos arbusculares aislados en la rizosfera de dos plantas silvestres inoculados en Zea mays y Phaseolus vulgaris, cultivos de importancia económica
title Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
spellingShingle Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
Naranjo-Moran, Jaime
biofertilizante
bioprospección
colonización micorrízica
esporas
simbiosis
biofertilizer
bioprospecting
mycorrhizal colonization
spores
symbiosis
title_short Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
title_full Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
title_fullStr Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
title_full_unstemmed Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
title_sort Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importance
dc.creator.none.fl_str_mv Naranjo-Moran, Jaime
Vargas-Huacon, Nicolle
Morales-Núñez, Allison
author Naranjo-Moran, Jaime
author_facet Naranjo-Moran, Jaime
Vargas-Huacon, Nicolle
Morales-Núñez, Allison
author_role author
author2 Vargas-Huacon, Nicolle
Morales-Núñez, Allison
author2_role author
author
dc.subject.none.fl_str_mv biofertilizante
bioprospección
colonización micorrízica
esporas
simbiosis
biofertilizer
bioprospecting
mycorrhizal colonization
spores
symbiosis
topic biofertilizante
bioprospección
colonización micorrízica
esporas
simbiosis
biofertilizer
bioprospecting
mycorrhizal colonization
spores
symbiosis
description Arbuscular mycorrhizal fungi [AMF] establish mutualistic symbioses with plant roots, enhancing nutrient and water uptake in exchange for carbohydrates, which boosts plant growth, stress resistance, and ecosystem health. This study focused on developing a biological inoculant from native AMF of a dry forest, utilizing Rhynchosia minima and Cenchrus echinatus as trap plants for their collection. The methodology involved the identification of arbuscular mycorrhizae from the rhizospheric soil of each plant species, where root and soil samples were collected, followed by the preparation of the inoculum with antibiotics (gentamicin and streptomycin). Inoculation was performed by direct application of the inoculum to the roots of maize (Zea mays) and bean (Phaseolus vulgaris) plants in a randomized block design with three treatments (T1 = control without mycorrhizal inoculum; T2 = mycorrhizae + antibiotic; T3 = mycorrhizal inoculum only) and six replicates; the sterilized substrate consisted of a 1:1 ratio of peat and sand. Plant growth and mycorrhizal colonization were evaluated and compared using Student's t-test. The results revealed a mycorrhizal colonization exceeding 50% and a spore abundance (101–132 spores g⁻¹ of soil) in the trap plants, with a predominance of the genera Acaulospora, Glomus, Gigaspora, and Scutellospora. Inoculation with these native AMF increased colonization in maize (32%) and beans (37%) compared to the non-inoculated controls of each species, demonstrating the effectiveness of the developed inoculant. In conclusion, the use of native arbuscular mycorrhizal fungi represents an effective strategy to improve the growth of important crops such as maize and beans, contributing to more sustainable agricultural practices. 
publishDate 2026
dc.date.none.fl_str_mv 2026-06-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691
10.29166/siembra.v13i1.7691
url https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691
identifier_str_mv 10.29166/siembra.v13i1.7691
dc.language.none.fl_str_mv spa
eng
language spa
eng
dc.relation.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12359
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12603
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12461
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12462
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12463
dc.rights.none.fl_str_mv Derechos de autor 2026 Jaime Naranjo-Moran, Nicolle Vargas-Huacon, Allison Morales-Núñez
https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2026 Jaime Naranjo-Moran, Nicolle Vargas-Huacon, Allison Morales-Núñez
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/html
application/zip
text/xml
dc.publisher.none.fl_str_mv Universidad Central del Ecuador
publisher.none.fl_str_mv Universidad Central del Ecuador
dc.source.none.fl_str_mv Siembra; Vol. 13 No. 1 (2026); e7691
Siembra; Vol. 13 Núm. 1 (2026); e7691
2477-8850
1390-8928
reponame:Revista Siembra
instname:Universidad Central del Ecuador
instacron:UCE
instname_str Universidad Central del Ecuador
instacron_str UCE
institution UCE
reponame_str Revista Siembra
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repository.name.fl_str_mv Revista Siembra - Universidad Central del Ecuador
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spelling Evaluation of arbuscular mycorrhizal fungi isolated from the rhizosphere of two wild plants inoculated in Zea mays and Phaseolus vulgaris, crops of economic importanceEvaluación de hongos micorrízicos arbusculares aislados en la rizosfera de dos plantas silvestres inoculados en Zea mays y Phaseolus vulgaris, cultivos de importancia económicaNaranjo-Moran, JaimeVargas-Huacon, NicolleMorales-Núñez, Allisonbiofertilizantebioprospeccióncolonización micorrízicaesporassimbiosisbiofertilizerbioprospectingmycorrhizal colonizationsporessymbiosisArbuscular mycorrhizal fungi [AMF] establish mutualistic symbioses with plant roots, enhancing nutrient and water uptake in exchange for carbohydrates, which boosts plant growth, stress resistance, and ecosystem health. This study focused on developing a biological inoculant from native AMF of a dry forest, utilizing Rhynchosia minima and Cenchrus echinatus as trap plants for their collection. The methodology involved the identification of arbuscular mycorrhizae from the rhizospheric soil of each plant species, where root and soil samples were collected, followed by the preparation of the inoculum with antibiotics (gentamicin and streptomycin). Inoculation was performed by direct application of the inoculum to the roots of maize (Zea mays) and bean (Phaseolus vulgaris) plants in a randomized block design with three treatments (T1 = control without mycorrhizal inoculum; T2 = mycorrhizae + antibiotic; T3 = mycorrhizal inoculum only) and six replicates; the sterilized substrate consisted of a 1:1 ratio of peat and sand. Plant growth and mycorrhizal colonization were evaluated and compared using Student's t-test. The results revealed a mycorrhizal colonization exceeding 50% and a spore abundance (101–132 spores g⁻¹ of soil) in the trap plants, with a predominance of the genera Acaulospora, Glomus, Gigaspora, and Scutellospora. Inoculation with these native AMF increased colonization in maize (32%) and beans (37%) compared to the non-inoculated controls of each species, demonstrating the effectiveness of the developed inoculant. In conclusion, the use of native arbuscular mycorrhizal fungi represents an effective strategy to improve the growth of important crops such as maize and beans, contributing to more sustainable agricultural practices. Los hongos micorrízicos arbusculares [HMA] establecen simbiosis mutualistas con las raíces de las plantas, mejorando la absorción de nutrientes y agua a cambio de carbohidratos, lo que potencia el crecimiento vegetal, la resistencia al estrés y la salud del ecosistema. Este estudio se enfocó en desarrollar un inoculante biológico a partir de HMA nativos de un bosque seco, utilizando Rhynchosia minima y Cenchrus echinatus como plantas trampa para su obtención. La metodología implicó la identificación de las micorrizas arbusculares de suelo rizosférico de cada especie vegetal, donde se recolectó muestras de raíz y suelo, para luego realizar preparación del inóculo con antibióticos (gentamicina y estreptomicina). La inoculación se realizó mediante la aplicación directa del inóculo a las raíces de plantas de maíz (Zea mays) y fréjol (Phaseolus vulgaris) en un diseño de bloques aleatorizados con tres tratamientos (T1 = control sin inóculo de micorrizas; T2 = micorrizas + antibiótico; T3 = solo con inóculo micorrizas) y seis réplicas, el sustrato fue esterilizado estaba constituido por una proporción 1:1 de turba y arena. El crecimiento vegetal y la colonización micorrízica se evaluaron y compararon mediante la prueba t de Student. Los resultados revelaron una colonización micorrízica superior al 50% y una abundancia de esporas (101-132 esporas g-1 de suelo) en las plantas trampa, con predominio de los géneros Acaulospora, Glomus, Gigaspora y Scutellospora. La inoculación con estos HMA nativos incrementó la colonización en maíz (32%) y fréjol (37%) en comparación con los controles de cada especie no inoculados, demostrando la efectividad del inoculante desarrollado. En conclusión, el uso de hongos micorrízicos arbusculares nativos representa una estrategia eficaz para mejorar el crecimiento de cultivos importantes como el maíz y el fréjol, contribuyendo a prácticas agrícolas más sostenibles. Universidad Central del Ecuador2026-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdftext/htmlapplication/ziptext/xmlhttps://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/769110.29166/siembra.v13i1.7691Siembra; Vol. 13 No. 1 (2026); e7691Siembra; Vol. 13 Núm. 1 (2026); e76912477-88501390-8928reponame:Revista Siembrainstname:Universidad Central del Ecuadorinstacron:UCEspaenghttps://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12359https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12603https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12461https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12462https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/7691/12463Derechos de autor 2026 Jaime Naranjo-Moran, Nicolle Vargas-Huacon, Allison Morales-Núñezhttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccess2026-07-16T13:54:05Zoai:revistadigital.uce.edu.ec:article/7691Portal de revistashttps://revistadigital.uce.edu.ec/Universidad públicahttps://uce.edu.ec/**Ecuador*2477-88501390-8928opendoar:*2026-08-09T20:39:16.849875Revista Siembra - Universidad Central del Ecuadortrue
score 15,228081

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