Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani

Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the ven...

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Detalles Bibliográficos
Autores: Arni, Raghuvir K., Fontes, Marcos Roberto de Mattos, Barberato, C., Gutiérrez, José María, Díaz Oreiro, Cecilia, Ward, Richard John
Tipo de recurso: artículo
Fecha de publicación:1999
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/29449
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S0003986199912109
https://hdl.handle.net/10669/29449
Access Level:acceso embargado
Palabra clave:Lys49-Phospholipase A2
Cerrophidion (Bothrops) godmani
Myotoxins
Snake venom
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spelling Arni, Raghuvir K.ee6da8e5-11e8-4716-b8e1-faab01daadb6600Fontes, Marcos Roberto de Mattos70aa7e08-eb67-47cd-a5a0-8a78a3d034f6600Barberato, C.788ee723-4fe9-426e-8270-f807b0e5391c-1Gutiérrez, José María6a9bac5f-130f-4977-8ddf-ab97fcafacc4600Díaz Oreiro, Ceciliaf49daa4d-080c-4cf2-9856-a48ede706fba600Ward, Richard John54bbd462-070f-44db-adb8-12d1e8bf3b26-12017-01-23T16:08:35Z2017-01-23T16:08:35Z1999-06-15http://www.sciencedirect.com/science/article/pii/S00039861999121090003-9861https://hdl.handle.net/10669/2944910.1006/abbi.1999.1210Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.Fundação de Amparo à Pesquisa do Estado de São Paulo//FAPESP/BrasilFundação para O Desenvolvimento da Unesp//FUNDUNESP/BrasilConselho Nacional de Desenvolvimento Científico e Tecnológico//CNPq/BrasilUniversidad de Costa Rica//UCR/Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)en_USacceso embargadohttp://purl.org/coar/access_right/c_f1cfinfo:eu-repo/semantics/embargoedAccessArchives of Biochemistry and Biophysics; Volumen 366, Número 2, 1999reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRLys49-Phospholipase A2Cerrophidion (Bothrops) godmaniMyotoxinsSnake venomCrystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmaniartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleTHUMBNAIL148_1999_ABB_Arni_PLA2_C.godmani_3Dstructure.pdf.jpg148_1999_ABB_Arni_PLA2_C.godmani_3Dstructure.pdf.jpgGenerated Thumbnailimage/jpeg5360https://www.kerwa.ucr.ac.cr/bitstreams/41de23de-6346-4d5f-9611-a2cbbda8eb19/downloadb4b76e9f3d07a892325658e20e1dc71aMD54ORIGINAL148_1999_ABB_Arni_PLA2_C.godmani_3Dstructure.pdf148_1999_ABB_Arni_PLA2_C.godmani_3Dstructure.pdfapplication/pdf4256051https://www.kerwa.ucr.ac.cr/bitstreams/8609ac1b-b144-4517-ba7e-9b9d4d904d73/downloadcc54003cffb77bfa461aa7c9700ee980MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82437https://www.kerwa.ucr.ac.cr/bitstreams/fdc24571-6b2a-4240-9772-66951aa09c2f/downloadf390d89ef134b2cf93d74a0f1594d4aeMD52TEXT148_1999_ABB_Arni_PLA2_C.godmani_3Dstructure.pdf.txt148_1999_ABB_Arni_PLA2_C.godmani_3Dstructure.pdf.txtExtracted texttext/plain6https://www.kerwa.ucr.ac.cr/bitstreams/18c19e93-7840-41f9-a414-e9f7ec68124b/download6d93d3216dc4a7f5df47d4876fbec4d3MD5310669/294492021-04-12 09:26:05.462embargo2069-06-01oai:kerwa.ucr.ac.cr:10669/29449https://www.kerwa.ucr.ac.crInstitucionalhttp://www.kerwa.ucr.ac.crUniversidadhttp://www.ucr.ac.crhttps://kerwa.ucr.ac.cr/oai/requestmeilyn.garro@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:18712025-03-29T18:04:19.812Kérwá - Universidad de Costa Ricafalse
dc.title.es_ES.fl_str_mv Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
title Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
spellingShingle Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
Arni, Raghuvir K.
Lys49-Phospholipase A2
Cerrophidion (Bothrops) godmani
Myotoxins
Snake venom
title_short Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
title_full Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
title_fullStr Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
title_full_unstemmed Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
title_sort Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
dc.creator.none.fl_str_mv Arni, Raghuvir K.
Fontes, Marcos Roberto de Mattos
Barberato, C.
Gutiérrez, José María
Díaz Oreiro, Cecilia
Ward, Richard John
author Arni, Raghuvir K.
author_facet Arni, Raghuvir K.
Fontes, Marcos Roberto de Mattos
Barberato, C.
Gutiérrez, José María
Díaz Oreiro, Cecilia
Ward, Richard John
author_role author
author2 Fontes, Marcos Roberto de Mattos
Barberato, C.
Gutiérrez, José María
Díaz Oreiro, Cecilia
Ward, Richard John
author2_role author
author
author
author
author
dc.subject.es_ES.fl_str_mv Lys49-Phospholipase A2
Cerrophidion (Bothrops) godmani
Myotoxins
Snake venom
topic Lys49-Phospholipase A2
Cerrophidion (Bothrops) godmani
Myotoxins
Snake venom
description Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.
publishDate 1999
dc.date.issued.none.fl_str_mv 1999-06-15
dc.date.accessioned.none.fl_str_mv 2017-01-23T16:08:35Z
dc.date.available.none.fl_str_mv 2017-01-23T16:08:35Z
dc.type.none.fl_str_mv artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv http://www.sciencedirect.com/science/article/pii/S0003986199912109
dc.identifier.issn.none.fl_str_mv 0003-9861
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/29449
dc.identifier.doi.none.fl_str_mv 10.1006/abbi.1999.1210
url http://www.sciencedirect.com/science/article/pii/S0003986199912109
https://hdl.handle.net/10669/29449
identifier_str_mv 0003-9861
10.1006/abbi.1999.1210
dc.language.iso.es_ES.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv acceso embargado
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eu_rights_str_mv embargoedAccess
dc.source.es_ES.fl_str_mv Archives of Biochemistry and Biophysics; Volumen 366, Número 2, 1999
dc.source.none.fl_str_mv reponame:Kérwá
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Kérwá
collection Kérwá
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